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BIO TECH KIT

Transcriptomics is the branch of Molecular Biology which deals with study of RNA. These Transcriptomics Teaching Kits are mainly consumed in dept of Biotechnology, where Transcriptomics is consider as a part of major subject.

Northern Blotting Teaching Kit

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Northern Blotting Teaching Kit
  • Northern Blotting Teaching Kit
  • Northern Blotting Teaching Kit
  • Northern Blotting Teaching Kit
  • +10 Northern Blotting Teaching Kit
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Approx. Rs 10,000 / PieceGet Latest Price

Product Brochure
Product Details:
MaterialPlastic
BrandProgene
I Deal InNew Only
Usage/ApplicationLaboratory
FormLiquid
MethodElisa

The term 'northern blot' actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot.

Cat.No Product Experiments 
OP-3303-5xpPro TechEx-Northern Blotting Teaching Kit5 Exps


Components
10X Running buffer (RT)
Agarose (RT)
Formaldehyde (RT)
RNA Sample buffer (RT)
20X SSC (RT)
Gel loading dye (RT)
RNA Sample (-20°C)
Protocol

Additional Information:

  • Item Code: OP-3303-5xp
  • Delivery Time: 5-7 Business days
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    Total RNA Extraction from Bacteria Teaching Kit

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    Total RNA Extraction from Bacteria Teaching Kit
    • Total RNA Extraction from Bacteria Teaching Kit
    • Total RNA Extraction from Bacteria Teaching Kit
    • Total RNA Extraction from Bacteria Teaching Kit
    • +9 Total RNA Extraction from Bacteria Teaching Kit
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    Approx. Rs 5,000 / PieceGet Latest Price

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    Product Details:
    Usage/ApplicationRNA Extraction Kit
    TypeTotal RNA Extraction from Bacteria Teaching Kit
    Country of OriginMade in India
    BrandPROGENE
    I Deal InNew Only

    Mid-exponential phase bacteria grown in liquid media (50-100µl pellet volume) were harvested by centrifugation at 13500 rpm for 20s in a Microfuge tube, washed in detergent solution. Repelleted and resuspended in RNase free distilled water. Re-extracted with water saturated phenol. Isolated RNA was visualized by Ethidium Bromide Stained Denaturing Formaldehyde gel electrophoresis.

    CAT.NOPRODUCTEXPS
    OP-3308-5xpPro TechEx-Total RNA Extraction from Bacteria Teaching Kit5 Exps
    OP-3308-20xpPro TechEx- Total RNA Extraction from Bacteria Teaching Kit20 Exps

    Components
    • Microfuge tubes (RT)
    • 10X MOPS (RT)
    • 70% Ethanol (RT)
    • Agarose (RT)
    • E.coli Host (4 DegreeC)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • Agarose (RT)
    • DEPC water (RT)
    • LB medium (RT)
    • Solution I (4 DegreeC)
    • Solution II (4 DegreeC)
    • Solution III (4 DegreeC)
    • Solution IV (4 DegreeC)
    • Protocol

    MOQ-20 Exps

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      Total RNA Extraction and RT-PCR Teaching Kit

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      Total RNA Extraction and RT-PCR Teaching Kit
      • Total RNA Extraction and RT-PCR Teaching Kit
      • Total RNA Extraction and RT-PCR Teaching Kit
      • Total RNA Extraction and RT-PCR Teaching Kit
      • Total RNA Extraction and RT-PCR Teaching Kit
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      Approx. Rs 13,500 / PieceGet Latest Price

      Product Brochure
      Product Details:
      Country of OriginMade in India
      BrandPROGENE
      Usage/ApplicationRNA Extraction Kit
      TypePCR Application Teaching Kit
      I Deal InNew Only
      CAT-NoOP-3307-10xp

      Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. In RT-PCR, the RNA template is first converted into a complementary DNA (cDNA) using a reverse transcriptase. The cDNA is then used as a template for exponential amplification using PCR. RT-PCR is currently the most sensitive method of RNA detection available.RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.

