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OPRL Biosciences Pvt.,Ltd
OPRL Biosciences Pvt.,Ltd


BIO TECH KITS

It contains two types of Method :

* DNA & RNA Extraction By Spin Column Method

* DNA & RNA Extraction By Reagent Method

Ultra Pure Plasmid DNA Extraction Kit

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Ultra Pure Plasmid DNA Extraction Kit
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Product Details:
Shelf Life12 Months
Accuracy0.99

Plasmid DNA Mini Preparation provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/ chloroform extraction. It is based on binding of DNA to silica-based membranes in a chaotropic salt. An average yield of 1-10 ug plasmid DNA can be expected from 1-3 ml overnight bacterial culture.

Cat.NoProductPreparation
OP-3015-50PrPro NuEx-Ultrapure Plasmid DNA Extraction Kit50Prep
OP-3015-100PrPro NuEx-Ultrapure Plasmid DNA Extraction Kit100Prep

Components50 Preparation 100 Preparation
Elution Buffer5ml10ml
RNAse A120µl240µl
Solution I6ml12ml
Solution II12ml24ml
Solution III25ml50ml
Wash Solution12ml24ml
Spin Column & Colletion Tube50Nos100Nos
ProtocolOneOne

Additional Information:

  • Item Code: OP-3015
  • Delivery Time: 5-7 Business days
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    Total RNA Extraction Kit

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    Total RNA Extraction Kit
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    RNA Extraction System provides an method to purify total RNA from various samples such as cultured cells, tissues, bacteria, and yeast. A simple silica-membrane spin-column method can isolate total RNA without phenol/ chloroform extraction and ethanol precipitation. Total RNA with a molecular size greater than 200 nucleotides .

    Cat.NoProductPreparation
    OP-3016-10PrPro NuEx-Total RNA extraction Kit10Prep
    OP-3016-20PrPro NuEx-Total RNA extraction Kit20Prep

    Components10 Preparation20 Preparation
    Solution G17ml14ml
    Solution G26ml12ml
    Solution G32.5ml5ml
    DEPC Treated Water0.5ml1ml
    Spin Column & Colletion Tube10Nos20Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3016
  • Delivery Time: 5-7 Business days
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    Bacterial Genomic DNA Extraction Kit

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    Bacterial Genomic DNA Extraction Kit
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    Quick one step method enables rapid isolation of Genomic DNA from Bacteria .
    Cat.NoProductExtraction
    OP-3018-1mlPro NuEx-Bacterial Genomic DNA Extraction Kit1ml
    OP-3018-5mlPro NuEx-Bacterial Genomic DNA Extraction Kit1mlX5Nos

    Additional Information:

  • Item Code: OP-3018
  • Delivery Time: 5-7 Business days
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    Whole Blood Genomic DNA Extraction Kit (From Fresh & Frozen Blood)

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    Whole Blood Genomic DNA Extraction Kit (From Fresh & Frozen Blood)
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    This kit is used for rapid isolation of genomic DNA from fresh and frozen blood sample.

    Cat.NoProductExtraction
    OP-3024-50ExPro NuEx-Whole Blood Genomic DNA Extraction Kit (From Fresh and Frozen Blood)50Ext
    Components50 Extraction
    Cell Lysis Buffer125ml
    DNA extraction Buffer15ml
    Ethanol30ml
    TE Buffer1ml
    ProtocolOne


    Additional Information:

  • Item Code: OP-3024-50EX
  • Delivery Time: 5-7 Business days
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    Ultrapure DNA Extraction from Soil Sample

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    Ultrapure DNA Extraction from Soil Sample
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    DNA Extraction Kit provides all the reagents necessary to recover PCR ready DNA from a variety of environmental samples. The kit utilizes a hot detergent lysis process.

