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OPRL Biosciences Pvt.,Ltd
OPRL Biosciences Pvt.,Ltd


BIOTECH KITS

Molecular Biology- Genomics:

Molecular biologyis the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas ofbiologyandchemistry, particularlygeneticsandbiochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of acell, including the interactions between the different types ofDNA,RNAandprotein biosynthesisas well as learning how these interactions are regulated.


RT-PCR Teaching Kit

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RT-PCR Teaching Kit
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Product Details:
Shelf Life12 Months
Result Time1 Day
Accuracy0.99
Usage/ApplicationLaboratory

Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike. However, they are separate and distinct techniques. While RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA, qPCR is used to quantitatively measure the amplification of DNA .

Cat. NOProductExperiments
OP-3155-5xpPro TechEx-RT-PCR Teaching Kit5 Exps

Components
RNA Template (-20°C)DNA Marker (-20°C)5x Reverse Transcriptase Buffer (-20°C)
0.2ml PCR tubes (RT)Primer F (-20°C)M-MuLV Reverse transcriptase (-20°C)
10mM DTT (-20°C)Primer R (-20°C)Ribonuclease Inhibitor (-20°C)
50X TAE (RT)Random Primer (-20°C)Taq Polymerase Buffer (-20°C)
Agarose (RT)Rnase Free Water (-20°C)Protocol
Ethidium Bromide (RT)Taq Polymerase (-20°C)
Gel Loading dye (RT)10mM dNTP (-20°C)

Additional Information:

  • Item Code: OP-3155
  • Delivery Time: 5-7 Business days
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    Chloroplast DNA Isolation Teaching Kit

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    Chloroplast DNA Isolation  Teaching Kit
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    Product Details:
    Shelf Life12 Months
    Usage/ApplicationLaboratory

    In all land plants and algae, chloroplast DNA (cpDNA) has been found to exist as a single circular molecules ranging from 80 to 300 kb. The chloroplast genome is densely packed with genes. Chloroplast gene products identifies function either in photosynthesis or as components of the chloroplast protein synthesizing system.In this procedure, sucrose step gradient procedure is used to isolate chloroplast and cpDNA.


    Cat. NO

    Product

    Experiments

    OP-3105-10xp

    Pro TechEx - Chloroplast DNA Isolation Teaching Kit

    10 Exps



    Components:

    STE Buffer (4°C)

    BSA (4°C)

    BME (4°C)

    ST Buffer (4°C)

    TE Saline Buffer (4°C)

    Isopropanol (RT)

    Tris saturated phenol (4°C)

    Phenol/Chloroform (4°C)

    Chloroform /Isoamyl alcohol (4°C)

    Magnesium acetate (RT)

    Ammonium acetate (RT)

    Dnase I (-20°C)

    Potassium acetate (RT)

    SDS (RT)

    EDTA (RT)

    Ethanol I (RT)

    Ethanol II (RT)

    Protocol


    Additional Information:

  • Item Code: OP-3105
  • Delivery Time: 5-7 Business days
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    Genomic DNA Extraction From Whole Blood Teaching Kit

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    Genomic DNA Extraction From Whole Blood Teaching Kit
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    The Genomic DNA is Isolated from Whole Blood by using RBCL solution which lysis the RBC and WBC left intact. The white blood cells and their nuclear membrane are lysis by WBCL solution then the proteins and other cellular components are removed by saturation solution. Finally the high molecular weight Genomic DNA is precipitated by using absolute alcohol. The obtained Genomic DNA is resolved by using Agarose gel electrophoresis.


    Cat. NOProduct Experiments
    OP-3125-10xpPro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit10 Exps
    OP-3125-20xpPro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit20 Exps

    Components:

    RCLB (RT)

    100% Ethanol (RT)

    50X TAE (RT)

    70% Ethanol (RT)

    Agarose (RT)

    Ethidium Bromide (RT)

    WCLB (RT)

    Gel Loading Dye (RT)

    DNA Marker (-20°C)

    Microfuge Tubes (RT)

    Saturation solution (RT)

    TE Buffer (RT)

    Protocol



    Additional Information:

  • Item Code: OP-3125
  • Delivery Time: 5-7 Business days
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    Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit

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    Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit
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    The kit contains a membrane embedded in a spin column for binding up to 1-5ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR and other molecular technique.


    Cat. NOProduct Experiments
    OP-3127-10xpPor TechEx-Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit10 Exps
    OP-3127-20xpPor TechEx-Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit 20 Exps

     

    Components:

    50X TAE (RT)

    Agarose (RT)

    column with 2ml collection

    tube (RT)

    Elution buffer (4°C)

    Ethidium bromide (RT)

    Gel loading dye (RT)

    Lysis buffer (4°C)

    TE Buffer (RT)

    Wash buffer (RT)

    Protocol



    Additional Information:

  • Item Code: OP-3127
  • Delivery Time: 5-7 Business days
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    Phage Titration Teaching Kit

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    Phage Titration Teaching Kit
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    The number of phage particles contained in the original stock phage culture is determined by counting the number of plaques formed on the seeded agar plate and multiplying this by the dilution factor. For a valid phage count, the number of plaques per plate should not exceed 300 nor be less than 30. Plates showing greater than 300 PFUs are too numerous to count (TNTC); plates showing fewer than 30 PFUs are too few to count (TFTC).