      CAT.NOPRODUCTEXPS
      OP-3307-10xpPro TechEx-Total RNA Extraction and RT-PCR Teaching Kit10 Exps

      Components
      • 1.5ml Microfuge tubes (RT)
      • 10mM dNTP (-20 DegreeC)
      • 10X MOPS (RT)
      • Agarose (RT)
      • Ethidium Bromide (RT)
      • Gel Loading dye (RT)
      • Primer F (-20 DegreeC)
      • Yeast Culture (4 DegreeC)
      • 0.2ml PCR tubes (RT)
      • Primer R (-20 DegreeC)
      • RNase free Water (4 DegreeC)
      • Solution I (4 DegreeC)
      • Solution II (4 DegreeC)
      • Solution III (4 DegreeC)
      • Solution IV (4 DegreeC)
      • Yeast broth (RT)

      MOQ-10 Exp

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      Total RNA Extraction From Yeast Teaching Kit

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      Total RNA Extraction From Yeast Teaching Kit
      • Total RNA Extraction From Yeast Teaching Kit
      • Total RNA Extraction From Yeast Teaching Kit
      • Total RNA Extraction From Yeast Teaching Kit
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      Approx. Rs 7,000 / PieceGet Latest Price

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      Product Details:
      Result Time1 day
      I Deal InNew Only
      BrandPROGENE
      Humidity ResistantYes
      Usage/ApplicationLaboratory
      Packaging SizeN/A


      RNA isolation is difficult procedure .Get rid of R Nases is an essential step with the help of DEPC water. The objective is to isolate a large quantity (perhaps 100 whole micrograms) of high molecular weight, undegraded total RNA. In order to further characterize the mating reaction , will be isolating total RNA from culture contains yeast cells in logarithmic growth phase grown in YPD.

      CAT.NOPRODUCTEXPS
      OP-3310-5xpPro TechEx-Total RNA Extraction From Yeast Teaching Kit5 Exps
      OP-3310-10xpPro TechEx-Total RNA Extraction From Yeast Teaching Kit10 Exps

      Components

      • Ethanol I (RT)
      • Ethanol II (RT)
      • Isopropanol (RT)
      • Phenol/chloroform (4 DegreeC)
      • RNA buffer (RT)
      • RNA buffer SDS (RT)
      • Yeast culture (4 DegreeC)
      • YPD broth (RT)
      • 10X MOPS (RT)
      • Agarose (RT)
      • Ethidium bromide (RT)
      • Formaldehyde (RT)
      • Gel loading dye (RT)
      • TE buffer (RT)
      • Protocol

      Additional Information:

    • Item Code: OP-3310
    • Delivery Time: 5-7 Business days
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      Preparation of Poly(A) mRNA From Yeast Teaching Kit

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      Preparation of Poly(A) mRNA From Yeast Teaching Kit
      • Preparation of Poly(A) mRNA From Yeast Teaching Kit
      • Preparation of Poly(A) mRNA From Yeast Teaching Kit
      • Preparation of Poly(A) mRNA From Yeast Teaching Kit
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      Approx. Rs 5,800 / PieceGet Latest Price

      Product Brochure
      Product Details:
      BrandProgene
      FormLiquid
      Usage/ApplicationLaboratory
      Packaging SizeBox
      MethodElisa
      MaterialPlastic
      I Deal InNew Only

      To isolate mRNA from total RNA sample, cellulose resin with a poly deoxythimidine oligomer attached (oligo-dT cellulose) is used to purify transcript with a poly-A tail. This purification should be done on the same day as the total RNA purification limit degradation. The total RNA and oligo-dT cellulose are incubated at 65°C and eluted by using elution buffer. Purified mRNA can be used for Reverse transcription process.