    Cat.NoProductExtraction
    OP-3001-50ExPro NuEx-Ultrapure DNA Extraction from Soil Sample50 Ext
    OP-3001-100Ex
    Pro NuEx-Ultrapure DNA Extraction from Soil Sample


    100 Ext




    Components: 50 Ext
    Digestion Solution - 50 ml
    Elution Buffer - 5ml
    Soil Lysis solution - 100ml
    Proteinase K -150ul
    Solution A - 50ml
    Spin column & collection tube - 50 NOs
    Pellet wash solution - 12ml
    Protocol - One

    Components: 100 Ext
    Digestion Solution - 100 ml
    Elution Buffer - 10ml
    Soil Lysis solution - 200ml
    Proteinase K -300ul
    Solution A - 100ml
    Spin column & collection tube - 100 NOs
    Pellet wash solution - 22ml
    Protocol - One

    Additional Information:

  • Item Code: OP-3001-50Ex
  • Delivery Time: 5-7 Business days
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    Ultrapure DNA Gel Extraction Kit

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    Ultrapure DNA Gel Extraction Kit
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    Product Details:
    Accuracy0.99

    The Kit provide a simple and efficient method for DNA extraction from agarose gel.The sample is mixed with binding buffer and in case of a cut-out gel band, It is heated to dissolve the agarose.in the presence of chaotrophic salt, The dna is the bound to the silica membrane of spin column.contaminations are removed by sinple washing steps with ethanolic wash buffer. Finally, the pure DNA is eluted under low salt conditions with slightly alkaline elution buffer (2mMTris/HCL, pH 8.5)
    Cat.NoProductExtraction
    OP-3002-50ExPro NuEx-Ultrapure DNA Gel Extraction Kit50 Ext
    OP-3002-100ExPro NuEx-Ultrapure DNA Gel Extraction Kit100 Ext


    Components: 50Ext
    Binding Buffer -30 ml
    Elution Buffer -5ml
    Spin column & Collection Tube -50Nos
    Wash Buffer - 12ml
    Protocol - 1

    Components: 100Ext
    Binding Buffer -60 ml
    Elution Buffer -10ml
    Spin column & Collection Tube -100Nos
    Wash Buffer - 24ml
    Protocol - 1

    Additional Information:

  • Item Code: OP-3002-50Ex
  • Delivery Time: 5-7 Business days
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    Bacterial Protein Extraction Kit

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    Bacterial Protein Extraction Kit
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    Bacterial protein extraction kit is designed for the lysis of bacterial cells for the purification of recombinant type proteins. It is useful for the preparation of high inclusion bodies. This method does not require special equipment’s such as sonicater or French press.


    Cat.No

    Product

    Extraction

    OP-3019-1L

    Pro NuEx-Bacterial Protein Extraction Kit

    For 1Ltr Culture medium

    OP-3019-5L

    Pro NuEx-Bacterial Protein Extraction Kit

    For 5Ltr Culture medium


    Components:

    10X cell lysis buffer

    2X SDS Gel loading buffer

    DNAse & RNase

    Lysozyme

    PMSF

    Protocol


    Additional Information:

  • Item Code: OP-3019-1L
  • Delivery Time: 5-7 Business days
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    Isolation of Genomic DNA from Fish Kit

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    Isolation of Genomic DNA from Fish Kit
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    This kit is used for rapid isolation of genomic DNA from Fish and Fins sample

    Cat.No

    Product

    Extraction

     

    OP-3025-25Ex

     

    Pro NuEx-Isolation of Genomic DNA from Fish kit 25 Ext

     

    25 Ext

     

    OP-3025-50Ex

     

    Pro NuEx-Isolation of Genomic DNA from Fish kit 50 Ext

     

    50 Ext

     

    Components:

    Extraction buffer

    Solution I

    Chloroform: Isoamylalcohol

    Sodium acetate

    Ethanol

    Wash buffer

    Agarose

    Microfuge tube

    TE buffer

    RNaseA

    Ethidium Bromide

    Gel loading dye

    50XTAE

    Protocol

     


    Additional Information:

  • Item Code: OP-3025
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction from Tissue

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    Ultrapure Genomic DNA Extraction from Tissue
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    The kit is designed for fast extraction and purification of DNA from tissue sample. The kit is provided with spin column can bind up to 10μg of puried DNA.