    OP-3145-5xpPro TechEx-Phage Titration Teaching Kit5 Exps
    OP-3145-20xpPro TechEx-Phage Titration Teaching Kit20 Exps


    Components:

    10X SM Buffer (RT)

    20% Maltose solution (RT)

    Agar (1.5 g /100 ml) (RT)

    Sodium Chloride (RT)

    Tryptone (RT)

    20% Magnesium Chloride (RT)

    E.coli Host (4°C)

    LB broth (RT)

    Phage lysate (4°C)

    Protocol


    Additional Information:

  • Item Code: OP-3145
  • Delivery Time: 5-7 Business days
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    Rapid Plasmid Screening Teaching Kit

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    Rapid Plasmid Screening Teaching Kit
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    A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis.

    Cat. NOProduct Experiments
    OP-3150-5xpPro TechEx-Rapid Plasmid Screening Teaching Kit5 Exps
    OP-3150-20xpPro TechEx-Rapid Plasmid Screening Teaching Kit20 Exps

    Components:

    E.coli host (4°C)

    50X TAE (RT)

    6X gel loading dye (RT)

    Agar (RT)

    Agarose (RT)

    LB-Broth (RT)

    Ampicillin (4°C)

    Ethidium Bromide (RT)

    Microfuge tube (RT)

    Solution I (4°C)

    Solution II (4°C)

    Protocol


    Additional Information:

  • Item Code: OP-3150
  • Delivery Time: 5-7 Business days
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    Genomic DNA Extraction From Bacteria Teaching Kit

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    Genomic DNA Extraction From Bacteria Teaching Kit
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    Product Details:
    BrandOrange

    Many different methods and technologies are available for the isolation of genomic DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

    CAT.NOPRODUCTEXPS
    OP-3121-10xpPro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit10 Exps
    OP-3121-20xpPro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit20 Exps
    Components

    • E.coli strain (4 DegreeC)
    • 50X TAE (RT)
    • LB media (RT)
    • Phenol/Chloroform (2524) (RT)
    • Proteinase K (4 DegreeC)
    • SDS (RT)
    • TE Buffer (RT)
    • 3M Sodium acetate pH 5.2 (RT)
    • Agarose (RT)
    • DNA Marker (-20 DegreeC)
    • Ethanol (70%) (RT)
    • Ethidium Bromide (RT)
    • Gel loading dye (RT)
    • Isopropanol (100%) (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3121-20xp
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    Long PCR Teaching Kit

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    Long PCR Teaching Kit
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    Long PCR Amplification of extremely long PCR products (up to 40 kb DNA)Long Range PCR refers to the amplification of DNA lengths that cannot typically be amplified using routine PCR methods or reagents. For simple DNA templates, polymerases optimized for Long Range PCR can amplify up to 30 kb and beyond.
    CAT.NOPRODUCTEXPS
    OP-3134-5xpPro TechEx-Long PCR Teaching Kit5 Exps
    OP-3134-20xpPro TechEx-Long PCR Teaching Kit20 Exps
    Components
    • 50X TAE (RT)
    • Agarose (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT
    • Primer(F R) (-20 DegreeC)
    • Taq Polymerase (-20 DegreeC)
    • Template DNA (-20 DegreeC)
    • 10 mM dNTP mix (-20 DegreeC)
    • 10X Taq polymerase Buffer (-20 DegreeC)
    • Distilled water (RT)
    • Marker DNA (-20 DegreeC)
    • PCR Tubes (RT)
    • Protocol

    20 Exp


    Additional Information:

  • Item Code: OP-3134-5xp
  • Delivery Time: 5-7 Business days
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    Nested PCR Teaching Kit

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    Nested PCR Teaching Kit
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    Nested PCR used to increase the specificity of DNA amplification. Two sets of primers are used in two successive reactions. In the first PCR, one pair of primers is used to generate DNA products, which may contain products amplified from non-target areas. The products from the first PCR are then used as template in a second PCR, using one ('hemi-nesting') or two different primers whose binding sites are located (nested) within the first set, thus increasing specificity. Nested PCR is often more successful in specifically amplifying long DNA products than conventional PCR, but it requires more detailed knowledge of the Sequence of the target.
    CAT.NOPRODUCTEXPS
    OP-3138-5xpPro TechEx-Nested PCR Teaching Kit5 Exps
    OP-3138-25xpPro TechEx-Nested PCR Teaching Kit25 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT)
    • Template DNA (-20 DegreeC)
    • Primer(F R) (-20 DegreeC)
    • Taq Polymerase (-20 DegreeC)
    • 10mM dNTP mix (-20 DegreeC)
    • 10X Taq polymerase Buffer (-20 DegreeC)
    • Distilled water (RT)
    • Marker DNA (-20 DegreeC)
    • PCR Tubes (RT)
    • Protocol

    25 Exp


    Additional Information:

  • Item Code: OP-3138-5Xp
  • Delivery Time: 5-7 Business days
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    Mitochondrial DNA Isolation Teaching Kit

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    Product Details:
    Shelf Life12 Months
    Accuracy0.99

    Mitochondrial DNA Isolation Teaching Kit.

    Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus, and in plants, the chloroplast as well.
    In humans, mitochondrial DNA can be assessed as the smallest chromosome coding for 37 genes and containing approximately 16,600 base pairs.Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria; cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, triphosphate (ATP).
    Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus, and in plants, the chloroplast as well.

