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Staining
Pioneers in the industry, we offer pro techex-acid fast staining teaching kit, pro techex-gram staining teaching kit, pro techex-h&e staining teaching kit, pro techex-elastic tissue fibers verhoeff s van gieson s (evg) teaching kitit, pro techex-van gieson staining teaching kit and pro techex-oil red o staining teaching kit from India.Pro TechEx-Acid Fast Staining Teaching Kit
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Approx. Rs 4,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | Progene |
| Usage/Application | Laboratory |
| Catalog Number | OP-4101-25xp |
| Material | Reagents |
| Shelf Life | 12 Months |
| Country of Origin | Made in India |
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis. The lipoid capsule of the acid – fast organism takes up carbolfuchsin and resists decolorization with a dilute acid rinse. The lipoid capsule of the mycobacteria is of such high molecular weight that is waxy at room temperatureand successful penetration by the aqueous based staining solutions (such as gram’s) is prevented .
| Cat.No | Product | Experiments |
| OP-4101-25xp | Pro TechEx-Acid Fast Staining Teaching Kit | 25 Exps |
Components:
Sample culture (4°C)Primary stain (RT)Decolorizer (RT)Counter stain (RT)Glass slides (RT)Absorbent paper (RT)Protocol
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Pro TechEx-Gram Staining Teaching Kit
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Approx. Rs 4,200 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 25 Kit |
| ICMR Approved | No |
| Usage/Application | Research and Institute |
| Packagint Type | Pack |
| Packaging Size | 25xp |
| Form | Liquid |
| Catalog Number | OP-4105-25xp |
| Material | Plastic |
| Brand | PROGENE |
Gram staining, also called Gram's Method, is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative). The name comes from its inventor, Hans Christian Gram.Gram staining consists of four components
- Primary stain (Crystal violet, methyl violet or Gentian violet)
- Mordant (Gram's Iodine)
- Decolourizer (ethyl alcohol, acetone or 11 ethanol-acetone mixture)
- Counterstain (Dilute carbol fuchsin, safranin or neutral red)
| CAT.NO | PRODUCT | EXPS |
| OP-4105-25xp | Pro TechEx-Gram Staining Teaching Kit | 25Exps |
Components
- Primary Stain (RT)
- Mordant (RT)
- Decolourizer (RT)
- Secondary Stain (RT)
- Glass slides (RT)
- Filter paper (RT)
- Protocol
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Pro TechEx-H&E Staining Teaching Kit
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Approx. Rs 5,100 / KitGet Latest Price
Product BrochureProduct Details:| Packaging Size | Pack of 10 |
| ICMR Approved | No |
| Accuracy | 0.9 |
| Disposable/ Reusable | Disposable |
| Shelf Life | 12 Months |
| Usage/Application | Laboratory |
| Country of Origin | Made in India |
| Packagint Type | Box |
| Packaging Type | Pack |
The principle behind H & E stain is the chemical attraction between tissue and dye. Hematoxylin, a basic dye imparts blue-purple contrast on basophilic structures, primarily those containing nucleic acid moeties such as chromtatin, ribosomes and cytoplasmic regions rich in RNA. An acidic eosin counterstains the basic elements such as RBCs, cytoplasm, muscle and collagen in varying intensities of pink, orange and red.
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Pro TechEx-Elastic Tissue Fibers Verhoeff s Van Gieson s (EVG) Teaching Kitit
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Approx. Rs 7,100 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| ICMR Approved | No |
| Packagint Type | Box |
| Catalog Number | OP-4119-25xp |
| Shelf Life | 12 Months |
| Packaging Type | 25xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
The tissue is stained with a regressive hematoxylin, consisting of ferric chloride and iodine. The differentiating is accomplished by using excess mordant (ferric chloride) to break the tissue-mordant dye complex. The dye will be attracted to the larger amount of mordant in the differentiating solution and will be removed from the tissue. The elastic tissue has the strongest affinity of the iron- hematoxylin complex and will retain the dye longer than the other tissue elements.
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Pro TechEx-Van Gieson Staining Teaching Kit
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Approx. Rs 2,900 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 20 Kit |
| ICMR Approved | No |
| Number of Reactions(Preps)/Kit | 20 |
| Packaging Size | 20 |
| Catalog Number | OP-4114-20xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
Van Gieson is used to differentiate between collagen and smooth muscle in tumours and to demonstrate the increase of collagen in diseases. When using combined solutions of picric acid and acid fuchsin, the small molecules of picric acid penetrate all of the tissues rapidly, but are only firmly retained in the close textured, red blood cells and muscle. The larger molecules of ponceau S displaces picric acid molecules from collagen fibres, which have larger pores, and allow the larger molecules to enter.
