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OPRL Biosciences Pvt.,Ltd
OPRL Biosciences Pvt.,Ltd


BIO TECH Teaching Kits

Microbiology:

Microbiology is the study of Microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells).
Microbiology techniques includes Isolationof Microbes ,Staining & Growth kinetics and tissue culture techniques.

Simple Staining Teaching Kit

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Simple Staining Teaching Kit
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BrandOrange

The purpose of simple staining is to elucidate the morphology and arrangements of bacterial cells in milk. The commonly used basic stains are Methelene Blue, Crystal Violet, and Carbol Fuchsin.

CAT.NOPRODUCTEXPS
OP-4111-25xpPro TechEx-Simple Staining Teaching Kit25Exps

Components
  • Methylene blue (RT)
  • Glass slides (RT)
  • Bacterial Culture (4 DegreeC)
  • Absorbent papers (RT)
  • Protocol

 

MOQ 25 Exps

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Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator) Teaching Kit

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Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator) Teaching Kit
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Chicken eggs were used for preparation of cell culture at 11,12, or 13 days of incubation. The disinfected egg’s air-sac region were cut and the embryo was removed from the shell. The embryo’s fibroblast region was processed with trypsinization.


Cat. NOProductExperiments
OP-4302-5xpPro TechEx-Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator) Teaching Kit5 Exps

MTT (4°C)

Cell line (4°C)

DMEM (4°C)

Trypsin (4°C)

FBSA (4°C)

Tryphan blue (RT)

PBS (RT)

Calf serum(4°C)


Additional Information:

  • Item Code: OP-4302
  • Delivery Time: 5-7 Business days
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    Culture Of HeLa S3 Cells In Suspension ( without CO2 System) Teaching Kit

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    Culture Of HeLa S3 Cells In Suspension ( without CO2 System) Teaching Kit
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    Hela cells can be grown to the appropriate density usually 70% in a humidified chamber at 37°C. HeLa cells in many labs are cultured in 75 cm2 flasks (or medium flasks). HeLa cells are grown in Dulbecco's Modified Eagle Medium with10% fetal bovine serum (FCS) and 1% MEM non-essential amino acids with penicillin-streptomycin at 1% is added to the culture media.


    Cat. NOProductExperiments
    OP-4301-5xpPro TechEx- Culture Of HeLa S3 Cells In Suspension ( without CO2 System) Teaching Kit5 Exps

    Components:

    · FBS(Fetal Bovine Serum)(4°C)

    · Cell line (4°C)

    · CO2 IGM (4°C)

    · Tryphan blue (RT)

    · Protocol


    Additional Information:

  • Item Code: OP-4301
  • Delivery Time: 5-7 Business days
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    MTT Assay Teaching Kit

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    MTT Assay Teaching Kit
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    The MTT assay is a colorimetric assay for assessing cell viability. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple colort. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of potential medicinal agents and toxic materials. MTT assays are usually done in the dark since the MTT reagent is sensitive to light.

    Cat.No Product Experiments 
    OP-4401-5xpPro TechEx-MTT Assay Teaching Kit5 Exps

    Components:

    MTT (4°C)DMSO (RT)96-Well Plate(RT)Cell culture(4°C)PBS(RT)Protocol

    Additional Information:

  • Item Code: OP-4401-5xp
  • Delivery Time: 5-7 Business days
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    Plant Tissue Culture Teaching Kit

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    Plant Tissue Culture Teaching Kit
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    Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrientn culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.

    Cat.No Product Experiments 
    OP-4201-5xpPro TechEx-Plant Tissue Culture Teaching Kit5 Exps


    Components
    Callus induction media (4°C)
    Hormones 1 (4°C)
    Shoot induction media (4°C)
    Hormones 2 (4°C)
    Root induction media (4°C)
    Hormones 3 (4°C)
    10% Bleach sterilizing solution (4°C)
    70% alcohol (4°C)

    Additional Information:

  • Item Code: OP-4201-5xp
  • Delivery Time: 5-7 Business days
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    Bacterial Growth Curve Teaching Kit

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    Bacterial Growth Curve Teaching Kit
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    Bacterial growth is the asexual reproduction, or cell division, of a bacterium into two daughter cells, in a process called binary fission. Providingno mutational event occurs the resulting daughter cells are genetically identical to the original cell. Hence, "local doubling" of the bacterialpopulation occurs. Both daughter cells from the division do not necessarily survive. However, if the number surviving exceeds unity on average, the bacterial population undergoes exponential growth. The measurement of an exponential bacterial growth curve in batch culture was traditionally a part of the training of all microbiologists; the basic means requires bacterial enumeration (cell counting) by direct and individual can estimate by calorimetric method.

    Cat.No Product Experiments 
    OP-4002-5xpPro TechEx-Bacterial Growth Curve Teaching Kit5 Exps
    OP-4002-10xpPro TechEx-Bacterial Growth Curve Teaching Kit10 Exps


    Components:

    Agar (RT)LB broth (RT)Bacterial culture (4°C)Protocol

    Additional Information:

  • Item Code: OP-4002
  • Delivery Time: 5-7 Business days
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    Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit

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    Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit
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    The effect to which salt concentration causes changes in bacterial growth depends on the osmotic balance required for such growth. Some bacteria require an astonishingly high level of salt to begin growth, whereas other bacteria would be immediately killed in high levels of salt.