    CAT.NOPRODUCTEXPS
    OP-3135-10xpPro TechEx-Mitochondrial DNA Isolation Teaching Kit10 Exps

    Components

    • BME(4 DegreeC)
    • BSA(4 DegreeC)
    • Isopropanol (RT)
    • ST Buffer (4 DegreeC)
    • STE Buffer (4 DegreeC)
    • TE Saline Buffer (4 DegreeC)
    • Ammonium acetate (RT)
    • Chloroform /Isoamyl alcohol (4 DegreeC)
    • DNase I (-20 DegreeC)
    • Magnesium acetate (RT)
    • Phenol/Chloroform (4 DegreeC)
    • Tris saturated phenol (4 DegreeC)
    • Ethanol II (RT)
    • EDTA (RT)
    • Ethanol I (RT)
    • SDS (RT)
    • Potassium acetate (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3135-10xp
  • Delivery Time: 5-7 Business days
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    Restriction Digestion Teaching Kit

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    Restriction Digestion Teaching Kit
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    Restriction enzymes are Nucleases which can cleave the sugar-phosphate backbone of DNA, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They specifically cleave the nucleic acids at specific nucleotide sequence called Restriction sites to generate a set of smaller fragments.

    Cat. NOProduct Experiments
    OP-3153-5xpPro TechEx-Restriction Digestion Teaching Kit5 Exps
    OP-3153-20xpPro TechEx-Restriction Digestion Teaching Kit20 Exps

    Components 
    50X TAE (RT)
    Agarose (RT)
    Ethidium bromide (RT)
    Gel loading dye (RT)
    Template DNA (-20°C)
    0.5ml tubes (RT)
    Enzyme Buffer (-20°C)
    Hind III (-20°C)
    Sterile water (RT)
    Protocol

    Additional Information:

  • Item Code: OP-3153
  • Delivery Time: 5-7 Business days
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    Transformation (Blue - White Screening) Teaching Kit

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    In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings and taken up through the cell membrane. Transformation occurs naturally in some species of bacteria, but it can also be affected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occuras a time-limited response to environmental conditions such as starvation and cell density. "Transformation" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because "transformation" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material.

    Cat. NOProduct Experiments
    OP-3164-5xpPro TechEx-Transformation (Blue - White Screening) Teaching Kit5 Exps

    Components 
    Control Transformed
    Bacteria culture (-20°C)
    Host Bacteria (-20°C)
    LB Agar (RT)
    LB broth (RT)
    Plasmid DNA (-20°C)
    Solution- A (4°C)
    1.5ml Microfuge tubes (RT)
    Ampicillin (4°C)
    Glycerol(Sterile) (4°C)
    IPTG (-20°C)
    X-gal (-20°C)
    Protocol

    Additional Information:

  • Item Code: OP-3164
  • Delivery Time: 5-7 Business days
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    Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit

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    Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit
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    Nucleosome Mapping by Micrococcal Nulease (MNase).

    The basic repeating unit of chromatin, the nucleosome, is known to play a critical role in regulating the process of gene transcription.
    The positioning of nucleosomes on a promoter is a significant determinant in its responsiveness to gene-inducing signals. For example, positioning and subsequent mobilization of nucleosomes can regulate the access of various DNA factors to underlying DNA templates.
    Several mechanisms have been proposed to direct the process of nucleosome displacement such as chemical histone modifications, ATP-dependent remodelling, and the incorporation of histone variants.
    Thus, rather than being an inert molecular structure, chromatin is highly dynamic in response to the transcription process. In this section, we describe two methodologies that allow the determination of exact nucleosome positioning within specific gene regions.

    CAT.NOPRODUCTEXPS
    OP-3139-5xpPro TechEx-Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit5 Exps
    OP-3139-20xpPro TechEx-Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit20 Exps

    Components
    • 2X TNSEK solution (RT)
    • Nuclear Buffer I (-20 DegreeC)
    • Nuclear Buffer II (-20 DegreeC)
    • Nuclear Buffer III (-20 DegreeC)
    • PBS (RT)
    • 0.1M CaCl2 (4 DegreeC)
    • MNase (-20 DegreeC)
    • NaOH 1M (RT)
    • Protocol

    MOQ-20 Exps

     


    Additional Information:

  • Item Code: OP-3139-5Xp
  • Delivery Time: 5-7 Business days
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    Transduction Teaching Kit

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    Transduction Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Transduction is the process by which DNA is transferred from one bacterium to another by a virus. It also refers to the process whereby foreign DNA is introduced into another cell via a viral vector. Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation), and it is DNase resistant (transformation is susceptible to DNase). Transduction is a common tool used by molecular biologists to stably introduce a foreign gene into a host cell's genome.

    CAT.NOPRODUCTEXPS
    OP-3163-5xpPro TechEx-Transduction Teaching Kit5 Exps
    OP-3163-20xpPro TechEx-Transduction Teaching Kit20 Exps
    Components
    • (4 DegreeC)
    • Donar strain (-20 DegreeC)
    • Host strain (-20 DegreeC)
    • (4 DegreeC)
    • Phage lysate (-20 DegreeC)
    • Recipient strain (-20 DegreeC)
    • 10% Dextrose (4 DegreeC)
    • 1M Calcium chloride (RT)
    • 1M Magnesium chloride (RT)
    • Agar (RT)
    • LB Broth (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3163-20xp
  • Delivery Time: 5-7 Business days
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    Gel Extraction Teaching Kit

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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps. This process, usually performed on plasmids.After DNA samples are run on an agarose gel, extraction involves four basic steps identifying the fragments of interest, isolating the corresponding bands, isolating the DNA from those bands, and removing the accompanying salts and stain.