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Pro TechEx-Oil red O Staining Teaching Kit
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Approx. Rs 5,200 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Staining |
| Material | Teaching Kit |
| Brand | PROGENE OP-4112-10xp |
| Type | Oil Red O Staining Teaching Kit |
| Portable | Yes |
| Country of Origin | Made in India |
The histological mechanism of the staining of lipids is invariably a function of the physical properties of the dye being more soluble in the lipid to be demonstrated than in the vehicular solvent. The poly azo group of dyes include the oil red series, the Sudan red series, and the Sudan blacks. All these dyes are interchangeable, and may be substituted in the above method.
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Pro TechEx-Simple Staining Method for Bacteria in Milk Teaching Kit
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Approx. Rs 3,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Catalog Number | OP-4110-25xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
Milk is fermented by inoculating appropriate bacteria. The bacteria that developed can be stained by simple staining method.Basic stains with a positively charged chromogen are preferred because bacterial nucleic acids and certain cell wall components carry a negative charge that strongly attracts and binds to the cationic chromogen. The purpose of simple staining is to elucidate the morphology and arrangements of bacterial cells in milk. The commonly used basic stains are Methylene Blue, Crystal Violet, and Carbol Fuchsin.
| Cat. No | Product | Experiments |
| OP-4110-25xp | Pro TechEx-Simple Staining Method For Bacteria In Milk Teaching Kit | 25 Exps |
| Components |
| Methylene Blue (RT) |
| Crystal violet (RT) |
| Carbol fuchsin (RT) |
| Protocol |
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Pro TechEx-Endospore Staining Teaching Kit
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Approx. Rs 4,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Packaging Size | 25 |
| Number of Reactions(Preps)/Kit | 25 |
| Catalog Number | OP-4103-25xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample, which can be useful for classifying bacteria. Within bacteria, endospores are quite protective structures used to survive extreme conditions, but this protective nature makes them difficult to stain using normal techniques. Special techniques for endospore staining include the Schaeffer–Fulton stain and the Moeller stain. A good stain to use for spore staining is malachite green. It takes a long time for the spores to stain due to their density, so time acts as the mordant when doing this differential stain; the slide with the bacterium should be soaked in malachite green for at least 30 minutes. Water acts as the decolorizer. A counterstain to differentiate the vegetative cells is 0.5% safranin. Types of endospores that could be identified are free endospores, central endospores, central and swollen endospores, and subterminal endospores.
| CAT.NO | PRODUCT | EXPS |
| OP-4103-25xp | Pro TechEx-Endospore Staining Teaching Kit | 25Exps |
Components
- Primary stain (RT)
- Counter stain (RT)
- Glass slide (RT)
- Bacillus culture (4°C)
- Filter paper (RT)
- Protocol
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Pro TechEx-Collagen Masson Trichrome Teaching Kit
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Approx. Rs 5,900 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Number of Reactions(Preps)/Kit | 10 |
| Packaging Size | 10 |
| Catalog Number | OP-4113-10xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Used to differentiate between collagen and smooth muscle in tumors, and the increase of collagen in diseases such as cirrhosis. Routine stain for liver and kidney biopsies.
| Cat. No | Product | Experiments |
| OP-4113-10xp | Pro Tech Ex- Collagen Masson Trichrome kit | 10 Exps |
| Components | STORAGE |
| 100% alcohol | RT |
| 10% formalin | RT |
| Weigert's iron hematoxylin | RT |
| Biebrich scarlet-acid fuchsin | RT |
| phosphomolybdic-phosphotungstic acid | RT |
| aniline blue | RT |
| 1% acetic acid solution | RT |
| DPX mounting medium | RT |
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Pro TechEx-Simple Staining Teaching Kit
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Approx. Rs 3,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 25 Kit |
| ICMR Approved | No |
| Packagint Type | Pack |
| Packaging Size | 25 |
| Catalog Number | OP-4111-25xp |
| Shelf Life | 12 Months |
| Brand | PROGENE |
| Country of Origin | Made in India |
The purpose of simple staining is to elucidate the morphology and arrangements of bacterial cells in milk. The commonly used basic stains are Methelene Blue, Crystal Violet, and Carbol Fuchsin.
