BIO TECH KIT

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• Ethanol I (RT)
• Ethanol II (RT)
• Isopropanol (RT)
• Phenol/chloroform (4 DegreeC)
• RNA buffer (RT)
• RNA buffer SDS (RT)
• Yeast culture (4 DegreeC)
• YPD broth (RT)
• 10X MOPS (RT)
• Agarose (RT)
• Ethidium bromide (RT)
• Formaldehyde (RT)
• Gel loading dye (RT)
• TE buffer (RT)
• Protocol

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Approx. Rs 5,700 / KitGet Latest Price

                Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.

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Approx. Rs 4,900 / KitGet Latest Price

• 10X Equilibration Buffer (4 DegreeC)
• Column (4 DegreeC)
• Protein Mixture (-20 DegreeC)
• Protocol

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This protocol is used to denature and separate large RNA molecules (0.5–10 kb) on PAGE. Glyoxal (also called diformyl or ethanedial), the agent responsible for maintaining denaturation in this protocol, contains two carbonyl groups that react to form a cyclic ring structure with the imino and amino groups of guanine. It can also react with the amino groups of adenine and cytidine. When RNA is denatured in the presence of glyoxal, this covalent adduct prevents normal base pairing and maintains the RNA in a denatured state in PAGE gels. Once formed, these adducts are stable at room temperature at pH <7.0; thus, there is no need to add glyoxal to the gel or to the gel buffers to maintain the RNA in the denatured state. Because the fully denatured RNA migrates through gels according to its molecular mass, this method can be used to accurately size RNA molecules. Following electrophoresis and reversal of glyoxalation, the RNA can be detected using a northern hybridization procedure.

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