    CAT.NOPRODUCTEXPS
    OP-3117-5xpPro TechEx-Gel Extraction Teaching Kit5 Exps
    OP-3117-20xpPro TechEx-Gel Extraction Teaching Kit20 Exps


    Components
    • 50X TAE (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • Silica powder (RT)
    • Solubilizing buffer (RT)
    • TE Buffer (RT)
    • Wash buffer (RT)
    • DNA sample (-20 Degree)
    • DNA marker (-20 Degree)
    • Agarose (RT)
    • Protocol


    Additional Information:

  • Item Code: OP-3117-20xp
  • Delivery Time: 5-7 Business days
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    DNA Estimation Teaching Kit

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    DNA Estimation Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Estimation of DNA is based on the Ethidium Bromide Fluorecent Staining of DNA. Ethidium Bromide is a Fluorescent dye, Which intercalates between the stacked bases. The uorescent yield of the dye - DNA complax is much graeter than the unbound dye. UV irradiation at 254nm is absorbed by the DNA and transmitted to the dye and the bounded dye itself absorbs radiation at 302nm and 366nm.

    CAT.NOPRODUCTEXPS
    OP-3111-5xpPro TechEx -DNA Estimation Teaching Kit5 Exps
    OP-3111-20xpPro TechEx -DNA Estimation Teaching Kit20 Exps

    Components
    • DNA sample-I (-20 DegreeC)
    • DNA sample-II (-20 DegreeC)
    • DNA sample-III (-20 DegreeC)
    • Standard DNA Solution (-20 DegreeC)
    • Agarose (RT)
    • Ethidium Bromide (RT)
    • Gel loading dye (RT)
    • 50X TAE buffer (RT)
    • Blank solution (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3111-20xp
  • Delivery Time: 5-7 Business days
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    Mutagenesis Teaching Kit

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    Mutagenesis Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Mutation Detection and Analysis (Mutagenesis) Teaching kit.

    Mutation occur due to deletion, insertion or duplication of bases. Mutation Can be detected by restiction enzyme mapping. Insertion, deletion or duplication of a base may create a new restiction enzyme site.

    CAT.NOPRODUCTEXPS
    OP-3137-5xpPro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit5 Exps
    OP-3137-10xpPro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit10 Exps
    Components
    • Agarose (RT)
    • Control DNA (-20 DegreeC)
    • DNA (Mutated) (-20 DegreeC)
    • Ethidium Bromide (RT)
    • Restriction Enzyme (-20 DegreeC)
    • 50X TAE (RT)
    • Gel loading Dye (RT)
    • Restriction Buffer (-20 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3137-10xp
  • Delivery Time: 5-7 Business days
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    Oligonucleotide Purification Teaching Kit

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    Oligonucleotide Purification Teaching Kit
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    Oligonucleotide synthesis involves a large number of individual reactions, which leads inevitably to the accumulation of impurities such as truncated oligonucleotides and sequences containing chemically-modified bases. These impurities must be removed after synthesis
    CAT.NOPRODUCTEXPS
    OP-3140-5xpPro TechEx-Oligonucleotide Purification Teaching Kit5 Exps
    OP-3140-10xpPro TechEx-Oligonucleotide Purification Teaching Kit10 Exps

    Components
    • 40% polyacrylamide (4 DegreeC)
    • TEMED (4 DegreeC)
    • APS (4 DegreeC)
    • Oligonucleotide I& II (-20 DegreeC)
    • Urea (RT)
    • Butanol (RT)
    • Phenol chloroform (4 DegreeC)
    • Loading buffer (4 DegreeC)
    • TE buffer (4 DegreeC)
    • Ethanol I (RT)
    • Ethanol II (RT)
    • TLC plate (RT)
    • 10X TEB (RT)
    • UV wrap (RT)
    • Protocol

    MOQ 10 Exps

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    Plasmid Purification By DNA Binding Membrane Teaching Kit

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    Plasmid Purification By DNA Binding Membrane Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationResearch and institute

    After harvesting and resuspension, the bacterial cells are lysed in NaOH-SDS in the presence of RNase A. SDS solubilizes the phospholipid and protein components of the cell membrane, leading to lysis and release of the cell contents. NaOH denatures the chromosomal and plasmid DNAs, as well as proteins. The optimized lysis time allows maximum release of plasmid DNA from the cell without release of cell wall-bound chromosomal DNA, while minimizing the exposure of the plasmid to denaturing conditions. Long exposure to alkaline conditions may cause the plasmid to become irreversibly denatured. This denatured form of the plasmid runs faster on agarose gels and is resistant to restriction enzyme digestion. The lysate is neutralized by the addition of acidic potassium acetate. The high salt concentration causes KDS* to precipitate, and the denatured proteins, chromosomal DNA, and cellular debris become trapped in salt-detergent complexes.
    CAT.NOPRODUCTEXPS
    OP-3148-10xpPro TechEx- Plasmid Purification By DNA Binding Membrane Teaching Kit10 Exps
    Components
    • 2ml Collection Vial (RT)
    • Column (RT)
    • Elution Buffer (RT)
    • Gel loading dye (RT)
    • Solution I (RT)
    • Solution II (RT)
    • Solution III (RT)
    • Wash Solution (RT)
    • 50X TAE (RT)
    • Agarose (RT)
    • Ampicillin_ (4 DegreeC)
    • DNA Marker (-20 DegreeC)
    • E.coli Host (4 DegreeC)
    • Ethidium Bromide (RT)
    • RNase A (-20 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3148-10xp
  • Delivery Time: 5-7 Business days
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    Bacterial Gene Expression Teaching Kit

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    Bacterial Gene Expression Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Bacterial Gene expression is the process by which information from a gene is used to the synthesis a functional gene product.