    Cat.No Product Experiments 
    OP-4004-5xpPro TechEx-Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit5 Exps


    Components:

    Bacterial Culture (4°C)LB Broth (RT)Agar(RT)NaCl(RT)Protocol

    Additional Information:

  • Item Code: OP-4004-5xp
  • Delivery Time: 5-7 Business days
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    Isolation And Identification Of Enzyme Producing Microorganism Teaching Kit

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    Isolation And Identification Of Enzyme Producing Microorganism Teaching Kit
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    After the incubation, the presence or absence of the starch in the inoculated medium determines the ability of microorganisms to excretehydrolytic extra cellular enzymes capable of degrading the starch. The detection based on the hydrolytic activity of the organism, starch in the presence of iodine will impart a blue-black colour to the medium, indicating the absence of starch split by the enzymes and represent a negative reaction. If the starch has been hydrolyzed a clear zone of hydrolysis will surround the growth of the organism, representing a positive reaction.

    Cat.No Product Experiments 
    OP-3902-5xpPro TechEx -Isolation And Identification Of Enzyme Producing Microorganism Teaching Kit5 Exps


    Components:

    Starch agar (RT)Gram's Iodine (RT)Protocol

    Additional Information:

  • Item Code: OP-3902-5xp
  • Delivery Time: 5-7 Business days
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    Isolation And Identification Of Yeast From Curd Or Fruit Juice Teaching Kit

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    Isolation And Identification Of Yeast From Curd Or Fruit Juice Teaching Kit
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    Yeasts are unicellular eukaryotes ranging from 8-15μm in width. They can be isolated from many sources such as curd and fruit juices etc., using suitable media like sabouraud dextrose agar, yeast malt agar etc. One of the industrially important strains of the yeast is Saccharomyces cerevisiae which is used in the production of alcohol, bakery items, food stuffs, animal feed and biochemicals.

    Cat.No Product Experiments 
    OP-3905-5xpPro TechEx-Isolation And Identification Of Yeast From Curd Or Fruit Juice Teaching Kit5 Exps


    Components:

    YPD (RT)Agar (RT)Streptomycin (4°C)Protocol

    Additional Information:

  • Item Code: OP-3905-5xp
  • Delivery Time: 5-7 Business days
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    Isolation Of Molds From Soil Teaching Kit

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    Isolation Of Molds From Soil Teaching Kit
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    Great variability and type organism present in soil, it is impossible to isolate all microorganisms by single method. Therefore it is necessaryto use different media for growth of organisms. To support the growth of fungi different media (for instance: Sabouraud Dextrose Agar- SDA) are employed to isolate different kind of moulds from the soil samples. The fungal colonies are obtained from the soil sample after 3-4 days of incubation. Genera of the isolated colonies can be identified by lactophenol cotton blue staining.

    Cat.No Product Experiments 
    OP-3906-5xpPro TechEx-Isolation Of Molds From Soil Teaching Kit5 Exps


    Components:

    Sabouraud Dextrose agar (RT)Normal Saline (RT)lactophenol cotton blue (RT)Protocol

    Additional Information:

  • Item Code: OP-3906-5xp
  • Delivery Time: 5-7 Business days
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    Effect of Different pH on Bacterial Growth Teaching Kit

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    Effect of Different pH on Bacterial Growth Teaching Kit
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    BrandOrange


    Five 50 ml samples of LB broth were buffered with acetic acid at pH 4 and 5, with MES at pH 6 and with HEPES at pH 7 and 8. Each sample of medium was transferred to a 250 ml flask and 0.5 ml of a saturated overnight ulture of E. coli was added to each flask. A sample was taken from each flask at times 0, 0.5, 1, 2, 3, and 4 hours. The amount of bacteria in each sample was measured using a spectrophotometer at 595nm. The OD(595nm) and time data were plotted to show growth curves at each pH tested.

    CAT.NOPRODUCTEXPS
    OP-4003-5xpPro TechEx-Effect Of different pH On Bacterial Growth Teaching Kit5Exps

    Components
    • Bacterial culture (4 DegreeC)
    • Alkali Buffer (RT)
    • LB Broth (RT)
    • Agar (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-4003-5xp
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    Determination of Apoptosis By Staining Teaching Kit

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    Determination of Apoptosis By Staining Teaching Kit
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    Product Details:
    Shelf Life1 Month
    Sample Volume5 Exps
    Result Time1 Day
    Accuracy0.99

    Description:

    Determination of apoptosis By Staining Dye is primary method. Two types of dyes are used to study apoptosis are Hematoxylin Staining and Giemsa Staining.