    Cat.NoProductExtraction
    OP-3011-50ExPro NuEx-Genomic DNA Extraction from Tissue Kit50Ext
    OP-3011-100ExPro NuEx-Genomic DNA Extraction from Tissue Kit100Ext

    Components50 Extraction100 Extraction
    ACL Solution20ml40ml
    AB solution20ml40ml
    PBS20ml40ml
    Proteinase K1ml2ml
    Elution Buffer5ml10ml
    wash Solution12ml24ml
    Spin Column & Collection Tube50Nos100Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3011
  • Delivery Time: 5-7 Business days
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    Ultra Pure Genomic DNA Extraction From Hair

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    Ultra Pure Genomic DNA Extraction From Hair
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    Product Details:
    Usage/ApplicationLaboratory
    Shelf Life12 Months
    Accuracy0.99

    Minimum quantity of hair sample is enough to perform the extraction. The kit is designed for fast extraction and purification of DNA from hair sample. The kit is provided with spin column can bind up to 10μg of purified DNA. Other impurities like proteins,salts can not bind to the column.

    Cat.NoProductExtraction
    OP-3007-50ExPro NuEx-Ultrapure Genomic DNA Extraction from Hair50 Ext
    OP-3007-100ExPro NuEx-Ultrapure Genomic DNA Extraction from Hair100 Ext


    Components
    Binding Buffer
    DTT
    Elution Buffer
    Extraction Buffer
    Proteinase K
    Wash Buffer
    Spin Column & Collection Tube
    Protocol

    Additional Information:

  • Item Code: OP-3007
  • Delivery Time: 5-7 Business days
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    Mammalian Protein Extraction Kit

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    Mammalian Protein Extraction Kit
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    It is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications.

    Cat.No Product Experiments 
    OP-3020-10 PrPro NuEx-Mammalian Protein Extraction Kit10 Prep


    Components
    Extraction Buffer
    PMSF
    Protease inhibitor
    Protocol

    Additional Information:

  • Item Code: OP-3020-10Pr
  • Delivery Time: 5-7 Business days
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    PCR Products Purification Kit

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    PCR Products Purification Kit
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    The purification method used in these protocols does not require use of phenol, chloroform,or CsCl. The DNA is purified without an additional step of ethanol precipitation.

    Cat.No Product Experiments 
    OP-3014-50PrPro NuEx- PCR Products Purification Kit50 Prep
    OP-3014-100PrPro NuEx- PCR Products Purification Kit100 Prep


    Components:

    Binding Buffer IElution BufferWash SolutionSpin Column & Collection TubeProtocol

    Additional Information:

  • Item Code: OP-3014
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction From Bone

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    Ultrapure Genomic DNA Extraction From Bone
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    The kit is designed for fast extraction and purification of DNA from bone. The spin column provided have the ability to bind up to 5-10μg of DNA only.

    Cat.No Product Experiments 
    OP-3005-50ExPro NuEx-Ultrapure Genomic DNA Extraction from Bone50 Exps
    OP-3005-100ExPro NuEx-Ultrapure Genomic DNA Extraction from Bone100 Exps


    Components:

    Binding BufferElution BufferExtraction BufferProteinase KWash BufferSpin Column & Collection TubeProtocol

    Additional Information:

  • Item Code: OP-3005
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction from Frozen Tissue

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    Ultrapure Genomic DNA Extraction from Frozen Tissue
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    The kit is designed for fast extraction and purification of DNA from tissue sample. The kit is provided with spin column can bind up to 10μg of purified DNA. Other impurities like proteins,salts can not bind to the column.

    Cat.No Product Experiments 
    OP-3006-50ExPro NuEx-Ultrapure Genomic DNA Extraction from Frozen Tissue50 Ext
    OP-3006-100ExPro NuEx-Ultrapure Genomic DNA Extraction from Frozen Tissue100 Ext


    Components:

    AB solutionATL SolutionElution BufferPBSProteinase Kwash SolutionSpin Column & Collection TubeProtocol

    Additional Information:

  • Item Code: OP-3006
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction From Plant

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    Ultrapure Genomic DNA Extraction From Plant
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    The kit is designed for fast extraction and purification of DNA from plant tissue sample. The kit is provided with spin column can bind up to 10μg of purified DNA. Other impurities like proteins,salts can not bind to the column.

    Cat.No Product Experiments 
    OP-3009-50ExPro NuEx-Ultrapure Genomic DNA Extraction from Plant50 Ext
    OP-3009-100ExPro NuEx-Ultrapure Genomic DNA Extraction from Plant100 Ext


    Components:

    Elution BufferRNase ASolution A1Solution A2Solution A3Wash SolutionSpin Column & Collection TubeProtocol

    Additional Information:

  • Item Code: OP-3009
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction from Plant (CTAB Method)

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    Ultrapure Genomic DNA Extraction from Plant (CTAB Method)
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    Homogenised plant tissue is suspended in genomic DNA extraction buffer containing CTAB. Cells are lysed by osmotic shock and cell debris is precipitated and removed by using phenol chloroform and followed by ethanol precipitation of DNA.