    CAT.NOPRODUCTEXPS
    OP-3104-5xpPro TechEx - Bacterial Gene Expression Teaching Kit5 Exps

    Components
    Recombinant bacterial culture (4 DegreeC)APS (RT)
    Inducer(IPTG) (4 DegreeC)Sample Solubilizing buffer (4 DegreeC)
    Ampicillin (4 DegreeC)Running gel buffer mix (RT)
    LB broth powder (RT)Stainer (RT)
    Separating gel mix (4 DegreeC)Destainer (RT)
    Stacking gel mix (4 DegreeC)Protocol

    Additional Information:

  • Item Code: OP-3104-5xp
  • Delivery Time: 5-7 Business days
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    Genomic DNA Extraction From Yeast Teaching Kit

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    Genomic DNA Extraction From Yeast Teaching Kit
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    Product Details:
    Shelf Life12 Months
    Accuracy0.99

    Genomic DNA Extraction From Yeast Teaching Kit.

    The Yeast Genomic DNA is isolated by Chloroform method. The cell wall is lysed by using lysis solution together with glass beads.
    It is followed by treatment with Phenol-Chloroform. Finally the DNA is precipitated by using ethanol. The obtained genomic DNA is resolved by using horizontal agarose gel electrophoresis.

    CAT.NOPRODUCTEXPS
    OP-3126-10xpPro TechEx-Genomic DNA Extraction From Yeast Teaching Kit10 Exps
    OP-3126-20xpPro TechEx-Genomic DNA Extraction From Yeast Teaching Kit20 Exps

    Components
    • Harju-Buffer(4 DegreeC)
    • Chloroform(4 DegreeC)
    • Precipitation solution(4 DegreeC)
    • Wash solution (RT)
    • RNase A (-20 DegreeC)
    • Yeast culture (4 DegreeC)
    • 50X TAE (RT)
    • Agarose (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • TE buffer (RT)
    • YPD (RT)
    • Protocol

     

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    Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit

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    Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit
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    Physical and Chemical Mutagens on E.coli teaching kit Pro Tech Ex-Study of Physical and Chemical Mutagens on The Su UV Light.

    UV light which has a lower energy content than ionizing radiations, are capable of producing lethal effects in cells exposed to low penetrating wavelengths in range of 210 to 300nm.
    Cellular components capable of absorbiong UV light are nucleic acids with the DNA as the primary site of damage. this results in the inabilityy to form viable cells after exposure to UV radiation.
    Hydroxylamine is used to mutagenize DNA in Vitro, when used in vitro, Hydroxylamine reacts with cytosine, converting it to a modified base that pairs with adenine.
    This has two consequences that is Hydroxylamine only produces GC to AT transitions, and mutations induced by hydroxylamine cannot be reverted with hydroxylamine.

    CAT.NOPRODUCTEXPS
    OP-3162-5xpPro TechEx-Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit5 Exps

    Components
    • Agar (RT)
    • E.coli host (4 DegreeC)
    • Hydroxylamine (RT)
    • LB broth (RT)
    • Saline (RT)
    • sodium hydroxide (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3162
  • Delivery Time: 5-7 Business days
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    Genomic DNA Extraction From Animal Tissue Teaching Kit

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    Genomic DNA Extraction From Animal Tissue Teaching Kit
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    Usage/ApplicationLaboratory

    DNA Extraction From Animal Tissue Teaching kit.

    A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described.
    The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS.
    The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

    CAT.NOPRODUCTEXPS
    OP-3119-10xpPro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit10 Exps
    OP-3119-20xpPro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit20 Exps

    Components
    • 50X TAE (RT)
    • Agarose (RT)
    • Chloroform (RT)
    • Ethidium Bromide (RT)
    • Gel loading Dye (RT)
    • Guanidine thiocyanate (RT)
    • 70% Ethanol (RT)
    • Isopropanol (RT)
    • RNase A (-20 DegreeC)
    • TE buffer (RT)
    • DNA Marker (-20 DegreeC)
    • Protocol

     


    Additional Information:

  • Item Code: OP-3119-10xp
  • Delivery Time: 5-7 Business days
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    Southern Blotting Teaching Kit

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    Southern Blotting Teaching Kit
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    Product Details:
    BrandOrange
    Model NumberOP-3158
    Usage/ApplicationResearch and institute

    Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1975. It allows investigators to determine the molecular weight of a restriction fragment, to measure relative amounts in different samples and to locate a particular sequence of DNA within a complex mixture. DNA (genomic or other source) is digested with a restriction enzyme and separated by gel electrophoresis and transferred from an agarose gel onto a membrane.