      Cat. NOProductExperiments
      OP-3306-5xpPro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit5 Exps
      OP-3306-10xpPro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit10 Exps

      Components:

      · 10X MOPS (-20°C)

      · Binding buffer (RT)

      · Elution buffer (RT)

      · Ethanol (RT)

      · Oligo dT Cellulose (-20°C)

      · Sodium acetate (RT)

      · Total RNA (-20°C)

      · 70% ethanol (RT)

      · Agarose (RT)

      · DEPC water (RT)

      · Ethidium Bromide (RT)

      · Formaldehyde (RT)

      · Gel loading Dye (RT)

      · Protocol


      Additional Information:

    • Item Code: OP-3306
    • Delivery Time: 5-7 Business days
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      Total Rna Isolation From Yeast By Trizol Method

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      Total Rna Isolation From Yeast By Trizol Method
      • Total Rna Isolation From Yeast By Trizol Method
      • Total Rna Isolation From Yeast By Trizol Method
      • Total Rna Isolation From Yeast By Trizol Method
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      Approx. Rs 5,800 / PieceGet Latest Price

      Product Brochure
      Product Details:
      Humidity ResistantYes
      BrandProgene
      FormLiquid
      Usage/ApplicationLaboratory
      Shelf Life12 Months
      Accuracy0.99
      I Deal InNew Only


      This protocol uses Trizol (also known as TRI REAGENT) for the isolation of total RNA. Trizol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, RNA and protein on homogenization or lysis of tissue sample. After adding chloroform and centrifuging, the mixture separates into 3 phases with the upper clear aqueous phase containing the RNA. The next steps in the extraction are washes and precipitation of the RNA.

      CAT.NOPRODUCTEXPS
      OP-3309-5xpPro TechEx-Total RNA isolation from by Trizol method Teaching Kit5 Exps
      OP-3309-10xpPro TechEx-Total RNA isolation from by Trizol method Teaching Kit10 Exps

      Components
      • Chloroform (RT)
      • TE buffer (RT)
      • Trizol (4 DegreeC)
      • Wash solution (RT)
      • Yeast culture (4 DegreeC)
      • 10X MOPS (RT)
      • Agarose (RT)
      • Ethidium bromide (RT)
      • Formaldehyde (RT)
      • Gel loading dye (RT)
      • Protocol

      Additional Information:

    • Item Code: OP-3309
    • Delivery Time: 5-7 Business days
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      Plant RNA Isolation Teaching Kit

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      Plant RNA Isolation Teaching Kit
      • Plant RNA Isolation Teaching Kit
      • Plant RNA Isolation Teaching Kit
      • Plant RNA Isolation Teaching Kit
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      Approx. Rs 5,000 / PieceGet Latest Price

      Product Brochure
      Product Details:
      Country of OriginMade in India
      BrandProgene
      Usage/ApplicationRNA Extraction Kit
      TypePlant RNA Isolation Teaching Kit
      Accuracy0.99
      I Deal InNew Only


      Isolation of pure, under graded total RNA from plant tissue can be difficult because of high levels of ribonucleases, pectins and polyphenols that are present in plant material. Another major problem is the presence and removal of proteins. This problem is commonly solved by lysing the plant cells in the presence of phenol/chloroform.

      CAT.NOPRODUCTEXPS
      OP-3305-5xpPro TechEx - Plant RNA Isolation Teaching Kit5 Exps
      OP-3305-10xpPro TechEx- Plant RNA Isolation Teaching Kit10 Exps

      Components
      • 10X MOPS buffer (RT)
      • Agarose (RT)
      • Chloroform Solution (4°C)
      • Ethidium Bromide (RT)
      • Extraction buffer (4°C)
      • Gel loading Dye (RT)
      • Phenol:Chloroform Solution (4°C)
      • Sodium acetate pH 5.2 (RT)
      • 70% ethanol (RT)
      • Absolute ethanol (4°C)
      • DEPC-treated water (RT)
      • Formaldehyde (RT)
      • Marker (RT)
      • Protocol