    Cat.NoProductExperiments
    OP-3010-10ExPro NuEx-Ultrapure Genomic DNA Extraction from Plant (CTAB Method)10 Ext
    OP-3010-50ExPro NuEx-Ultrapure Genomic DNA Extraction from Plant (CTAB Method)50 Ext


    Components:

    Solution I
    Solution II
    Solution III
    Precipitation Solution
    Proteinase K
    RNase
    ATE Buffer
    Protocol

    Additional Information:

  • Item Code: OP-3010
  • Delivery Time: 5-7 Business days
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    PCR Cleanup Extraction Kit

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    PCR Cleanup Extraction Kit
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    Product Details:
    Accuracy0.99

    The Spin Column Kits provide a simple and efficient method for extraction of DNA from agarose gels, and purification of DNA from enzymati creations such as PCR or restriction enzyme digestions. The DNA is selectively adsorbed in silica gel-based column and other components are washed away. The DNA is then eluted off the column and can be used for any downstream applications.Cat.No Product Preparation

    Cat.NoProductPreparation
    OP-3013-50PrPro NuEx-PCR Cleanup Extraction Kit50Prep
    OP-3013-100PrPro NuEx-PCR Cleanup Extraction Kit100Prep

    Components50Preparation100Preparation
    Cleanup Solution20ml40ml
    Elution Buffer5ml10ml
    Wash Solution12ml24ml
    Spin Column & Collection Tube50Nos100Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3013
  • Delivery Time: 5-7 Business days
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    Bacterial Cell Lysis Reagent Kit

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    Bacterial Cell Lysis Reagent Kit
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    Cell lysis solutions are detergent-based, buffers and reagent sets that have been optimized for particular cell lysis applications. Effective cell lysis and protein extraction for different species of organisms and different cell and tissue types require different buffer formulations.




    Cat.No

    Product

    Extraction

    OP-3017-1ml

    Pro NuEx-Bacterial Cell lysis reagent Kit

    1 ml

    OP-3017-5ml

    Pro NuEx-Bacterial Cell lysis reagent Kit

    1ml x 5 Nos


    Additional Information:

  • Item Code: OP-3017
  • Delivery Time: 5-7 Business days
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    Plant DNA Extraction Kit (For PCR Amplification)

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    Plant DNA Extraction Kit (For PCR Amplification)
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    Product Details:
    Shelf Life12 Months
    Accuracy0.99

    This kit is designed for quick one step method for DNA extraction from plant leaf. This isolated DNA can be directly used to perform PCR.

    Cat.NoProductExtraction
    OP-3021-10ExPro NuEx-Plant DNA Extraction Kit (For PCR Amplification)100Ext

    Components100 Extraction
    DNA extraction Solution100ml
    ProtocolOne

    Additional Information:

  • Item Code: OP-3021-100Ex
  • Delivery Time: 5-7 Business days
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    Ultra Pure Genomic DNA Extraction from Bacteria

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    Ultra Pure Genomic DNA Extraction from Bacteria
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    Product Details:
    Shelf Life12 Months
    Accuracy0.99

    Genomic DNA Extraction Kit (for Bacteria) is spin type product to extract almost all of Gram (-)/( ) Bacteria. Simple & Fast: It takes only 30 ~ 40 minutes to extract genomic DNA Spin Column type: Extract genomic DNA without organic extraction or ethanol precipitation. High purity & yield of genomic DNA can be up to 10µg.

    Cat.NoProductExtraction
    OP-3003-50ExPro NuEx-Genomic DNA Extraction from Bacteria Kit50Ext
    OP-3003-100ExPro NuEx-Genomic DNA Extraction from Bacteria Kit100Ext

    Components50Extraction100Extraction
    Digestion Solution20ml40ml
    Elution buffer5ml10ml
    Proteinase K150µl300µl
    Wash Buffer12ml24ml
    Spin Column & Collection Tube50Nos100Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3003
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    Single Step Ultra-Competetent Cell Preparation Kit

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    Single Step Ultra-Competetent Cell Preparation Kit
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    Product Details:
    BrandOrange
    ApplicationLaboratory

    This kit contain SSCS solution, enables research to prepare competent E.coli cells in a single step and to transform cells with out heat-shock treatment.

