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Transcriptomics
Providing you the best range of pro techex-total rna extraction from bacteria teaching kit, pro techex-total rna extraction and rt-pcr teaching kit, pro techex-estimation of rna by orcinol method teaching kit, pro techex-total rna isolation from yeast by trizol method teaching kit, pro techex-plant rna isolation teaching kit and pro techex-preparation of poly(a) mrna from yeast teaching kit with effective & timely delivery.Pro TechEx-Total RNA Extraction From Bacteria Teaching Kit
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Approx. Rs 4,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| Number of preps | 10 |
| Model Name/Number | OP-3308-10xp |
| Brand | PROGENE |
| Usage/Application | RNA Extraction Kit |
| Storage Temperature | RT & 4C |
| Type | Total RNA Extraction from Bacteria Teaching Kit |
| Country of Origin | Made in India |
Mid-exponential phase bacteria grown in liquid media (50-100µl pellet volume) were harvested by centrifugation at 13500 rpm for 20s in a Microfuge tube, washed in detergent solution. Repelleted and resuspended in RNase free distilled water. Re-extracted with water saturated phenol. Isolated RNA was visualized by Ethidium Bromide Stained Denaturing Formaldehyde gel electrophoresis.
| CAT.NO | PRODUCT | EXPS |
| OP-3308-5xp | Pro TechEx-Total RNA Extraction from Bacteria Teaching Kit | 5 Exps |
| OP-3308-20xp | Pro TechEx- Total RNA Extraction from Bacteria Teaching Kit | 20 Exps |
Components
- Microfuge tubes (RT)
- 10X MOPS (RT)
- 70% Ethanol (RT)
- Agarose (RT)
- E.coli Host (4 DegreeC)
- Ethidium bromide (RT)
- Gel loading dye (RT)
- Agarose (RT)
- DEPC water (RT)
- LB medium (RT)
- Solution I (4 DegreeC)
- Solution II (4 DegreeC)
- Solution III (4 DegreeC)
- Solution IV (4 DegreeC)
- Protocol
MOQ-20 Exps
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Pro TechEx-Total RNA Extraction and RT-PCR Teaching Kit
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Approx. Rs 13,200 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Number of preps | 10 |
| Model Name/Number | OP-3307-10xp |
| Brand | PROGENE |
| Usage/Application | RNA Extraction Kit |
| Country of Origin | Made in India |
| Type | PCR Application Teaching Kit |
Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. In RT-PCR, the RNA template is first converted into a complementary DNA (cDNA) using a reverse transcriptase. The cDNA is then used as a template for exponential amplification using PCR. RT-PCR is currently the most sensitive method of RNA detection available.RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.
| CAT.NO | PRODUCT | EXPS |
| OP-3307-10xp | Pro TechEx-Total RNA Extraction and RT-PCR Teaching Kit | 10 Exps |
Components
- 1.5ml Microfuge tubes (RT)
- 10mM dNTP (-20 DegreeC)
- 10X MOPS (RT)
- Agarose (RT)
- Ethidium Bromide (RT)
- Gel Loading dye (RT)
- Primer F (-20 DegreeC)
- Yeast Culture (4 DegreeC)
- 0.2ml PCR tubes (RT)
- Primer R (-20 DegreeC)
- RNase free Water (4 DegreeC)
- Solution I (4 DegreeC)
- Solution II (4 DegreeC)
- Solution III (4 DegreeC)
- Solution IV (4 DegreeC)
- Yeast broth (RT)
MOQ-10 Exp
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Pro TechEx-Estimation of RNA by Orcinol Method Teaching Kit
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Approx. Rs 3,500 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| Number of preps | 50 |
| Brand | Progene |
| Model Name/Number | OP-3301-5xp |
| Sample Type | RNA |
| Usage/Application | Laboratory |
| Storage Temperature | 4C & RT |
| Elution Volume | 5 |
| Type | Manual |
| Humidity Resistant | Yes |
| Country of Origin | Made in India |
This is a general reaction for pentoses and depends on the formation of furfural when the pentose is heated with concentrated hydrochloric acid. Orcinol reacts with the furfural in the presence of ferric chloride as a catalyst to give a green colour, which can be measured at 665 nm.
