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Bio Tech Teaching Kit- Proteomics

These Proteomics Teaching Kits are mainly consumed in dept of Biotechnology, where Proteomics is consider as a part of major subject.

Protease Assay Kit

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Protease Assay Kit
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BrandOrange

Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.

Cat. NOProductExperiments
OP-3228-10ExPro Tech Ex- Protease assay kit10 Exps
OP-3228-20ExPro Tech Ex- Protease assay kit20 Exps

Components
Reagent B (Casein)
Reagent C (TCA)
FolinCiocalteu’s phenol reagent
Reagent E (Buffer)
Reagent G (Tyrosine standard)
Reagent H (Enzyme solution)

Additional Information:

  • Item Code: OP-3228
  • Delivery Time: 5-7 Business days
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    Protein Isolation From E.Coli Kit

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    Protein Isolation From E.Coli Kit
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    This kit is designed to rapidly extract denatured proteins from bacteria. The kit contains sufficient materials for extraction of total proteins from E.coli culture.

    Cat. NOProduct Experiments
    OP-3229-10ExPro Tech Ex-Protein isolation from e.coli Kit10 Exps
    OP-3229-20Ex Pro Tech Ex-Protein isolation from e.coli Kit20 Exps

    Components 
    Lysis buffer
    protease inhibitor cocktail
    Dnase 1
    Ammonium sulphate
    Dialysis membrane
    10X PBS
    Separating gel mix
    Stacking gel mix
    TEMED
    APS
    SDS 10%
    Running buffer
    Stainer
    Destainer

    Additional Information:

  • Item Code: OP-3229
  • Delivery Time: 5-7 Business days
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    Agrobacterium Mediated Gene Transfer Teaching Kit

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    Agrobacterium Mediated Gene Transfer Teaching Kit
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    Plant Infection The most studied Agrobacterium disease is crown gall disease caused by A. tumefaciens. Gall formation of this disease is initiated at the flesh wound site of the host plant. From there the bacteria transfer its DNA into the host cell where it incorporates itself into the host genome.

    Cat. NOProductExperiments
    OP-3232-5XpAgrobacterium Mediated Gene Transfer Teaching Kit5 Exps
    OP-3232-10XpAgrobacterium Mediated Gene Transfer Teaching Kit10 Exps

    ComponentsSTORAGE
    Antibiotics4°C
    Agrobacterium slant4°C
    YEP MediumRT
    MS Medium4°C
    HgCl2+tween 20RT
    AgarRT
    ProtocolRT

    Additional Information:

  • Item Code: OP-3232
  • Delivery Time: 5-7 Business days
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    Study of Protein Fingerprinting Teaching Kit

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    Study of Protein Fingerprinting Teaching Kit
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    Study of protein composition of various organs of an organism shall provide insight into the extent of protein diversity. The electrophoretic separation of proteins on a SDS-PAGE results in Protein finger printing.The objective of this Kit is:Familiarize students with diversity of Proteins from different organs of the same organism.1. Make students understand the diversity of proteins leads to diversity of organ functioning.2. Identification of sample by Protein finger printing (SDS - PAGE).
    Cat. NOProductExperiments
    OP-3226-5xpPro TechEx-Study of Protein Fingerprinting Teaching Kit5 Exps
    OP-3226-15xpPro TechEx-Study of Protein Fingerprinting Teaching Kit15 Exps

    Components
    10%SDS(RT)
    Destainer(RT)
    Microfuge tubes(RT)
    Running gel buffer(RT)
    Separating buffer (RT)
    Stacking buffer(RT)
    Stainer(RT)
    40¬rylamide(4°C)
    APS (0.1g/ml)(4°C)
    Protein Extract-Heart (-20°C)
    Protein Extract-Kidney (-20°C)
    Protein Extract-Liver(-20°C)
    Protein Extract-Lung (-20°C)
    Sample solubilizing buffer (4°C)
    TEMED (4°C)
    Protocol

    Additional Information:

  • Item Code: OP-3226
  • Delivery Time: 5-7 Business days
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    Clone Gene Analysis by SDS - PAGE Teaching Kit

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    Clone Gene Analysis by SDS - PAGE Teaching Kit
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    Expression of a recombinant protein can be approached in general by constructing a plasmid that encodes the desired protein, introducing the plasmid into the required host cell, growing the host cells and inducing protein expression, and then lysing the cells, purifying the protein, and performing SDS-PAGE analysis to verify the presence of the protein .Protein expression can be induced by a rising of the incubation temperature or by the addition of an inducing chemical such as isopropyl-b-D-thiogalactoside (IPTG) to the culture medium.