    Cat. NOProductExperiments
    OP-3022-25mlPro NuEx-Single Step Ultra-competetent cell preparation Kit25ml
    OP-3022-50mlPro NuEx-Single Step Ultra-competetent cell preparation Kit50ml


    Components25ml50ml
    SSCS solution25ml50ml
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3022
  • Delivery Time: 5-7 Business days
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    mRNA Purification Kit

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    mRNA Purification  Kit
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    Product Details:
    Accuracy0.99

    mRNA Isolation Kit is designed to isolate intact poly(A) RNA from cells and tissue without requiring phenol or other organic solvents. the kit contain guanidine-thiocyanate based buffers for efficient cell and tissue lysis,protein denaturation and Rnase inactivation to keep the mRNAintact. The technology is based on the coupling of Oligo d(T)25 to 1 m paramagnetic beads which is then used as the solid support for the direct binding of poly(A) RNA.

    Cat.NoProductPreparation
    OP-3012-10prPro NuEx-mRNA Purification Kit10Prep
    OP-3012-20prPro NuEx-mRNA Purification Kit20Prep

    Components10 Preparation20 Preparation
    Digestion Solution10ml20ml
    Oligo dT Cellulose10X0.5mg20X0.5mg
    R-Elution Buffer15ml30ml
    RNA binding buffer15ml30ml
    Sodium acetate pH42ml4ml
    Spin Column & Collection Tube10Nos20Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3012
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction from Blood

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    Ultrapure Genomic DNA Extraction from Blood
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    Product Details:
    Shelf Life12 Months
    Accuracy0.99

    The kit used for fast extraction and purification of genomic DNA from clinical samples such as fresh or frozen whole blood,serum and other body fluids. 300µl of sample per spin column is enough to obtain up to 10µg of pure genomic DNA.

    Cat.NoProductExtraction
    OP-3004-50ExPro NuEx-Genomic DNA Extraction from Blood Kit50Ext
    OP-3004-100ExPro NuEx-Genomic DNA Extraction from Blood Kit100Ext

    Components50 Extraction 100Extraction 
    B1 Buffer120ml2x120ml
    B2 Buffer25ml50ml
    Elution Buffer5ml10ml
    Proteinase K150µl300µl
    TE Buffer15ml30ml
    Wash Solution12ml24ml
    Spin Column & Collection Tube50Nos100Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3004
  • Delivery Time: 5-7 Business days
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    Ultrapure Genomic DNA Extraction From Nail

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    Ultrapure Genomic DNA Extraction From Nail
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    Product Details:
    Shelf Life12 Months
    Accuracy0.99

    fication of DNA from Nail sample. The kit is provided with spin column can bind up to 10ed DNA.

    Cat.NoProductExtraction
    OP-3008-50ExPro NuEx-Genomic DNA Extraction from Nail Kit50Ext
    OP-3008-100ExPro NuEx-Genomic DNA Extraction from Nail Kit100Ext


    Components50Extraction100Extraction
    Binding Buffer12.5ml25ml
    DTT500l1ml
    Elution Buffer5ml10ml
    Extraction Buffer10ml20ml
    Proteinase K150l300l
    Wash Buffer12.5ml25ml
    Spin Column & Collection Tube50Nos100Nos
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3008
  • Delivery Time: 5-7 Business days
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    Total RNA Extraction Kit ( By Trizol)

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    Total RNA Extraction Kit ( By Trizol)
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    Total RNA Extraction (Trizol)is ready to usereagent for the isolation of total RNA from cells and tissues.

    Cat.NoProductExtraction
    OP-3023-25mlPro NuEx-Total RNA Extraction Kit ( By Trizol)25ml
    OP-3023-50mlPro NuEx-Total RNA Extraction Kit ( By Trizol)50ml


    Components25ml50ml
    Total RNA Extraction (Trizol)25ml50ml
    ProtocolOneOne

    Additional Information:

  • Item Code: OP-3023
  • Delivery Time: 5-7 Business days
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    Chennai - 600091, Tamil Nadu, India
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