| CAT.NO | PRODUCT | EXPS |
| OP-4111-25xp | Pro TechEx-Simple Staining Teaching Kit | 25Exps |
Components
- Methylene blue (RT)
- Glass slides (RT)
- Bacterial Culture (4 DegreeC)
- Absorbent papers (RT)
- Protocol
MOQ 25 Exps
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Pro TechEx-Flagella Staining Teaching Kit
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Approx. Rs 4,200 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | Progene |
| Usage/Application | Laboratory |
| Packaging Size | 25xp |
| Catalog Number | OP-4104-25xp |
Bacterial flagella are fine, thread like organelles for locomotion. They are slender (about 10 to 30nm in diameter) and can only be seen directly using the electron microscope. In order to observe them with the light microscope, the thickness of the flagella are increased by coating them with mordants like tannic acid and potassium alum, and staining them with basic fuchsin (Gray method), pararosaniline (Leifson method), silver nitrate (West method) or crystal violet (Difco's method). Although flagella staining procedures are difficult to carry out, they often provide information about the presence and location of flagella, which is of great value in bacterial identification.
| Cat.No | Product | Experiments |
| OP-4104-25xp | Pro TechEx-Flagella Staining Teaching Kit | 25 Exps |
Components:
Bacterial culture (4°C)Primary stain (RT)Counter stain (RT)Glass Slides (RT)Pasteur pipette (RT)
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Pro TechEx-Albert Staining Teaching Kit
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Approx. Rs 4,300 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Packagint Type | Box |
| Catalog Number | OP-4116-25xp |
| Shelf Life | 12 Months |
| Packaging Type | PROGENE |
| Brand | PROGENE |
| Country of Origin | Made in India |
Albert staining technique aims at detecting the presence of metachromatic granulated bodies of Corynebacterium diphtheriae. Albert stain is made up of two staining solutions; designated as Albert Solution 1 and Albert Solution 2.
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Pro TechEx-Lactophenol Cotton Blue Staining Teaching Kit
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Approx. Rs 3,300 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Size | 25xp |
| Model Name/Number | OP-4106-25xp |
| Brand | Progene |
| Country of Origin | Made in India |
Fungi are very difficult to visualize without the aid of special Staining procedure. Lactic acid act as a preservative for fungi. The phenol portion kills the fungi. The cotton blue stains the fungal elements that results Fungal elements are stained a deep blue; background is pale blue. Do not overstain.
| Cat.No | Product | Experiments |
| OP-4106-25xp | Pro TechEx-Lactophenol Cotton Blue Staining Teaching Kit | 25 Exps |
Components:
Fungal culture (4°C)lactophenol stain (RT)Glass slide (RT)Applicator sticks(RT)coverslip(RT)Protocol
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Pro TechEx-Metachromatic Granules Staining Teaching Kit
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Approx. Rs 3,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Medical |
| Model Name/Number | OP-4107-25xp |
| Brand | Progene |
| Country of Origin | Made in India |
Albert's staining technique is a type of special staining technique since it is used to demonstrate a special structure in bacteria. It is chiefly used to demonstrate metachromatic granules found in Corynebacterium diphtheriae. The storage granules in this bacterium is called metachromatic granules because it exhibits the property of metachromasia, wherein the granules appear in a colour other than the colour used for staining. When stained with polychrome methylene blue, the granules appear violet while the rest of the bacillus appears blue. Green coloured, rod shaped bacteria that are arranged at angles to each other resembling English letter 'L', 'V' or Chinese letter pattern along with bluish black metachromatic granules at the poles seen.
| Cat.No | Product | Experiments |
| OP-4107-25xp | Pro TechEx-Metachromatic Granules Staining Teaching Kit | 25 Exps |
Components:
Bacterial culture (4°C)Glass slide (RT)Albert stain (RT)Absorbent paper (RT)Protocol
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Pro TechEx-Papanicolaou (PAP) Staining Teaching Kit
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Approx. Rs 5,750 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Kit |
| ICMR Approved | No |
| Packagint Type | Pack |
| Packaging Size | 25xp |
| Grade Standard | Progene |
| Catalog Number | OP-4117-25xp |
| Shelf Life | 12 Months |
| Country of Origin | Made in India |
Papanicolaou stain (also Papanicolaou's stain and Pap stain) is a multichromatic staining histological technique developed by George Papanikolaou, the father of cytopathology. Pap staining is used to differentiate cells in smear preparations of various bodily secretions; the specimens can be gynecological smears (Pap smears), sputum, brushings, washings, urine, cerebrospinal fluid, abdominal fluid, pleural fluid, synovial fluid, seminal fluid, fine needle aspiration material, tumor touch samples, or other materials containing cells. Pap staining is a very reliable technique. As such, it is used for cervical cancer screening in gynecology. The entire procedure is known as Pap smear.