    CAT.NOPRODUCTEXPS
    OP-4402-5xpPro TechEx-Determination Of Apoptosis By Staining Teaching Kit5Exps

    Components
    • Cell line (4 DegreeC)
    • DMEM (4 DegreeC)
    • Hematoxylin (4 DegreeC)
    • Giemsa stain (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-4402
  • Delivery Time: 5-7 Business days
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    Isolation And Identification Of Rhizobium From Root Nodules Teaching Kit

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    Isolation And Identification Of Rhizobium From Root Nodules Teaching Kit
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    Product Details:
    Usage/ApplicationLaboratory

    Bacteria of genus Rhizobium are fast growing Gram-negative rods that are normally involved in nitrogen fixation for the atmosphere. Rhizobia are commonly found in the root nodules of legumes;Yeast mannitol agar (YMA) has been recommended for the isolation of Rhizobium, so we used this media for our protocol. YMA contains yeast extract, which provides the bacteria with a nitrogen source, a necessary element for natural Rhizobium function. YMA also contains mannitol, a major one of the many carbohydrates that Rhizobium can utilize for metabolism.On the majority of the plates, white, mucilaginous, raised, semi-translucent colonies confirms the result for Rhizobium. This fits the Bergey's manual description of genus Rhizobium as forming "copious extracellular polysaccharide slime."

    Cat. NoProductExperiments
    OP-3903-5xpPro TechEx- Isolation And Identification Of Rhizobium From Root Nodules Teaching Kit5 Exps


    Components
    YEMA Medium (3.2gm/100ml) (RT)
    70%Ethanol (RT)
    0.1% mercuric chloride (RT)
    Carbol fuchsin (RT)
    Protocol

    Additional Information:

  • Item Code: OP-3903-5xp
  • Delivery Time: 5-7 Business days
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    Antibiotic Sensitivity Test Teaching Kit

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    Antibiotic Sensitivity Test Teaching Kit
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    Product Details:
    Usage/ApplicationLaboratory

    Antibiotic sensitivity is the susceptibility of bacteria to antibiotics. Antibiotic susceptibility testing (AST) is usually carried out to determine which antibiotic will be most successful in treating a bacterial infection in vivo. Testing for antibiotic sensitivity is often done by the Kirby-Bauer method. Small wafers containing antibiotics are placed onto a plate upon which bacteria are growing. If the bacteria are sensitive to the antibiotic, a clear ring, or zone of inhibition, is seen around the wafer indicating poor growth.

    Cat. NoProductExperiments
    OP-4001-5xpPro TechEx-Antibiotic Sensitivity Test Teaching Kit5 Exps

    Components
    E. coli (4°C)
    S. aureus (4°C)
    B. subtilis (4°C)
    Tetracycline (4°C)
    Ampicillin (4°C)
    Kanamycin (4°C)
    Mueller-Hinton broth (RT)
    Agar (RT)
    Gel Punch (RT)

    Additional Information:

  • Item Code: OP-4001-5xp
  • Delivery Time: 5-7 Business days
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    Isolation And Identification Of Soil Bacteria Teaching Kit

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    Isolation And Identification Of Soil Bacteria Teaching Kit
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    Product Details:
    Usage/ApplicationLaboratory

    Isolation of bacteria was performed by making serial dilution of the taken samples and the dilution used for studies were 10-2 and 10-4 . The dilution was spread in Nutrient agar medium plates and media was weighed out and prepared according to the manufacture's specification, with respect to the given instructions and directions. The plates were incubated at 37 DegreeC for 48 hrs. The Pure cultures were obtained based on the MOQ 5 Exps

    color of colony and the pure cultures of the bacterial isolates were subjected to various morphological and biochemical characterization tests to determine the identity of the bacteria and identification done by gram staining method.
    CAT.NOPRODUCTEXPS
    OP-3904-5xpPro TechEx-Isolation And Identification Of Soil Bacteria Teaching Kit5Exps

    Components
    • Soil Extract Agar (RT)
    • Nutrient broth (RT)
    • Agar ) (RT)
    • Crystal violet (RT)
    • Grams iodine (RT)
    • Ethyl alcohol (RT)
    • Safranin (RT)
    • Glass slide (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3904
  • Delivery Time: 5-7 Business days
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    Isolation & Identification Of Antibiotic Producing Microorganism Teaching Kit

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    Product Details:
    Shelf Life1 Month
    Result Time1 Day
    Accuracy0.99

    A sample of organisms from soil or some other source is diluted in water, then spread onto Petri dishes containing agar gel rich in the nutrients the bacteria will need to grow. Scientists select plates that have a large number of colonies, then look for microorganisms that have inhibited the growth of other microorganisms in their vicinity. These microbes are possibly secreting some kind of compound that is killing or inhibiting their neighbors. The isolation of antibiotic and identification of microbes by this method is known as crowded plate technique.

    Cat. NoProductExperiments
    OP-3901-5xpPro TechEx -Isolation And Identification Of Antibiotic Producing Microorganism Teaching Kit5 Exps


    Components
    Culture (4°C)
    Toothpicks (RT)
    Nutrient agar (RT)
    Protocol

    Additional Information:

  • Item Code: OP-3901-5xp
  • Delivery Time: 5-7 Business days
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