    CAT.NOPRODUCTEXPS
    OP-3158-5xpPro TechEx-Southern Blotting Teaching Kit5 Exps
    OP-3158-20xpPro TechEx-Southern Blotting Teaching Kit20 Exps


    Components
    • 50X TAE (RT)
    • Agarose (RT)
    • DNA sample (-20 DegreeC)
    • Ethidium Bromide (RT)
    • Gel loading dye (RT)
    • 20X SSC (RT)
    • Nitrocellulose Membrane (RT)
    • Protocol

    MOQ-20 Exps


    Additional Information:

  • Item Code: OP-3158
  • Delivery Time: 5-7 Business days
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    Study of DNA Methylation Teaching Kit

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    Study of DNA Methylation Teaching Kit
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    DNA methylation is a biochemical process where a methyl group is added to the cytosine or adenine DNA nucleotides.
    CAT.NOPRODUCTEXPS
    OP-3160-5xpPro TechEx-Study of DNA Methylation Teaching Kit5 Exps

    Components
    • EcoRI Methylase Enzyme (-20 DegreeC)
    • 10X Restriction Buffer (-20 DegreeC)
    • Lambda DNA (-20 DegreeC)
    • Methylase Buffer (10X) (-20 DegreeC)
    • Restriction enzyme (EcoRI) (-20 DegreeC)
    • S-Adenosyl methionine (SAM) (-20 DegreeC)
    • 50X TAE (RT)
    • Agarose (RT)
    • Distilled Water(-20 DegreeC)
    • Ethidium Bromide (RT)
    • Gel loading Dye (RT)
    • Microfuge Tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3160-5xp
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    DNA Molecular Weight Determination Teaching Kit

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    DNA Molecular Weight Determination Teaching Kit.

    Agarose gel electrophoresis method is used to measure the molecular size of DNA and RNA molecules. For separation of very large DNA molecules.
    The migration rates of DNA molecule depend upon their respective molecular weight.
    This simple and fast method is frequently used to determine the size of a linear DNA fragment by comparing its mobility to a DNA fragment marker of known size.

    CAT.NOPRODUCTEXPS
    OP-3116-5xpPro TechEx-DNA Molecular Weight Determination Teaching Kit5 Exps
    OP-3116-10xpPro TechEx-DNA Molecular Weight Determination Teaching Kit10 Exps


    Components
    • 50X TAE (RT)
    • DNA sample I (4 DegreeC)
    • DNA sample II (4 DegreeC)
    • DNA marker (4 DegreeC)
    • Agarose (RT)
    • Gel loading Dye (RT)
    • Ethidium Bromide (RT)
    • Protocol

    MOQ-10 Exps

     


    Additional Information:

  • Item Code: OP-3116-10xp
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    PCR Amplification of Beta Galactosidase Gene Teaching Kit

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    PCR Amplification of Beta Galactosidase Gene  Teaching Kit
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    Product Details:
    BrandOrange
    Model NumberOP-3142

    The size of pUC19 will be increased to 5755bp with the addition of lacZ gene, which encodes β-galactosidase. The lacZ gene was amplified by PCR method. The amplified sequence was transformed to E.coli host .The production of β-galactosidase enzyme was analysed by SDS - PAGE method.
    CAT.NOPRODUCTEXPS
    OP-3142-5xpPro TechEx-PCR Amplification of β-Galactosidase Gene Teaching Kit5 Exps


    Components

    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT)
    • Template DNA (-20 DegreeC)
    • Primer (-20 DegreeC)
    • Taq Polymerase (-20 DegreeC)
    • 10 mM dNTP mix (-20 DegreeC)
    • 10X Taq polymerase Buffer (-20 DegreeC)
    • Distilled water (RT)
    • Marker DNA (-20 DegreeC)
    • PCR Tubes (RT)
    • Protocol

     

    20 Exp


    Additional Information:

  • Item Code: beta-Galactosidase
  • Delivery Time: 5 to 7 business days
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    Competent Cell Preparation Teaching Kit

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    Competent Cell Preparation Teaching Kit
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    There are several ways to prepare competent cells for plasmid DNA transformation. This is the chemical method(CaCl2). Advantages are that it's simple to complete, requires no special equipment and gives good transformation efciencies.

    CAT.NOPRODUCTEXPS
    OP-3107-10xpPro TechEx -Competent Cell Preparation Teaching Kit10 Exps
    OP-3107-20xpPro TechEx -Competent Cell Preparation Teaching Kit20 Exps
    Components
    • E.coli Host (4 DegreeC)
    • Solution A (CaCl2) (4 DegreeC)
    • Agar (RT)
    • LB Broth (RT)
    • Glycerol (RT)
    • Protocol

    20 Exp

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    Southern Hybridization Teaching Kit

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    Southern Hybridization Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Hybridization involves several steps. First, the probe and sample DNA are allowed to hybridize under the appropriate conditions. The correct temperature, incubation times, and buffer conditions must be used. Next, the sample DNA is washed using conditions that will remove unhybridized probe but not the hybridized probe. Finally, the sample DNA is tested for the presence of the hybridized probe. The probe is labeled with a radioactive molecular tag (or some other tag) that allows it to be detected following hybridization.
    CAT.NOPRODUCTEXPS
    OP-3159-5xpPro TechEx-Southern Hybridization Teaching Kit5 Exps

