Molecular Biology
Providing you the best range of pro techex-genomic dna extraction from animal tissue teaching kit, pro techex-genomic dna extraction from whole blood teaching kit, pro techex-ligation teaching kit, pro techex-genomic dna extraction from animal tissue (high salt method) teaching kit, pro techex-gene cloning(blue-white screening)teaching kit and pro techex-gel extraction teaching kit with effective & timely delivery.Pro TechEx-Genomic DNA Extraction from Animal Tissue Teaching Kit
Approx. Rs 3,500 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Temprature | RT, 4C & -20C |
| Packagint Type | Box |
| Number of Reactions(Preps)/Kit | 10 |
| Packaging Size | 10 |
| Physical State | Liquid & powder form |
| Catalog Number | OP-3119-10xp |
| Product Type | KIT |
| Shelf Life | 12 Months |
| Brand | PROGENE |
| Country of Origin | Made in India |
Animal tissue is a common source of material when performing genetic analysis. The protocol below is a simple method of extracting DNA from the animal sample.
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Pro TechEx-Genomic DNA Extraction from Whole Blood Teaching Kit
Approx. Rs 3,500 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Temprature | RT, 4C & -20 |
| Packagint Type | Box |
| Brand | Progene |
| Number of Reactions(Preps)/Kit | 10 |
| Usage/Application | Laboratory |
| Packaging Size | 10 |
| Form | Liquid |
| Catalog Number | OP-3125-10xp |
| Material | Reagents |
| Result Time | 4hrs |
| Shelf Life | 12 Months |
| Country of Origin | Made in India |
DNA in the sample is released from blood cells using solution-I (Detergent). The cell wall of the blood cell is weakened by EDTA in the Solution-II. Solution-III denatures the cellular proteins. Extraction with phenol denatures the protein, Chloroform removes SDS- Protein / polysaccharide complexes as white interface between the aqueous and organic phase after centrifugation and isoamyl alcohol prevents foaming. The high molecular weight genomic DNA in aqueous phase is precipitated with 100 % ethanol or isopropanol and further the salts are removed by washing with 70% alcohol.
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Pro TechEx-Ligation Teaching Kit
Approx. Rs 3,200 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Catalog Number | OP-3133-5xp |
| Brand | PROGENE |
DNA ligation is the process of joining together two DNA molecules ends (either from the same or different molecules). Specifically, it involves creating a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another. This reaction is usually catalyzed by a DNA ligase enzyme. This enzyme will ligate DNA fragments having blunt or overhanging, complementary, 'sticky' ends. Typically, it is easier to ligate molecules with complementary sticky ends than blunt ends. T4 DNA ligase is the most commonly used DNA ligase for molecular biology techniques and can ligate 'sticky' or blunt ends. After ligation, the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation. Most restriction enzymes digest DNA asymmetrically across their recognition sequence, which results in a single stranded overhang on the digested end of the DNA fragment. The overhangs, called "sticky ends", are what allow the vector and insert to bind to each other. When the sticky ends are compatible, meaning that the overhanging base pairs on the vector and insert are complementary, the two pieces of DNA connect and ultimately are fused by the ligation reaction.
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Pro TechEx-Genomic DNA Extraction from Animal Tissue (High Salt Method) Teaching Kit
Approx. Rs 4,030 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | PROGENE |
| Number of Reactions(Preps)/Kit | 10 |
| Packaging Size | 10 |
| Shelf Life | 12 Months |
| Catalog Number | OP-3120-10xp |
| Country of Origin | Made in India |
A very simple, fast, universally reproducible method to extract high quality megabase genomic DNA from different organisms is described. This same method is applicable to extract high quality complex genomic DNA from different tissues (wheat, barley, potato, beans, pear and almond leaves as well as fungi, insects and shrimps' fresh tissue) without any modification. The method does not require expensive and environmentally hazardous reagents and equipment. It can be performed even in low technology laboratories. The amount of tissue required by this method is ∼50–100 mg. The quantity and the quality of the DNA extracted by this method is high enough to perform hundreds of PCR-based reactions and also to be used in other DNA manipulation techniques such as restriction digestion, Southern blot and cloning.
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Pro TechEx-Gene Cloning(Blue-White Screening)Teaching Kit
Approx. Rs 5,450 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Packaging Size | 5 |
| Catalog Number | OP-3118-5xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
The vectors used in cloning are manipulated in such a way that this α-complementation process serves as a marker for recombination. A multiple cloning site (MCS) is present within the lacZ sequence in the plasmid vector. This sequence can be nicked by restriction enzymes to insert the foreign DNA. When a plasmid vector containing foreign DNA is taken up by the host E. coli, the α-complementation does not occur, therefore, a functional β-galactosidase enzyme is not produced. If the foreign DNA is not inserted into the vector or if it is inserted at a location other than MCS, the lacZ gene in the plasmid vector complements the lacZ deletion mutation in the host E. coli producing a functional enzyme.
