Elisa Kit

Approx. Rs 5,600 / KitGet Latest Price

To detect soluble antigens, plates are coated with antigen and the binding of specific antibody-enzyme conjugates to antigen-coated plates is inhibited by test solutions containing soluble antigen. The direct assay may also be adapted as an indirect assay by substituting specific antibody for specific antibody-enzyme conjugate. The amount of specific antibody bound is then detected using a species-specific or isotype-specific conjugate as a tertiary reactant. The assay seems to be quick because only one antibody and fewer steps are used and cross-reactivity of secondary antibody is eliminated. Immunoreactivity of the primary antibody might be adversely affected by labeling with enzymes or tags and labeling primary antibodies for each specific Elisa system is time

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