    Components
    • 10X Safe Blue DNA Stain (-20 DegreeC)
    • 50X TAE (RT)
    • Agarose (RT)
    • Control DNA (RT)
    • DNA Sample I (RT)
    • DNA Sample II (RT)
    • EthidiumBromide(RT)
    • 10X Substrate (RT)
    • 5X Wash Buffer-A (RT)
    • 5X Wash Buffer-B (RT)
    • Biotinylated Probe (RT)
    • HRP-Conjugate (RT)
    • Hybridization Buffer (RT)
    • Prehybridization Buffer (RT)
    • 50X Electrotransfer Buffer (RT)
    • 5X Blocking Buffer (RT)
    • 5X Wash Buffer-C (RT)
    • Blocking Powder (RT)
    • Casein (RT)
    • Control Nylon Membrane (RT)
    • Nylon Membrane (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3159-5xp
  • Delivery Time: 5-7 Business days
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    Single Nucleotide Polymorphisms Teaching Kit

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    Product Details:
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    Usage/ApplicationLaboratory

    Genetic disorders and other related diseases are often associated with Single Nucleotide Polymorphism (SNP) occurring at precise positions on genes/ genomes. Development of SNP-specific primers that amplify a specific fragment in PCR when a specific SNP is present helps in disease detection.SNP detection where two DNA templates, one wild-type and the other template with SNP are used for PCR amplification individually along with 2 pairs (4 numbers) of primers, one pair specific to wild-type and the other, specific to SNP-type primer.
    CAT.NOPRODUCTEXPS
    OP-3156-5xpPro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit5 Exps
    OP-3156-20xpPro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit20 Exps
    Components

    • Control DNA (-20 DegreeC)
    • DNA Marker (-20 DegreeC)
    • dNTP Mix (-20 DegreeC)
    • Primer Mix* (-20 DegreeC)
    • SNP DNA (-20 DegreeC)
    • Taq DNA Polymerase(-20 DegreeC)
    • Taq DNA Polymerase
    • Buffer 10X (-20 DegreeC)
    • 0.2ml PCR Tube (RT)
    • 50X TAE (RT)
    • Agarose (RT)
    • Distilled water (RT)
    • Ethidium Bromide (RT)
    • Gel loading Dye (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3156
  • Delivery Time: 5-7 Business days
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    Multiplex PCR Teaching Kit

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    Multiplex PCR Teaching Kit
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    BrandOrange
    Usage/ApplicationLaboratory

    Multiplex PCR uses several pairs of primers annealing to different target sequences. This permits the simultaneous analysis of multiple targets in a single sample. For example, in testing for genetic mutations, six or more amplifications might be combined. In the standard protocol for DNA Fingerprinting, the targets assayed are often amplified in groups of 3 or 4. Multiplex Ligation-dependent Probe Amplification (or MLPA) permits multiple targets to be amplified using only a single pair of primers, avoiding the resolution limitations of multiplex PCR. Multiplex PCR has also been used for analysis of microsatellites and SNPs.
    CAT.NOPRODUCTEXPS
    OP-3136-5xpPro TechEx-Multiplex PCR Teaching Kit5 Exps
    OP-3136-25xpPro TechEx-Multiplex PCR Teaching Kit25 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT)
    • Template DNA (-20 DegreeC)
    • Multiplex Primer(F R) (-20 DegreeC)
    • Taq Polymerase (-20 DegreeC)
    • 10 mM dNTP mix (-20 DegreeC)
    • 10X Taq polymerase Buffer (-20 DegreeC)
    • Distilled water (RT)
    • Marker DNA (-20 DegreeC)
    • PCR Tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3136-25xp
  • Delivery Time: 5-7 Business days
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    PCR Application Teaching Kit

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    PCR Application Teaching Kit
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    Product Details:
    Shelf Life12 Months

    The polymerase chain reaction (PCR) has found widespread application in many areas of genetic analysis. For most, if not all of these applications, alternative methods of DNA (or RNA) amplification, can be substituted.The first application of PCR was for genetic testing, where a sample of DNA is analyzed for the presence of genetic disease mutations. Prospective parents can be tested for being genetic carriers, or their children might be tested for actually being affected by a disease. DNA samples for testing can be obtained by amniocentesis, chorionic villus sampling, or even by the analysis of rare fetal cells circulating in the mother's bloodstream. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations.

    CAT.NOPRODUCTEXPS
    OP-3143-5xpPro TechEx-PCR Application Teaching Kit5 Exps
    OP-3143-20xpPro TechEx-PCR Application Teaching Kit20 Exps

    Components
    • Control DNA I (-20°C)
    • Control DNA II (-20°C)
    • Master mix vials (-20°C)
    • Random primer (-20°C)
    • Taq polymerase (-20°C)
    • Test DNA (-20°C)
    • 1Kb DNA ladder (-20°C)
    • 50X TAE (RT)
    • Agarose (RT)
    • Ethidium bromide (RT)
    • Gel loading Dye (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3143-20xp
  • Delivery Time: 5-7 Business days
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    Isolation & Characterization of Petite Mutant Teaching Kit

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    Isolation & Characterization of Petite Mutant Teaching Kit
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    Usage/ApplicationLaboratory

    Petite Mutant teaching kit

    In this method the colonies grown on glucose medium are overlay with 2, 3, 5 Triphenyl Tetrazolium chloride (TTC). After incubation the petite colonies remains white whereas the wild type colonies turned to red because of the reduction of tetrazolium chloride resulting formazan compound.

