BIO TECH KITS

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Most of the protocol consists of 2 steps, first lysing the cell and solubilizing the DNA, which is followed by enzymatic or chemical method to remove contaminating proteins, RNA and other macromolecules. The rigid cell wall of the plant cell is weakened by EDTA in the Solution I. Then the cells are subjected to osmotic shock at 65°C to lyse the rigid cell wall of the plant cells. The cellular proteins are denatured by SDS in Solution II. Extraction with phenol denatures the protein, Chloroform removes SDS- Protein/ polysaccharide complexes as white interface between the aqueous and organic phase after centrifugation and isoamyl alcohol prevents foaming. The high molecular weight genomic DNA in aqueous phase is precipitated with 100% ethanol and further the salts are removed by washing with 70% ethanol.

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Approx. Rs 11,700 / KitGet Latest Price

The protocol is based on concentrating and separating intact chloroplast from tissue homogenate by using a combination of buffers and centrifugation. The cell debris, nuclei is first separated out by centrifugation at 1000 rpm and subsequently chloroplasts are pelleted at 4000 rpm. The nuclear DNA is digested with DNase and Mg/EDTA-Na₂. Subsequently the chloroplasts are lysed with 20% SDS to release the DNA. Deproteinization is carried out with phenol/chloroform and DNA extracted in ethanol, dried and dissolved in TE buffer.

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Approx. Rs 6,000 / PieceGet Latest Price

This kit is designed for fast isolation of genomic DNA from cell, bacteria. The kit contains a membrane embedded in a spin column for binding up to 10ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting Finger printing etc.

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Approx. Rs 6,600 / KitGet Latest Price

A phage lysate is used to prepare a large quantity of highly purified phage DNA. Host bacteria grown to A650~0.6 are infected with 105-108 phage /ml. Following infection, the cells are shaken vigorously until cell lysis occurs. Remaining labile cells are lysed with chloroform and all debris is removed with a low speed spin. The phage is concentrated by PEG precipitation (solution II). The purified phage capsids are then degraded and the DNA is isolated. 

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Cell lysis solutions are detergent-based, buffers and reagent sets that have been optimized for particular cell lysis applications. Effective cell lysis and protein extraction for different species of organisms and different cell and tissue types require different buffer formulations.

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Approx. Rs 4,850 / KitGet Latest Price

The plaque assay is originally a virological assay employed to count and measure the infectivity level of the bacteriophages. This assay is the most widely used technique for the isolation of virus and its purification, and to optimize the viral titers. The basis of plaque assay is to measure the ability of a single infectious virus to form a “plaque” on a concurrent monolayer culture cells. A plaque is developed as a part of infection of one cell by a single virus particle that is followed by the replication of that virus, and finally, the death of the cell. The newly replicated virus particles will later infect and then kill surrounding cells. The procedure requires the use of a Double-Layer Agar (DLA) technique also known as double agar overlay method, in which the hard agar serves 3 as a base layer (to form gel), and a mixture of few phage particles (diluted stock) and a very large number of host cells in a soft agar forms the upper overlay. When the plates are incubated, susceptible Escherichia coli cells multiply rapidly and produce a lawn of confluent growth on the medium. When one phage particle adsorbs to a susceptible cell, penetrates the cell, replicates and release new phage particles which infect other bacteria in the vicinity of the initial host cell. The growth or spread of the new viruses is then restricted or limited to the neighbouring cells by the gel. This cycle is repeated until large numbers of bacteria have been destroyed. The destroyed cells produce single circular, non turbid areas called plaques in the bacterial lawn, where there is no growth of bacteria because the phage progeny originating from single virus particles have multiplied sufficiently to kill bacteria over an easily visible area. Eventually the plaque becomes too large to be visible to our naked eye. Each plaque represents the lysis of a phage-infected bacterial culture and can be designated as a plaqueforming unit (PFU) and is used to quantitate the number of infective phage particles in the culture. The number of phage particles contained in the original stock phage culture is determined by counting the number of plaques formed on the seeded agar plate and multiplying this by the dilution factor. For a valid phage count, the number of plaques per plate should not exceed 300 nor be less than 30. Plates showing greater than 300 PFUs are too numerous to count (TNTC); plates showing fewer than 30 PFUs are too few to count (TFTC). The main difference between Plaque Assay and Colony-Counting is that, to count bacteria, we spread about 100 - 400 bacteria over the surface of the agar and incubate the plate. To count phage, we spread 100 - 400 phage particles mixed with a very large number of host bacterial cells over the surface of the agar and incubate the plate. In colony-counting, we directly observe the accumulation of large numbers of bacteria, each pile being a colony. In plaque formation, we directly observe the absence of bacteria against a background of dense bacterial growth, each zone of destruction being a phage plaque (hole) in the lawn of bacteria. Each hole represents one phage in the sample applied to the plate.