| CAT.NO | PRODUCT | EXPS |
| OP-3301-5xp | Pro TechEx-Estimation of RNA by Orcinol Method Teaching Kit | 5 Exps |
Components
- 20X Diluent buffer (RT)
- 6% Orcinol Reagent (RT)
- Ferric chloride, anhydrous (RT)
- Standard RNA(1mg/ml) (-20 DegreeC)
- Test RNA Solution (-20 DegreeC)
- Protocol
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Pro TechEx-Total RNA isolation from Yeast by Trizol method Teaching Kit
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Approx. Rs 5,450 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| Number of preps | 10 |
| Brand | Progene |
| Model Name/Number | OP-3309-5xp |
| Sample Type | Cells |
| Shelf Life | 12 Months |
| Usage/Application | Laboratory |
| Accuracy | 0.99 |
| Humidity Resistant | Yes |
This protocol uses Trizol (also known as TRI REAGENT) for the isolation of total RNA. Trizol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, RNA and protein on homogenization or lysis of tissue sample. After adding chloroform and centrifuging, the mixture separates into 3 phases with the upper clear aqueous phase containing the RNA. The next steps in the extraction are washes and precipitation.
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Pro TechEx-Plant RNA Isolation Teaching Kit
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Approx. Rs 4,650 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| Number of preps | 10 |
| Brand | Progene |
| Model Name/Number | OP-3305-5xp |
| Sample Type | Tissues |
| Usage/Application | RNA Extraction Kit |
| Storage Temperature | RT, 4C, -20C |
| Elution Volume | 20ul |
| Type | Plant RNA Isolation Teaching Kit |
| Country of Origin | Made in India |
Isolation of pure, under graded total RNA from plant tissue can be difficult because of high levels of ribonucleases, pectins and polyphenols that are present in plant material. Another major problem is the presence and removal of proteins. This problem is commonly solved by lysing the plant cells in the presence of phenol/chloroform.
| CAT.NO | PRODUCT | EXPS |
| OP-3305-5xp | Pro TechEx - Plant RNA Isolation Teaching Kit | 5 Exps |
| OP-3305-10xp | Pro TechEx- Plant RNA Isolation Teaching Kit | 10 Exps |
Components
- 10X MOPS buffer (RT)
- Agarose (RT)
- Chloroform Solution (4°C)
- Ethidium Bromide (RT)
- Extraction buffer (4°C)
- Gel loading Dye (RT)
- Phenol:Chloroform Solution (4°C)
- Sodium acetate pH 5.2 (RT)
- 70% ethanol (RT)
- Absolute ethanol (4°C)
- DEPC-treated water (RT)
- Formaldehyde (RT)
- Marker (RT)
- Protocol
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Pro TechEx-Preparation Of Poly(A) MRNA From Yeast Teaching Kit
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Approx. Rs 5,400 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Brand | Progene |
| Model Name/Number | OP-3306-5xp |
| Usage/Application | Laboratory |
| Packaging Size | Box |
To isolate mRNA from total RNA sample, cellulose resin with a poly deoxythimidine oligomer attached (oligo-dT cellulose) is used to purify transcript with a poly-A tail. This purification should be done on the same day as the total RNA purification limit degradation. The total RNA and oligo-dT cellulose are incubated at 65°C and eluted by using elution buffer. Purified mRNA can be used for Reverse transcription process.
| Cat. NO | Product | Experiments |
| OP-3306-5xp | Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit | 5 Exps |
| OP-3306-10xp | Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit | 10 Exps |
Components:
· 10X MOPS (-20°C)
· Binding buffer (RT)
· Elution buffer (RT)
· Ethanol (RT)
· Oligo dT Cellulose (-20°C)
· Sodium acetate (RT)
· Total RNA (-20°C)
· 70% ethanol (RT)
· Agarose (RT)
· DEPC water (RT)
· Ethidium Bromide (RT)
· Formaldehyde (RT)
· Gel loading Dye (RT)
· Protocol
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Pro TechEx-Northern Hybridization Teaching Kit
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Approx. Rs 10,700 / KitGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 1 Kit |
| ICMR Approved | No |
| Brand | Progene |
| Usage/Application | Laboratory |
| Grade Standard | Progene |
| Physical State | Liquid |
| Catalog Number | OP-3304-5xp |
| Result Time | 2 days |
| Shelf Life | 12 Months |
| Humidity Resistant | Yes |
Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members. Northern Blotting involves separation of RNA samples according to size by agarose gel electrophoresis and detection with hybridization probe complementary to part of or the entire target sequence. Northern Blot refers to capillary transfer of RNA from the electrophoresis gel to the blotting membranes.
| CAT.NO | PRODUCT | EXPS |
| OP-3304-5xp | Pro TechEx-Northern Hybridization Teaching Kit | 5 Exps |
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