Dna Extraction Kit

Approx. Rs 6,700 / KitGet Latest Price

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Random Amplified Polymorphic DNA (RAPD) is a molecular marker technique widely used in microbes and plant science. DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals, although not necessarily in a reproducible way. RAPD stands for random amplification of polymorphic DNA. It is a type of PCR reaction, but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers (8-12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns a semi- unique profile can be gleaned from a RAPD reaction. No knowledge of the DNA sequence for the targeted gene is required as the primers will bind somewhere in the sequence but it is not certain exactly where. This makes the method popular for comparing the DNA of biological systems that have not had the attention of the scientific community or in a system in which relatively few DNA sequences are compared (it is not suitable for forming a DNA databank). Due to the fact that it relies on a large intact DNA template sequence, it has some limitations in the use degraded DNA samples. Its resolving power is much lower than targeted species-specific DNA comparison methods such as short tandem repeats. In recent years RAPD is used to characterize and trace the phylogeny of diverse microbes, plant and animal species. RAPD markers offer quick screening of different regions of the genome for genetic polymorphisms.

Approx. Rs 8,500 / KitGet Latest Price

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DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome.DNA in the living cell is subject to many chemical alterations. If the genetic information encoded in the DNA is to remain uncorrupted, any chemical changes must be corrected. A failure to repair DNA produces a mutation.



Components:

• 0.2ml PCR Tubes (RT)
• 50X TAE (RT)
• Agarose (RT)
• EtBr (RT)
• Gel loading dye (RT)
• Taq Polymerase (-20°C)
• UV Damaged Lambda DNA (-20°C)
• dNTP (-20°C)Double distilled water (-20°C)
• Lambda DNA (-20°C)
• NAD+ (-20°C)
• Primer(F+R) (-20°C)
• Repair Enzyme Mix (-20°C)
• Thermopolymerase buffer (-20°C)
• Protocol