BIO TECH TEACHING KIT - Molecular biology

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First lysing the cell and solubilizing the DNA, which is followed by enzymatic or chemical method to remove contaminating proteins, RNA and other macromolecules. The rigid cell wall of the plant cell is weakened before proceeding with further steps.

Components:

3M Sodium acetate (RT)

70%Ethanol (RT)

Chloroform/ isoamyl alcohol (24:1) (RT)

Extraction buffer (RT)

Isopropanol (RT)

TE Buffer (RT)

Beta mercapto ethanol (RT)

50X TAE (RT)

Agarose (RT)

Ethidium Bromide (RT)

Gel loading dye (RT)

RNase A (-20°C)

Protocol

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Approx. Rs 9,300 / PieceGet Latest Price

• Agarose (RT)
• 50XTAE (RT)
• Gel loading dye (RT)
• Ethidium Bromide (RT)
• EcoRI (-20 DegreeC)
• Restriction buffer (-20 DegreeC)
• T4 Ligase (-20 DegreeC)
• Ligation buffer (-20 DegreeC)
• Templet DNA (-20 DegreeC)
• Taq polymerase (-20 DegreeC)
• Taq buffer (-20 DegreeC)
• dNTP 10mM (-20 DegreeC)
• MgCl2 (RT)
• EcoRI Primer (-20 DegreeC)
• EcoRI adaptors (F-R) (-20 DegreeC)
• PstI (-20 DegreeC)
• PstI adaptors (-20 DegreeC)
• Protocol

5 Exp

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• Alkaline lysis solution I (RT)
• Alkaline lysis solution II (RT)
• Alkaline lysis solution III (RT)
• Control Plasmid DNA (-20 DegreeC)
• Isopropanol (RT)
• E.coli Culture (-20 DegreeC)
• TE (pH 8.0)
• RNase A (-20 DegreeC)
• 50X TAE (RT)
• 70% Ethanol (RT)
• Agarose (RT)
• Ethidium Bromide (RT)
• Gel loading buffer (RT)
• Protocol

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Approx. Rs 8,500 / PieceGet Latest Price

The factors (inducer) which influence the expression of foreign proteins in Escherichia coli under the transcriptional control of the lac and tac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of an inducer IPTG (isopropyl--D-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.

Components:

ONPG (4°C)

Buffer A (RT)

E.coli (4°C)

Inducer (20mg/ml) (-20°C)

Lysis Buffer (RT)

1.5ml Microfuge tube (RT)

LB broth (RT)

Stop Solution (RT)

Mercaptoethanol (RT)

Protocol

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Approx. Rs 4,330 / PieceGet Latest Price

A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

Components:

50X TAE (RT)

Agarose (RT)

100% Ethanol (RT)

Ethidium Bromide (RT)

Gel loading Dye (RT)

TNES Buffer (RT)

70% Ethanol (RT)

Tris EDTA (RT)

DNA Marker (-20°C)

Protocol

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Approx. Rs 3,550 / PieceGet Latest Price

When DNA is treated with diphenylamine under the acidic condition a bluish green colored complex is formed which has an absorption peak at 595nm.This reaction is given by 2 -deoxypentose in general. In acidic solution deoxypentose are converted into a highly reactive. hydroxyl leavulinic aldehyde which reacts with diphenylamine gives bluish green colored complex. The colour intensity was measured using a red filter

Components:

Standard DNA (-20°C)

Test DNA (-20°C)

Diphenylamine solution (RT)

Protocol

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Approx. Rs 4,600 / PieceGet Latest Price

Many different methods and technologies are available for the isolation of chromosomal DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

Components:

E.coli strain (4°C)

50X TAE (RT)

LB media (RT)

Phenol/Chloroform (25:24) (RT)

Proteinase K (4°C)

SDS (RT)

TE Buffer (RT)

3M Sodium acetate pH 5.2 (RT)

Agarose (RT)

DNA Marker (-20°C)

Ethanol (70%) (RT)

Ethidium Bromide (RT)

Gel loading dye (RT)

Isopropanol (100%) (RT)

Protocol

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Approx. Rs 7,000 / PieceGet Latest Price

• Control DNA I (-20°C)
• Control DNA II (-20°C)
• Test DNA (-20°C)
• 10mM dNTP (-20°C)
• MgCl2 (-20°C)
• Taq Buffer
• Taq polymerase (-20°C)
• Random primer (-20°C)
• 1Kb DNA ladder (-20°C)
• Agarose (RT)
• Ethidium bromide (RT)
• Gel loading Dye (RT)
• 50X TAE (RT)
• Protocol

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Approx. Rs 4,500 / PieceGet Latest Price

• 50X TAE Buffer (RT)
• Agarose (RT)
• Distilled Water (RT)
• Ethidium Bromide (RT)
• Gel loading dye (RT)
• DNA (-20 DegreeC)
• ECoR I (-20 DegreeC)
• Hind III (-20 DegreeC)
• Restriction Buffer (-20 DegreeC)
• Protocol

20 Exp

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• Agarose (RT)
• Ethidium Bromide (RT)
• Formaldehyde(RT)
• Gel Loading dye (RT)
• MOPS 10 X (RT)
• RNA sample (-20 DegreeC)
• Transfer buffer (RT)
• Transfer membrane (RT)
• Blocker (4 DegreeC)
• Conjugate (4 DegreeC)
• Prehybridization Buffer (4 DegreeC)
• Probe (biotinylated) (-20 DegreeC)
• Substrate (4 DegreeC)
• Wash Buffer (RT)
• Protocol

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Approx. Rs 4,200 / PieceGet Latest Price

Bacterial plasmids are known to harbor genes for: (i) resistances to antibiotics and metals, (ii) catabolic pathways such as lactose utilization and degradation of hydrocarbons (iii) biosynthesis of certain antibiotics, etc. In many cases the characteristics of the host organism conferred by the plasmids remain elusive, and such cryptic plasmids are abundant in nature. Curing of this cryptic plasmid from a bacterial strain is a method to substantiate the relationship between a genetic trait and carriage of that specific trait in the plasmid. Various methods involving chemical and physical agents have been developed to eliminate plasmids. Protocols for curing plasmids consist frequently of exposure of a culture to sub-inhibitory concentrations of some chemical agents, e.g. acridine orange, acriflavine, and sodium dodecyl sulfate or to a super-optimal temperature followed by selection of cured derivatives.

Components:

E.coli host (4°C)

50X TAE (RT)

6X Gel loading dye (RT)

Agar (RT)

Agarose (RT)

LB broth (RT)

Ampicillin (4°C)

Chloroform (4°C)

Ethidium Bromide (RT)

Microfuge Tubes (RT)

Phenol (4)

Protocol

Additional Information: