BIOTECH KITS
Molecular Biology- Genomics:
Molecular biologyis the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas ofbiologyandchemistry, particularlygeneticsandbiochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of acell, including the interactions between the different types ofDNA,RNAandprotein biosynthesisas well as learning how these interactions are regulated.
Pro TechEx-PCR Application Teaching Kit
Approx. Rs 6,600 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Method | PCR |
| Number of Reactions(Preps)/Kit | OP-3143-5xp |
| Brand | Progene |
| Shelf Life | 12 Months |
| Result Time (Rapid Kits) | 1 Day |
| Country of Origin | Made in India |
| Type | PCR Application Teaching Kit |
The PCR technique is based on the enzymatic replication of DNA. In PCR, a short segment of DNA is amplified using primer mediated enzymes. DNA Polymerase synthesizes new strands of DNA complementary to the template DNA. The DNA polymerase can add a nucleotide to the pre-existing 3’-OH group only. Therefore, a primer is required. Thus, more nucleotides are added to the 3’ prime end of the DNA polymerase.
In this kit the knowledge of a PCR technique and its application has been studied using multiplex PCR. Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously. The given DNA (Male/Female) was detected using multiplex target primer (SRY and ATL1 primers).
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Pro NuEx-PCR Products Purification Kit
Approx. Rs 6,000 / KitGet Latest Price
Product BrochureProduct Details:| ICMR Approved | No |
| Number of Reactions(Preps)/Kit | OP-3014-50Ex |
| Brand | Progene |
| Usage/Application | Laboratory |
| Method | PCR |
The purification method used in these protocols does not require use of phenol, chloroform,or CsCl. The DNA is purified without an additional step of ethanol precipitation.
| Cat.No | Product | Experiments |
| OP-3014-50Pr | Pro NuEx- PCR Products Purification Kit | 50 Prep |
| OP-3014-100Pr | Pro NuEx- PCR Products Purification Kit | 100 Prep |
Components:
Binding Buffer IElution BufferWash SolutionSpin Column & Collection TubeProtocol
Additional Information:
Pro TechEx-Yeast Plasmid DNA Extraction Teaching Kit
Approx. Rs 3,750 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Shelf Life | 12 Months |
| Catalog Number | OP-3166-5xp |
| Country of Origin | Made in India |
| Brand | Progene |
| Type | Yeast Plasmid DNA Extraction Teaching Kit |
Yeast Plasmid DNA Extraction Teaching Kit.
A procedure for the rapid isolation of DNA from the yeast Saccharomyces cerevisiae is described. To release plasmid DNA for the transformation of Escherichia coli , cells are subjected to vortex mixing in the presence of acid-washed glass beads, Triton X-100, sodium dodecyl sulfate, phenol and chloroform.
Centrifugation of this mixture separates the DNA from cellular debris. E. coli can be efficiently transformed with plasmid present in the aqueous layer without further purification of the plasmid DNA.
This procedure also releases chromosome DNA. Follo~ng two ethanol precipitations, the chromosomal DNA can be digested by restriction endonucleases poly A adenylation to mRNA.
| CAT.NO | PRODUCT | EXPS |
| OP-3166-5xp | Pro TechEx-Yeast Plasmid DNA Extraction Teaching Kit | 5 Exps |
| OP-3166-20xp | Pro TechEx-Yeast Plasmid DNA Extraction Teaching Kit | 20 Exps |
Components
- (4 DegreeC)
- E.coli Host (4 DegreeC)
- Cacl2 (4 DegreeC)
- Solution I (RT)
- Solution II (RT)
- Yeast Culture (4 DegreeC)
- Agar (RT)
- Glass Beads (RT)
- LB Broth (RT)
- Microfuge tubes (RT)
- YPD Broth (RT)
- Protocol
MOQ 20 Exps
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Pro TechEx-Mitochondrial DNA Isolation from Plant Teaching Kit
Approx. Rs 11,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | PROGENE |
| Temprature | RT, 4C, -20C |
| Packagint Type | Box |
| Shelf Life | 12 Months |
| Catalog Number | OP-3135-10xp |
| Result Time | 1 day |
| Product Type | KIT |
| Country of Origin | Made in India |
Mitochondria are semiautonomous organelles that manufacture cellular ATP through oxidative phosphorylation in all eukaryotes. Lynch et al. (2006) reported that plant mitochondrial genomes, which are involved in the system of energy production by encoding the necessary proteins, play a significant role in plant development and reproduction and have a specific evolutionary pattern relative to the nuclear counterparts. Isolation of plant mitochondrial DNA for use in Southern blot analysis, polymerase chain reaction (PCR) amplifications, restriction fragment length polymorphisms, and arbitrary primed DNA amplifications [amplified fragment length polymorphism (AFLP), random amplification of polymorphic DNA, simple sequence repeat-PCR] is one of the most important and time-consuming steps.