    CAT.NOPRODUCTEXPS
    OP-3129-10xpPro TechEx-Isolation and characterization of petite mutant of Yeast Teaching Kit10 Exps

    Components
    • YPD agar (RT)
    • Saccharomyces cerevisiae (4 DegreeC)
    • Tetrazoilum Chloride (4 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3129-10Xp
  • Delivery Time: 5-7 Business days
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    PCR Teaching Kit

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    PCR  Teaching Kit
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    PCR is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the specific sequence will be accumulated in billions of copies (amplicons).

    CAT.NOPRODUCTEXPS
    OP-3144-10xpPro TechEx-PCR Teaching Kit10 Exps
    OP-3144-20xpPro TechEx-PCR Teaching Kit20 Exps

    Components

    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT)
    • Template DNA (-20°C)
    • Primer(F R) (-20°C)
    • Taq Polymerase (-20°C)
    • 10 mM dNTP mix (-20°C)
    • 10X Taq polymerase Buffer (-20°C)
    • Distilled water (RT)
    • Marker DNA (-20°C)
    • PCR Tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3144
  • Delivery Time: 5-7 Business days
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    Protoplast Fusion Teaching Kit

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    Protoplast Fusion Teaching Kit
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    An improved procedure has been developed for the fusion of plant protoplasts based on the use of polyethylene glycol possessing a low carbonyl content. Different commercial preparations of PEG were analysed using a sensitive carbonyl assay procedure and only those with a very low carbonyl content (< 5 × 10−4M) were used. Sterilisation by membrane filtration was employed, since autoclaving markedly increased the carbonyl content with associated deleterious effects on fusion frequency and heterokaryon viability. Elution of PEG was carried out using a hypotonic mannitol solution at pH 5.8, without added Ca2+, immediately followed by 9 % w/v mannitol (pH 5.8), again without added Ca2+. This improved PEG fusion procedure has been found to be applicable to a range of protoplast combinations. Its use results in a high fusion frequency with good resultant heterokaryon viability and a low level of clumping.


    Cat. NOProduct Experiments
    OP-3149-5xpProtech Ex - Protoplast fusion Teaching kit5 Exps

    Components:

    70% ethanol (RT)

    Holding solution (RT)

    Enzyme digest solution (4°C)

    Dilution solution I (RT)

    Dilution solution II (RT)

    Agglutination solution (RT)

    Glass slide (RT)

    Cover slips (RT)

    1.5ml centrifuge tubes (RT)

    Protocol


    Additional Information:

  • Item Code: OP-3149
  • Delivery Time: 5-7 Business days
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    Lambda Phage DNA Isolation Teaching Kit

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    Lambda Phage DNA Isolation Teaching Kit
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    Lambda Phage DNA Isolation Teaching Kit.

    Viruses that infect procaryotes are known as bacteriophages,or phages, because when they were first discovered,they appeared to eat bacterial cells, generating a clearing, or plaque, on a lawn of susceptible bacteria.
    In reality, the bacteria are killed by lysis as newly produced phages are released from the damaged cells.

    CAT.NOPRODUCTEXPS
    OP-3132-10xpPro TechEx-Lambda Phage DNA Isolation Teaching Kit10 Exps

    Components
    • 0.5MEDTA (RT)
    • 10% SDS (RT)
    • Ethyl alcohol (RT)
    • Phenol (RT)
    • TE Buffer (RT)
    • 0.01M MgSO4 (RT)
    • Chloroform (RT)
    • DNase I (-20 DegreeC)
    • Phenol: Chloroform (4 DegreeC)
    • RNAse (-20 DegreeC)
    • Maltose (20%) (4 DegreeC)
    • SM buffer (4 DegreeC)
    • NZY-amine medium (4 DegreeC)
    • Protocol

     


    Additional Information:

  • Item Code: OP-3132-10xp
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    Ligation Teaching Kit

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    Ligation Teaching Kit
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    Joining linear DNA fragments together with covalent bonds is called ligation. More specifically, DNA ligation involves creating a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another.The enzyme used to ligate DNA fragments is T4 DNA ligase, which originates from the T4 bacteriophage. This enzyme will ligate DNA fragments having overhanging,cohesive ends that are annealed together.

    CAT.NOPRODUCTEXPS
    OP-3133-5xpPro TechEx-Ligation Teaching Kit5 Exps
    OP-3133-20xpPro TechEx-Ligation Teaching Kit20 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • Lambda EcoR I digest (-20 DegreeC)
    • Ligase Buffer (-20 DegreeC)
    • Ligase Enzyme (-20 DegreeC)
    • Sterile water (RT)
    • 0.5ml tubes (RT)
    • Protocol

    MOQ-20 Exps

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    Restriction Analysis of Plasmid

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    Restriction Analysis of Plasmid
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    Restriction enzymes (Hind III) are Nucleases which can cleave the sugar-phosphate backbone of pBR 322 & pUC 18, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They specifically cleave the nucleic acids at specific nucleotide sequence called Restriction sites to generate a set of smaller fragments.
    CAT.NOPRODUCTEXPS
    OP-3152-5xpPro TechEx-Restriction Analysis of Plasmid (pBR322 & pUC 18) Teaching Kit5 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Gel loading Dye (RT)
    • Ethidium Bromide (RT)
    • 0.5ml Microfuge tubes (RT)
    • pBR322 (-20 DegreeC)
    • pUC18 (-20 DegreeC)
    • HindIII (-20 DegreeC)
    • Restriction Buffer (-20 DegreeC)
    • Distilled Water (-20 DegreeC)
    • Protocol

    5 Exp


    Additional Information:

  • Item Code: pBR322-pUC18
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