      Additional Information:

    • Item Code: OP-3305-10xp
    • Delivery Time: 5-7 Business days
    • Yes! I am interested

      Invitro Transcription Teaching Kit

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      Invitro Transcription Teaching Kit
      • Invitro Transcription Teaching Kit
      • Invitro Transcription Teaching Kit
      • Invitro Transcription Teaching Kit
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      Approx. Rs 13,500 / PieceGet Latest Price

      Product Brochure
      Product Details:
      Minimum Order Quantity5 Piece
      Product TypeKIT
      Country of OriginMade in India
      Number of Reactions(Preps)/Kit5xp
      BrandProgene
      Usage/ApplicationResearch and institute
      Packaging Size5xp
      Catalog NumberOP-3302-5xp

      In vitro transcription is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts are used in analytical techniques.

      CAT.NOPRODUCTEXPS
      OP-3302-5xpPro TechEx-Invitro transcription Teahing Kit5 Exps


      Components


      • DEPC treated water (-20 DegreeC)
      • Murine RNase Inhibitor (-20 DegreeC)
      • Ribonucleotide Solution Mix (-20 DegreeC)
      • SP6 RNA Polymerase (-20 DegreeC)
      • T7 RNA Polymerase Buffer (10X)(-20 DegreeC)
      • Template DNA (-20 DegreeC)
      • 1.5ml Microfuge Tubes (RT)
      • 70% Ethanol (RT)
      • Gel loading dye (RT)
      • Isopropanol (RT)
      • RNase H(-20 DegreeC)
      • Sodium acetate (3M)(RT)
      • Agarose (RT)
      • Ethidium Bormide (RT)
      • Phenol (RT)
      • T7 RNA Polymerase (-20 DegreeC)
      • TEB 10X (RT)
      • Protocol

      Additional Information:

    • Item Code: OP-3302-5xp
    • Delivery Time: 5-7 Business days
    • Yes! I am interested

      Maxi Broad Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)

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      Maxi Broad Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)
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      Approx. Rs 11,540 / PieceGet Latest Price

      Product Details:
      ModelVBR0080
      BrandOrange
      I Deal InNew Only
      TypeVertical Gel
      Dimension55(L)x20(B)x24(H)
      Number of Samples80

      Specification:
      Make : ORANGE
      Model : Regular- Maxi Broad Vertical
      Cat.No : VBR0080
      Dimension : 55(L)x20(B)x24(H)
      Number of Samples : 80
      Buffer Volume : 600 750ml
      Gel Size : 41x20cm (1 Gel)
      Glass Plate : 45x45cm Notched and Square Plates (2 Sets)
      Number of Combs : 80-wells 1mm thickness Sharktooth comb (Well Width-3mm,Interspace-2.1mm) (1 No.)
      80-wells 1.5mm thickness Sharktooth comb (Well Width-3mm,Interspace- 2.1mm) (1 No.)
      40-wells 1mm thickness Sharktooth comb (Well Width-7mm,Interspace-3mm) (1 No.)
      40-wells 1.5mm thickness Sharktooth comb (Well Width-7mm,Interspace-3mm) (1 No.)
      Spacers : 1mm and 1.5mm Spacers (3 Nos. Each)
      Gasket : Fixed
      Comb Capacity -5mm Deep Well : 15ul,22ul,35ul,52ul
      Clamp and Screws : 1 Set
      Connecting Cord : Red and Black (Each One)
      No. of Platinum electrodes : Positive and Negative (Each One)
      Buffer Chamber with Safety Lid : 1 No.
      Gel Casting System : 1 No.
      Instruction Manual 
      Warranty Card

      Additional Information:

    • Item Code: VBR0080
    • Delivery Time: 5-7 Business days
    • Yes! I am interested

       
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