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Pro TechEx-DNA Estimation Teaching Kit
Approx. Rs 4,950 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Packaging Size | 10 |
| Catalog Number | OP-3111-10xp |
| Brand | PROGENE |
Estimation of DNA is based on the Ethidium Bromide Fluorecent Staining of DNA. Ethidium Bromide is a Fluorescent dye, Which intercalates between the stacked bases. The uorescent yield of the dye - DNA complax is much graeter than the unbound dye. UV irradiation at 254nm is absorbed by the DNA and transmitted to the dye and the bounded dye itself absorbs radiation at 302nm and 366nm.
| CAT.NO | PRODUCT | EXPS |
| OP-3111-10xp | Pro TechEx -DNA Estimation Teaching Kit | 10 Exps |
| OP-3111-20xp | Pro TechEx -DNA Estimation Teaching Kit | 20 Exps |
Components
- DNA sample-I (-20 DegreeC)
- DNA sample-II (-20 DegreeC)
- DNA sample-III (-20 DegreeC)
- Standard DNA Solution (-20 DegreeC)
- Agarose (RT)
- Ethidium Bromide (RT)
- Gel loading dye (RT)
- 50X TAE buffer (RT)
- Blank solution (RT)
- Protocol
Additional Information:
Pro TechEx- Plasmid Purification by DNA Binding Membrane Teaching Kit
Approx. Rs 3,400 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Number of Reactions(Preps)/Kit | 10xp |
| Catalog Number | OP-3148-10xp |
| Brand | Progene |
| Country of Origin | Made in India |
This kit provides a simple and efficient method for purification of up to 200µg of plasmid DNA. Bacterial cultures are lysed and the lysate are cleared by routine methods (Solution I, II, & III).Plasmid DNA from the cleared lysate is selectively adsorbed in SMS-spin column and other impurities such as proteins, salts, oligos (<40-mer) and nucleotides are washed away. Pure DNA is eluted in low-salt buffer or water. Purified Plasmid DNA can be used for any downstream applications such as sequencing, restriction reactions, labeling, transformation, PCR and Southern-blotting.
Additional Information:
Pro TechEx-Plasmid Curing Teaching Kit
Approx. Rs 3,700 / KitGet Latest Price
Product Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Grade Standard | progene |
| Catalog Number | OP-3146-10xp |
| Color | Grey |
| Brand | PROGENE |
Bacterial plasmids are known to harbor genes for: (i) resistances to antibiotics and metals, (ii) catabolic pathways such as lactose utilization and degradation of hydrocarbons (iii) biosynthesis of certain antibiotics, etc. In many cases the characteristics of the host organism conferred by the plasmids remain elusive, and such cryptic plasmids are abundant in nature. Curing of this cryptic plasmid from a bacterial strain is a method to substantiate the relationship between a genetic trait and carriage of that specific trait in the plasmid. Various methods involving chemical and physical agents have been developed to eliminate plasmids. Protocols for curing plasmids consist frequently of exposure of a culture to sub-inhibitory concentrations of some chemical agents, e.g. acridine orange, acriflavine, and sodium dodecyl sulfate or to a super-optimal temperature followed by selection of cured derivatives.