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Pro TechEx- Capsular Staining Teaching Kit
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Approx. Rs 4,100 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Model Name/Number | OP-4102-25xp |
| Brand | Progene |
| Country of Origin | Made in India |
Many bacteria have a slimy layer surrounding them,which is usually referred to as a capsule. The capsule's composition, as well as its thickness, varies with individual bacterial species. Polysaccharides, polypeptides, and glycoproteins have all been found in capsules. Often, a pathogenic bacterium with a thick capsule will be more virulent than a strain with little or no capsule since the capsule protects the bacterium against the phagocytic activity of the host's phagocytic cells. The capsule stain is of some importance in clinical microbiology (e.g., in the diagnosis of bacterial pneumonia and the fungus Cryptococcus neoformans) Anthony's procedure employs two reagents. The primary stain is crystal violet, which gives the bacterial cell and its capsular material a dark purple color.Unlike the cell, the capsule is nonionic and the primary stain cannot adhere. Copper sulfate is the decolorizing agent. It removes excess primary stain as well as color from the capsule. At the same time, the copper sulfate acts as a counterstain by being absorbed into the capsule and turning it a light blue. In this procedure, smears should not be heat-fixed since shrinkage is likely to occur and create a clear zone around the bacterium, which can be mistaken for a capsule.
| Cat.No | Product | Experiments |
| OP-4102-25xp | Pro TechEx-Capsular Staining Teaching Kit | 25 Exps |
Components:
Culture (4°C)Primary Stain (RT)Copper sulphate solution (20%).(RT)Glass Slide (RT)Filter paper (RT)Protocol
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Pro TechEx - Oxidase Test Teaching Kit
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Approx. Rs 3,300 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Kit |
| ICMR Approved | No |
| Temprature | RT |
| Usage/Application | Laboratory |
| Packagint Type | Box |
| Sterile/ Non Sterile | Sterile |
| Packaging Size | 10 |
| Shelf Life | 12 Months |
| Country of Origin | Made in India |
The oxidase test is based on bacterial production of an intracellular oxidase enzyme and some organisms may produce more than one type of oxidase enzyme. These enzymes participate in the cellular
respiration process and catalyze removal of hydrogen from a substrate using oxygen as a hydrogen acceptor. The active substrate in oxidase reagent, N,N,N,Ntetramethyl-p-phenylenediamine dihydrochloride, acts as an artificial electron acceptor for the enzyme oxidase and is oxidized to form the colored compound Wurster’s blue. Wurster’s blue is a purple compound
that is readily visible and signifies a positive reaction. This formulation was devised by Kovacs and is often referred to as Kovacs Oxidase Reagent.
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Pro TechEx-Monochrome Staining Teaching Kit
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Approx. Rs 3,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Number of Reactions(Preps)/Kit | 25 |
| Packaging Size | 25 |
| Catalog Number | OP-4108-25xp |
| Brand | PROGENE |
The use of single stain to colour the bacteria is commonly called as monochrome staining. (Mono-single, Chrome-color)The method of staining utilizes the knowledge that bacteria possess slight negative charge. The negative charged group of bacterial cell surface produces attraction between basic stains. The stains used in this method include methylene blue, crystal violet,etc .
| CAT.NO | PRODUCT | EXPS |
| OP-4108-25xp | Pro TechEx-Monochrome Staining Teaching Kit | 25Exps |
Components
- Bacterial strain (4 DegreeC)
- Methylene blue stain (RT)
- Glass slide (RT)
- Absorbent paper (RT)
- Protocol
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Pro TechEx-Negative Staining Teaching Kit
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Approx. Rs 3,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Medical |
| Size | 25xp |
| Model Name/Number | OP-4109-25xp |
| Brand | Progene |
| Country of Origin | Made in India |
Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrast with 'positive staining', in which the actual specimen is stained. Negative stains are even simpler than simple stains because you do not have to make a smear. A drop of cells is spread on a slide and viewed without fixation. The stain is a suspension of carbon, found in India ink or nigrosin. The carbon particles are negatively-charged, as is the cell membrane. The background looks black or sepia colored and the cells remain clear, since they repel the dye. Some positively charged inclusion bodies such as sulfur may stain. This stain gives accurate information on cell morphology and capsule presence because the cells are not fixed.
| Cat.No | Product | Experiments |
| OP-4109-25xp | Pro TechEx-Negative Staining Teaching Kit | 25 Exps |
| Components |
| Bacterial strain (4°C) |
| Nigrosin (RT) |
| Negative Stain(RT) |
| Glass slides(RT) |
| Disposable Dropper(RT) |
| Protocol |
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