Send Email

0804764370590% Response Rate

Home

Introduction & Profiles

Our Products

Contact Us

Home » Our Products » Elisa Teaching KIts

Elisa Teaching KIts

Pioneers in the industry, we offer pro techex - dot elisa teaching kit, pro techex -sandwich elisa teaching kit, pro techex -antigen capture elisa teaching kit, pro techex -antibody capture elisa teaching kit, pro techex -direct elisa kit and pro techex - competitive elisa teaching kit from India.

Pro TechEx - Dot ELISA Teaching Kit

REQUEST CALLBACK

Get Best Quote

Approx. Rs 3,700 / KitGet Latest Price

Product Brochure

Product Details:

Minimum Order Quantity	1 Kit
Type	Direct ELISA
Brand	Progene OP-3604-15xp
Usage/Application	Laboratory
Form	Liquid
Method	Elisa
Material	Plastic

In Dot ELISA,the antigen is sandwiched directly between two antibodies which react with two different epitopes on the same antigen. Antigen in the test sample first reacts with the immobilized antibody and then with the enzyme-linked secondary antibody. The amount of enzyme linked antibody bound is assayed by incubating the strip with an appropriate chromogenic substrate, which is converted to a coloured, insoluble product. The latter precipitates onto the strip in the area of enzyme activity, hence the name Dot-ELISA. The enzyme activity is indicated by intensity of the spot, which is directly proportional to the antigen concentration.

Cat.No	Product	Experiments
OP-3604-15xp	Pro TechEx -Dot ELISA Teaching Kit	15 Exps

Components

Dot ELISA Strips (RT)

Protein-1 (4°C)

Protein-2 (4°C)

Blocker (4°C)

10X Assay Buffer (RT)

Test Serum Sample-A (4°C)

Test Serum Sample-B (4°C)

Test Serum Sample-C (4°C)

20X Antibody-HRP Conjugate (4°C)

Substrate buffer (4°C)

DAB(Chromogen) (4°C)

H2O2(Substrate) (4°C)

10X Wash Buffer (RT)

Protocol

Additional Information:

Item Code: OP-3604-15xp

Production Capacity: 100

Delivery Time: 1 Business day

Packaging Details: 1 Box- 4C

Yes! I am interested

Pro TechEx -Sandwich ELISA Teaching Kit

REQUEST CALLBACK

Get Best Quote

Approx. Rs 3,700 / KitGet Latest Price

Product Brochure

Product Details:

Minimum Order Quantity	1 Kit
Brand	Progene OP-3605-5xp
Usage/Application	Laboratory
Form	Liquid
Method	Elisa
Material	Plastic

The Sandwich ELISA measures the amount of antigen between two layers of antibodies (i.e. capture and detection antibody). The antigen to be measured must contain at least two antigenic sites capable of binding to antibody, since at least two antibodies act in the sandwich.Either monoclonal or polyclonal antibodies can be used as the capture and detection antibodies in Sandwich ELISA systems. Monoclonal antibodies recognize a single epitope that allows fine detection and quantification of small differences in antigen. A polyclonal is often used as the capture antibody to pull down as much of the antigen as possible.

CAT.NO	PRODUCT	EXPS
OP-3605-5xp	Pro TechEx -Sandwich ELISA Teaching Kit	5Exps

Components

Antigen (-20°C)
Antibody (4°C)
Coating buffer (4°C)
Blocker (4°C)
96 well plate (RT)
1X PBST (RT)
10X Wash Buffer (RT)
Antibody-HRP Conjugate (4°C)
Stop solution (4°C)
DAB(Chromogen) (4°C)
H2O2(Substrate) (4°C)
Protocol

Additional Information:

Item Code: OP-3605-5xp

Production Capacity: 100

Delivery Time: 1 Business Day

Packaging Details: 2Boxes 4C & -20C

Yes! I am interested

Pro TechEx -Antigen Capture ELISA Teaching Kit

REQUEST CALLBACK

Get Best Quote

Approx. Rs 3,700 / KitGet Latest Price

Product Brochure

Product Details:

Minimum Order Quantity	1 Kit
Type	Quantitative ELISA
Brand	PROGENE OP-3602-5xp
Form	Liquid
Method	Elisa
Features	Teaching Kit
Material	Reagents
Humidity Resistant	Yes
Country of Origin	Made in India

Antigen capture ELISA method is the most useful Immunosorbent assay for detecting antigen, since it is 2-5 folds more sensitive than those assay in which antigen is directly bound onto the solid phase. To utilize this assay, one antibody (the "capture" antibody) is purified and bound to a solid phase.Antigen is then added and allowed to complex with the bound antibody. Unbound products are then removed with a wash, and a labeled second antibody (the "detection" antibody) is allowed to bind to the antigen, thus completing the "sandwich". The assay is then quantitated by measuring the amount of labeled second antibody bound to the matrix, through the use of a colorimetric substrate.