| Cat. NO | Product | Experiments |
| OP-3146-10xp | Pro TechEx-Plasmid Curing Teaching Kit | 10 Exps |
| OP-3146-20xp | Pro TechEx-Plasmid Curing Teaching Kit | 20 Exps |
Components:
E.coli host (4°C)
50X TAE (RT)
6X Gel loading dye (RT)
Agar (RT)
Agarose (RT)
LB broth (RT)
Ampicillin (4°C)
Chloroform (4°C)
Ethidium Bromide (RT)
Microfuge Tubes (RT)
Phenol (4)
Protocol
Additional Information:
Pro TechEx-Restriction Analysis of Plasmid (pBR322 & pUC18) Teaching Kit
Approx. Rs 8,400 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Packagint Type | Box |
| Packaging Size | 5xp |
| Grade Standard | Progene |
| Catalog Number | OP-3152-5xp |
| Shelf Life | 12 Months |
| Country of Origin | Made in India |
Restriction enzymes hydrolyze the backbone of DNA between deoxyribose and phosphate groups. This cleaves a phosphate group on the 5’ ends and a hydroxyl group on the 3’ ends of both strands. The restriction enzymes mostly used in molecular biology labs, cut within the recognition sites and generate two different types of ends. The 5’ or 3’ overhangs generated by enzymes cut asymmetrically that are called sticky or cohesive ends, because they will readily stick or anneal with their complementary sequences by base pairing E.g.: EcoRI. Some enzymes cut precisely at opposite sites in two strands of DNA and generate blunt ends without overhangs called blunt ends. E.g.: Hae III.
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Pro TechEx-Reporter Gene Assay Teaching Kit
Approx. Rs 10,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | OP-3151-5xp |
| Packagint Type | Box |
| Catalog Number | OP-3151-5xp |
| Shelf Life | 12 Months |
| Humidity Resistant | Yes |
| Country of Origin | Made in India |
The blue/white screening method works by disrupting this α complementation process. The plasmid carries within the lacZα sequence an internal multiple cloning site (MCS). This MCS within the lacZα sequence can be cut by restriction enzymes so that the foreign DNA may be inserted within the lacZα gene, thereby disrupting the gene that produces α-peptide. Consequently, in cells containing the plasmid with an insert, no functional β galactosidase may be formed. The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β-galactosidase to form 5 bromo-4-chloro-indoxyl, which then spontaneously dimerizes and oxidizes to form a bright blue insoluble pigment 5,5'-dibromo-4,4'-dichloro-indigo. This results in a characteristic blue colour in cells containing a functional β 3 galactosidase. Blue colonies therefore show that they may contain a vector with an uninterrupted lacZα (therefore no insert), while white colonies, where X-gal is not hydrolyzed, indicate the presence of an insert in lacZα which disrupts the formation of an active β-galactosidase. The recombinant clones can be further analyzed by isolating and purifying small amounts of plasmid DNA from the transformed colonies and restriction enzymes can be used to cut the clone and determine if it has the fragment of interest.
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Pro TechEx-Restriction Mapping Teaching Kit
Approx. Rs 4,250 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Packaging Size | 5xp |
| Number of Reactions(Preps)/Kit | 5xp |
| Catalog Number | OP-3154-5xp |
| Product Type | KIT |
| Brand | Progene |
| Country of Origin | Made in India |
Restriction enzymes hydrolyze the backbone of DNA between deoxyribose and phosphate groups. This cleaves a phosphate group on the 5’ ends and a hydroxyl group on the 3’ ends of both strands. The restriction enzymes mostly used in molecular biology labs, cut within the recognition sites and generate two different types of ends. The 5’ or 3’ overhangs generated by enzymes cut asymmetrically that are called sticky or cohesive ends, because they will readily stick or anneal with their complementary sequences by base pairing E.g.: EcoRI. Some enzymes cut precisely at opposite sites in two strands of DNA and generate blunt ends without overhangs called blunt ends. E.g.: Hae III.
Additional Information:
Pro TechEx-Southern Hybridization Teaching Kit
Approx. Rs 9,000 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Packaging Size | 5 |
| Catalog Number | OP-3159-5xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
Hybridization involves several steps. First, the probe and sample DNA are allowed to hybridize under the appropriate conditions. The correct temperature, incubation times, and buffer conditions must be used. Next, the sample DNA is washed using conditions that will remove unhybridized probe but not the hybridized probe. Finally, the sample DNA is tested for the presence of the hybridized probe. The probe is labeled with a radioactive molecular tag (or some other tag) that allows it to be detected following hybridization.