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Many different methods and technologies are available for the isolation of genomic DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

The choice of a method depends on many factors: the required quantity and molecular weight of the DNA, the purity required for downstream applications, and the time and expense. Home-made methods often work well for researchers who have developed and regularly use them. However, they usually lack standardization and therefore yields and quality are not always reproducible. Reproducibility is also affected when the method is used by different researchers, or with different sample types.

The separation of DNA from cellular components can be divided into four stages:

1.     Disruption

2.     Lysis

3.     Removal of proteins and contaminants

4.     Recovery of DNA

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                     This kit is designed for fast isolation of genomic DNA from plant source. The kit contains a membrane embedded in a spin column for binding up to 10ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting Finger printing etc.

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Approx. Rs 5,600 / KitGet Latest Price

• Soil Extract Agar (RT)
• Nutrient broth (RT)
• Agar ) (RT)
• Crystal violet (RT)
• Grams iodine (RT)
• Ethyl alcohol (RT)
• Safranin (RT)
• Glass slide (RT)
• Protocol

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• 50X TAE (RT)
• Agarose (RT)
• Ethidium Bromide (RT)
• Gel Loading Dye (RT
• Primer(F R) (-20 DegreeC)
• Taq Polymerase (-20 DegreeC)
• Template DNA (-20 DegreeC)
• 10 mM dNTP mix (-20 DegreeC)
• 10X Taq polymerase Buffer (-20 DegreeC)
• Distilled water (RT)
• Marker DNA (-20 DegreeC)
• PCR Tubes (RT)
• Protocol

20 Exp

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The kit contains a membrane embedded in a spin column for binding up to 1-5ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR and other molecular technique.

Components:

50X TAE (RT)

Agarose (RT)

column with 2ml collection

tube (RT)

Elution buffer (4°C)

Ethidium bromide (RT)

Gel loading dye (RT)

Lysis buffer (4°C)

TE Buffer (RT)

Wash buffer (RT)

Protocol

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 This is a general reaction given by deoxy-pentoses. The 2-deoxyribose of DNA, in the presence of acid, is converted to ω-hydroxilevulinic aldehyde, which reacts with diphenylamine to form a blue coloured complex, which can be read at 595 nm.

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Many different methods and technologies are available for the isolation of chromosomal DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

Components:

E.coli strain (4°C)

50X TAE (RT)

LB media (RT)

Phenol/Chloroform (25:24) (RT)

Proteinase K (4°C)

SDS (RT)

TE Buffer (RT)

3M Sodium acetate pH 5.2 (RT)

Agarose (RT)

DNA Marker (-20°C)

Ethanol (70%) (RT)

Ethidium Bromide (RT)

Gel loading dye (RT)

Isopropanol (100%) (RT)

Protocol

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Approx. Rs 6,000 / KitGet Latest Price

This kit is designed for fast isolation of genomic DNA from plant source. The kit contains a membrane embedded in a spin column for binding up to 10ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting Finger printing etc.

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Approx. Rs 3,500 / KitGet Latest Price

The amount of potassium is determined by using sodium tetraphenylboron in a specifically prepared mixture to produce a colloidal suspension. The turbidity of which is proportional to potassium concentration in the range of 2 - 7 mEq/L.

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