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Pro TechEx-Gel Extraction Teaching Kit
Approx. Rs 4,100 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 5 |
| Packaging Size | 5 |
| Catalog Number | OP-3117-5xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
A silica-gel membrane that selectively absorbs up to 10mg of DNA fragments in the presence of specialized binding buffers. Nucleotides, oligos, enzymes, mineral oil and other impurities do not bind to the membrane and are washed away. The DNA fragments can then be eluted off the column in small volume and used in almost any downstream applications.
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Pro TechEx-DNA Molecular Weight Determination Teaching Kit
Approx. Rs 3,900 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Kit |
| ICMR Approved | No |
| Packaging Size | 10 |
| Catalog Number | OP-3116-10xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
This is done using an agarose gel electrophoresis of known molecular weight nucleic acids (ladder) along with the protein or nucleic acid to be characterized. A linear relationship exists between the logarithm of the molecular weight of native nucleic acid, and it’s Rf. The Rf is calculated as the ratio of the distance migrated by the molecule to that migrated by a marker dye-front.
A simple way of determining relative molecular weight by electrophoresis is to plot a standard curve of Rf vs. log Mw for known samples (ladder), and read off the log Mw of the sample after measuring distance migrated on the same gel. After introducing the data of ladder and sample Rf, sample Mw is calculated.
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Pro TechEx-Agrobacterium Mediated Gene Transfer Teaching Kit
Approx. Rs 5,200 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Kit |
| ICMR Approved | No |
| Packagint Type | Box |
| Packaging Size | 5 |
| Catalog Number | OP-3178-5xp |
| Brand | Progene |
| Country of Origin | Made in India |
Plant Infection The most studied Agrobacterium disease is crown gall disease caused by A. tumefaciens. Gall formation of this disease is initiated at the flesh wound site of the host plant. From there the bacteria transfer its DNA into the host cell where it incorporates itself into the host genome.
| Cat. NO | Product | Experiments |
| OP-3178-5Xp | Agrobacterium Mediated Gene Transfer Teaching Kit | 5 Exps |
| OP-3178-10Xp | Agrobacterium Mediated Gene Transfer Teaching Kit | 10 Exps |
| Components | STORAGE |
| Antibiotics | 4°C |
| Agrobacterium slant | 4°C |
| YEP Medium | RT |
| MS Medium | 4°C |
| HgCl2+tween 20 | RT |
| Agar | RT |
| Protocol | RT |
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Pro TechEx-Oligonucleotide Purification Teaching Kit
Approx. Rs 8,200 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Product Type | KIT |
| Number of Reactions(Preps)/Kit | 5 |
| Catalog Number | OP-3140-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Oligonucleotide synthesis involves a large number of individual reactions, which leads inevitably to the accumulation of impurities such as truncated oligonucleotides and sequences containing chemically-modified bases. These impurities must be removed after synthesis
| CAT.NO | PRODUCT | EXPS |
| OP-3140-5xp | Pro TechEx-Oligonucleotide Purification Teaching Kit | 5 Exps |
| OP-3140-10xp | Pro TechEx-Oligonucleotide Purification Teaching Kit | 10 Exps |
Components
- 40% polyacrylamide (4 DegreeC)
- TEMED (4 DegreeC)
- APS (4 DegreeC)
- Oligonucleotide I& II (-20 DegreeC)
- Urea (RT)
- Butanol (RT)
- Phenol chloroform (4 DegreeC)
- Loading buffer (4 DegreeC)
- TE buffer (4 DegreeC)
- Ethanol I (RT)
- Ethanol II (RT)
- TLC plate (RT)
- 10X TEB (RT)
- UV wrap (RT)
- Protocol
MOQ 10 Exps
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Pro TechEx-Nested PCR Teaching Kit
Approx. Rs 7,000 / KitGet Latest Price
Product Details:| ICMR Approved | No |
| Brand | Progene |
| Number of Reactions(Preps)/Kit | OP-3138-5xp |
| Method | Elisa |
Nested PCR used to increase the specificity of DNA amplification. Two sets of primers are used in two successive reactions. In the first PCR, one pair of primers is used to generate DNA products, which may contain products amplified from non-target areas. The products from the first PCR are then used as template in a second PCR, using one ('hemi-nesting') or two different primers whose binding sites are located (nested) within the first set, thus increasing specificity. Nested PCR is often more successful in specifically amplifying long DNA products than conventional PCR, but it requires more detailed knowledge of the Sequence of the target.
| CAT.NO | PRODUCT | EXPS |
| OP-3138-5xp | Pro TechEx-Nested PCR Teaching Kit | 5 Exps |
| OP-3138-25xp | Pro TechEx-Nested PCR Teaching Kit | 25 Exps |
Components
- Agarose (RT)
- 50X TAE (RT)
- Ethidium Bromide (RT)
- Gel Loading Dye (RT)
- Template DNA (-20 DegreeC)
- Primer(F R) (-20 DegreeC)
- Taq Polymerase (-20 DegreeC)
- 10mM dNTP mix (-20 DegreeC)
- 10X Taq polymerase Buffer (-20 DegreeC)
- Distilled water (RT)
- Marker DNA (-20 DegreeC)
- PCR Tubes (RT)
- Protocol
25 Exp
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