Additional Information:
Pro TechEx-Optimization of time of Inducer Concentration on E.coli Recombinant Protein Expression
Approx. Rs 8,300 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Shelf Life | 12 Months |
| Catalog Number | OP-3141-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
| Type | Optimization of time of Inducer Concentration on E.coli Recombinant Protein Expression Teaching Kit |
E.coli cells are grown along with inducer (IPTG) at different time intervals. After the incubation period the cells were centrifuged and cell lysate were prepared. The cell lysate were used to assay for E.coli recombinant protein expression. β-Galactosidase is an enzyme that catalyzes the hydrolysis of βgalactosides, including lactose and the galactoside analog o-nitrophenylβ-D-galactopyranoside (ONPG). Cell lysates are simply incubated with a reaction buffer and ONPG substrate. β-Galactosidase converts the colorless ONPG substrate into galactose and the Chromophore o-nitrophenol, yielding a bright yellow solution. The β-galactosidase activity of the solution can be quantitated 2 using a spectrophotometer to determine the amount of substrate converted at 420 nm. The β-Galactosidase Assay Kit is excellent for determining enzyme activity in lysates from cells transfected with a βgalactosidase expression construct.
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Pro TechEx- DNA Silver Staining Teaching Kit
Approx. Rs 4,300 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Packagint Type | Box |
| Packaging Size | OP-3222D-10xp |
| Grade Standard | Progene |
| Physical State | Liquid |
| Catalog Number | OP-3222D-10xp |
| Result Time | 1 day |
| Shelf Life | 6 Months |
| Country of Origin | Made in India |
This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis (PAGE). The minimum 0.44 and 3.5 ng of DNA amount could be detected in denaturing and nondenaturing polyacrylamide gel, respectively.
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Pro TechEx-Transformation Of Yeast Cell Using Lithium Acetate Teaching Kit
Approx. Rs 5,400 / KitGet Latest Price
Product BrochureProduct Details:| ICMR Approved | No |
| Brand | PROGENE |
| Usage/Application | Laboratory |
| Form | Liquid |
| Catalog Number | OP-3165-10xp |
| Material | Plastic |
| Shelf Life | 12 Months |
| Humidity Resistant | Yes |
This kit describes a simple and efficient procedure for transformation of yeast cells. Colonies grown on minimal (SD) media were directly removed and suspended in a reaction mixture containing TEL buffer (PEG-4000, lithium acetate, tris- HCL-pH4.9 and EDTA) plasmid DNA, carrier DNA and sterile water. After incubation at 30°C for 1hr followed by heat shock at 42°C for 15mins. The transformation efficiency obtained using the procedure was approximately 8000 transformants/µg. The method is easy and time saving.
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Pro TechEx-Competent Cell Preparation Teaching Kit
Approx. Rs 6,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Kit |
| ICMR Approved | No |
| Brand | Progene |
| Packaging Size | 10 |
| Catalog Number | OP-3107-10xp |
| Product Type | KIT |
| Country of Origin | Made in India |
Competent cells are ready to use bacterial cells that possess more easily altered cell walls by which foreign DNA can be passed through easily. Most types of cells cannot take up DNA efficiently unless they have been exposed to special chemical or electrical treatments to make them competent. The standard method for making the bacteria permeable to DNA involves treatment with calcium ions. Brief exposure of cells to an electric field also allows the bacteria to take up DNA and this process is called as electroporation. However, some types of bacteria are naturally transformable, which means they can take up DNA from their environment without requiring special treatment. The exact mechanisms involved in artificial competence are not yet known well. In CaCl2 method, the competency can be obtained by creating pores in bacterial cells by suspending them in a solution containing high concentration of calcium. DNA can then forced in to the Host cell by heat shock treatment at 42oC for the process of transformation.