CAT.NO	PRODUCT	EXPS
OP-3602-5xp	Pro TechEx -Antigen Capture ELISA Teaching Kit	5Exps

Components

Antibody (-20°C)
Antigen (1mg/ml stock) (-20°C)
Blocking buffer (4°C)
Coating Buffer (4°C)
Secondary Antibody( HRP- Conjugate) (-20°C)
Test Antigen (-20°C)
10X PBS (RT)
96 well plate (RT)
Stop solution (RT)
Substrate (4°C)
Wash buffer (RT)
Protocol

Additional Information:

Item Code: OP-3602-5xp

Production Capacity: 100

Delivery Time: 1 Business Day

Packaging Details: 2 Boxes-4C & -20C

Yes! I am interested

Pro TechEx -Antibody Capture ELISA Teaching Kit

REQUEST CALLBACK

Get Best Quote

Approx. Rs 3,700 / KitGet Latest Price

Product Brochure

Product Details:

Minimum Order Quantity	1 Kit
Type	Sandwich ELISA
Brand	Progene OP-3601-5xp
Usage/Application	Laboratory
Method	Elisa
Humidity Resistant	Yes
Country of Origin	Made in India

Antibody capture ELISA is a sophisticated, complex test that has proved useful in the diagnosis of many infectious diseases (e.g. rubella, measles, and toxoplasmosis). Antibody capture ELISA are particularly sensitive in demonstrating IgM responses early in illness. Reasons for this increase in sensitivity are: specific antibody isotypes in the patient''s specimen are bound to the solid phase by the capture antibody allowing the specific antibody-antigen reaction to occur in the absence of competing isotypes; soluble antigen is added in excess (allowing the antigen and antibody to react in antigen excess); and the antibody capture ELISA measures the proportion of specific to total antibody of a given isotype (which may be detectable earlier than simply measuring specific antibodies as in an ELISA test).

CAT.NO	PRODUCT	EXPS
OP-3601-5xp	Pro TechEx -Antibody Capture ELISA Teaching Kit	5Exps

Components

Antigen (-20°C)
Blocking buffer (4°C)
Test Antibody (-20°C)
Antibody (Stock) (-20°C)
Secondary Antibody( HRP- Conjugate) (-20°C)
Coating Buffer (4°C)
10X PBS (RT)
96 well plate (RT)
Stop solution (RT)
Substrate (4°C)
Wash buffer (RT)
Protocol

Additional Information:

Item Code: OP-3601-5xp

Production Capacity: 100

Delivery Time: 1 Business Day

Packaging Details: 2 Boxes - 4C & -20C

Yes! I am interested

Pro TechEx -Direct ELISA Kit

REQUEST CALLBACK

Get Best Quote

Approx. Rs 5,600 / KitGet Latest Price

Product Brochure

Product Details:

Minimum Order Quantity	1 Kit
Usage/Application	Laboratory
Sterile/ Non Sterile	Sterile
Disposable/ Reusable	Disposable
Country of Origin	Made in India
Type	Quantitative ELISA

Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labeled secondary antibody. Enzyme linked secondary antibody are added and washed. 4. A substrate is added, and enzymes on the antibody elicit a chromogenic or fluorescent signal. The Indirect-ELISA utilizes an unlabeled primary antibody in conjunction with a labeled secondary antibody. Since the labeled secondary antibody is directed against all antibodies of a given species, it can be used with a wide variety of primary antibodies.

Additional Information:

Item Code: OP-3611-10xp

Production Capacity: 100

Delivery Time: 1 Business day

Packaging Details: Two boxes 4C & -20C

Yes! I am interested

Pro TechEx - Competitive ELISA Teaching Kit

REQUEST CALLBACK

Get Best Quote

Approx. Rs 3,700 / KitGet Latest Price

Product Brochure

Product Details:

Minimum Order Quantity	1 Kit
Type	Competitive Elisa Teaching Kit
Tests Kit	Teaching Kit
Usage/Application	Laboratory
Brand	PROGENE OP-3603-5xp
Country of Origin	Made in India
Purity	99%

In this technique, antibody is first incubated in solution with a sample containing antigen. The antigen-antibody mixture is then added to an antigen coated microtiter well. The more antigen present in the sample, the less free antibody will be available to bind to the antigen-coated well. Addition of an enzyme-conjugated secondary antibody (Ab2) specific for the isotype of the primary antibody can be used to determine the amount of primary antibody bound to the well as in an indirect ELISA. In the competitive assay, however, the higher the concentration of antigen in the original sample, the lower the absorbance.