Chromatography Apparatus

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Amino acids are biologically active substances, and a number of them are essential for living beings. Amino acids are found in living cells as well as in body fluids of higher animals, in amounts, which vary according to the tissue and particular amino acid. Ion exchange columns have been utilized in a number of different ways for the isolation of aminoacids. The use of Dowex50X(200-400mesh) has superior resolving power Strong, insoluble structure—DOWEX fine mesh resins are based on a microporous copolymer of styrene and divinylbenzene (DVB) that results in maximum resistance to oxidation, reduction, mechanical wear and breakage.

Dowex 50 separates a complex mixture of peptides into a series of simpler mixtures. After the chromatogram is completed, the success of the separation is assessed by an examination of the fractions of effluent. The color reaction with ninhydrin is most frequently applied to determine in which fractions the peptides have emerged.

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