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Pro TechEx-Site Directed Mutagenesis by PCR Teaching Kit
Approx. Rs 6,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | PROGENE |
| Form | Liquid |
| Catalog Number | OP-3157-5xp |
| Shelf Life | 12 Months |
| Humidity Resistant | Yes |
| Country of Origin | Made in India |
The key to the Site Directed Mutagenesis technique is that the fully mutated, fully transformable plasmid is exponentially amplified while hemi mutated plasmid is linearly amplified and non-mutated plasmid is not amplified at all. 3 As the first cycle progresses, the double-stranded template plasmid is denature into two single-stranded circular DNA molecules. As the temperature decreases, primer anneals to the circular ssDNA and the polymerase elongates from the 3'-end of the primer around the circle until the nascent DNA strand about the 5'-end of the primer, thus creating a dsDNA plasmid with the newly polymerized strand bearing a gap in the DNA. Thermal stable ligase seals the gap creating a replicated, double-stranded plasmid DNA. Note that in this round, the base change encoded by the mutagenic primer is incorporated into only one of the two DNA strands of only one of the two replicated plasmids. During the second round of PCR the two product plasmids are denatured resulting in four ssDNA closed circles, one of which bears the desired mutation. The primers anneal and extension and ligation ensue. The products from the second round of PCR amplification are one fully mutated plasmid, two hemi-mutated plasmids, and one unmutated plasmid. Cycle 2 recapitulates the products present at the end of cycle. Each of these plasmids undergoes denaturation, annealing, extension, and ligation (Non-Enzymatic template directed ligation reaction of triphosphate activated oligonucleotide), resulting in eight plasmids, four of which are fully mutated, three are hemi-mutated and one is unmutated. Cycle 4, depicts the population of products present at the end of the fourth round of PCR amplification: eleven fully mutated plasmid molecules, 5 hemi-mutated molecules, and a single unmutated plasmid molecule. After 10 cycles the ratio of mutated to unmutated plasmid is 1,000:1, and that of mutated to hemi mutated plasmid 100:1. However, after 20 cycles these ratios are 1,000,000:1 and 50,000:1. Of course, this is an idealized representation of a complex process and assumes a PCR efficiency of 100%. After 5 to 25 PCR cycles Hind III digestion of the parental plasmid, population composed of over 70 to 90% fully mutated plasmids in control reactions.
Additional Information:
Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit
Approx. Rs 5,200 / KitGet Latest Price
Product Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Packaging Size | 5 |
| Catalog Number | OP-3137-5xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
Mutation Detection and Analysis (Mutagenesis) Teaching kit.
Mutation occur due to deletion, insertion or duplication of bases. Mutation Can be detected by restiction enzyme mapping. Insertion, deletion or duplication of a base may create a new restiction enzyme site.
| CAT.NO | PRODUCT | EXPS |
| OP-3137-5xp | Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit | 5 Exps |
| OP-3137-10xp | Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit | 10 Exps |
- Agarose (RT)
- Control DNA (-20 DegreeC)
- DNA (Mutated) (-20 DegreeC)
- Ethidium Bromide (RT)
- Restriction Enzyme (-20 DegreeC)
- 50X TAE (RT)
- Gel loading Dye (RT)
- Restriction Buffer (-20 DegreeC)
- Protocol
Additional Information:
Pro TechEx-Isolation and characterization of petite mutant of Yeast Teaching Kit
Approx. Rs 5,000 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Usage/Application | Laboratory |
| Packaging Size | 10 |
| Number of Reactions(Preps)/Kit | 10 |
| Catalog Number | OP-3129-10xp |
| Product Type | KIT |
| Brand | PROGENE |
| Country of Origin | Made in India |
Yeast Saccharomyces cerevisiae cells are grown and petite mutant are induced with ethidium bromide which is an inhibitor of mitochondria. The plates are spread with treated and untreated cells of Yeast. The growth differentiation was absorbed by spraying 2,3,5-Triphenyltetrazolium chloride (TTC) is a colorless water-soluble dye that is reduced by the mitochondrial enzyme succinate dehydrogenase of living cells into a water-insoluble, light sensitive compound (formazan) that turns healthy/normal tissue deep red.
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