    CAT.NOPRODUCTEXPS
    OP-3207-5xpPro TechEx-Clone Gene Analysis by SDS - PAGE Teaching Kit5 Exps
    OP-3207-10xpPro TechEx-Clone Gene Analysis by SDS - PAGE Teaching Kit10 Exps

    Components
    • Ampicillin (4 DegreeC)
    • Inducer (IPTG)(4 DegreeC)
    • LB broth powder(RT)
    • Non Recombinant bacterial culture (4 DegreeC)
    • Recombinant bacterial culture (4 DegreeC)
    • Separating gel mix(4 DegreeC)
    • TEMED(4 DegreeC)
    • APS (ammonium per sulphate)(4 DegreeC)
    • Destainer(RT)
    • Running gel buffer mix (powder) (RT)
    • Sample solubilizing buffer(4 DegreeC)
    • Stacking gel mix(4 DegreeC)
    • Stainer (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3207
  • Minimum Order Quantity: 5 Number
  • Delivery Time: 5-7 Business days
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    Protein Agarose Gel Electrophoresis Teaching Kit

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    Protein Agarose Gel Electrophoresis Teaching Kit
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    Protein electrophoresis in agarose gel is an alternative approach to using polyacrylamide gels and provides several benefits. Gels can be run using a vertical system or a horizontal system and unlike polyacrylamide gels,agarose gels can be used effectively to separate proteins larger than 600,000 Da.

    CAT.NOPRODUCTEXPS
    OP-3216-10xpPro TechEx-Protein Agarose Gel Electrophoresis Teaching Kit10 Exps

    Components

    • 2X Destainer (RT)
    • 5X Running Buffer (RT)
    • Agarose(RT)
    • Protein Sample(4 DegreeC)
    • Sample Buffer(4 DegreeC)
    • Stainer(RT)
    • Protocol

    MOQ 10 Exps


    Additional Information:

  • Item Code: OP-3216
  • Delivery Time: 5-7 Business days
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    Histone Isolation From Nuclei Teaching Kit

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    Histone Isolation From Nuclei Teaching Kit
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    1. A new method has been developed for the specific extraction of histone fraction f2 (a) from calf thymus deoxyribonucleoprotein at pH7 by using a mixture of ethanol and guanidinium chloride.2. Fraction f2 (a) has been separated into the sub fractions f2(a)1 and f2(a)2 by acetone precipitation from acid solution, and at pH7. 3. Modifications of existing electrophoretic methods are described that enable these fractions to be more easily characterized.
    CAT.NOPRODUCTEXPS
    OP-3210-5xpPro TechEx-Histone Isolation From Nuclei Teaching Kit5 Exps

    Components

    • Chromatin Sample (4 DegreeC)
    • Denaturation buffer (4 DegreeC)
    • Ethanol (RT)
    • Extraction buffer (4 DegreeC)
    • Solubilizing Buffer (RT)
    • SSC Buffer (4 DegreeC)
    • APS(4 DegreeC)
    • Running buffer (RT)
    • Sample solublizing buffer(4 DegreeC)
    • SDS 10% (RT)
    • Separating gel mix (4 DegreeC)
    • Stacking gel mix(4 DegreeC)
    • TEMED(4 DegreeC)
    • Destainer (RT)
    • Protein I(4 DegreeC)
    • Protein II(4 DegreeC)
    • Protein III(4 DegreeC)
    • Protein Marker(4 DegreeC)
    • Stainer(RT)
    • Protocol

    MOQ-5 Exps


    Additional Information:

  • Item Code: OP-3210
  • Delivery Time: 5-7 Business days
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    Analysis of Protein Structure Teaching Kit

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    Analysis of Protein Structure Teaching Kit
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    This kit is designed to understand the structure of proteins by following technique listed below:1. Study the role of disulphide bonds in maintaining protein structure.2. Role of heat denaturation on protein structure.3. Use of electrophoresis for characterization of proteins.4. Use of SDS-PAGE for analysis of Protein structure.

    Cat.No Product Experiments 
    OP-3201-5xpPro TechEx-Analysis of Protein Structure Teaching Kit5 Exps
    OP-3201-15xpPro TechEx-Analysis of Protein Structure Teaching Kit15 Exps


    Components
    10% SDS (RT)Acrylamide (4°C)Protein I (-20°C)
    Destainer (RT)APS (4°C)Protein II(-20°C)
    Running buffer (RT)Microfuge tubes(RT)Protein marker I(-20°C)
    Separating Buffer(RT)Reducing agent (4°C)Protein marker II(-20°C)
    Stacking buffer (RT)Sample solubilizing buffer (4°C)Protein marker III(-20°C)
    Stainer (RT)TEMED (4°C)Protocol

    Additional Information:

  • Item Code: OP-3201
  • Delivery Time: 5-7 Business days
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    Study of Cellular Fractional Teaching Kit

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    Study of Cellular Fractional Teaching Kit
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    This protocol has been adapted to fractionate a variety of different cell types into cytoplasm, nucleoplasm and nucleoli. It uses a hypotonic buffer to swell the cells prior to dounce homogenization to break them open.

    Cat.NoProductExperiments
    OP-3224-5xpPro TechEx-Study of cellular Fractionation Teaching Kit5 Exps


    Components:

    Buffer A (4°C)
    S1 (4°C)
    S2 (4°C)
    S3 (4°C)
    RIPA buffer (4°C)
    Protocol

    Additional Information:

  • Item Code: OP-3224-5xp
  • Delivery Time: 5-7 Business days
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    Antibody FITC Conjugation Teaching Kit

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    Antibody FITC Conjugation Teaching Kit
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    FITC is most commonly-used fluorescent dye for FACS analysis. FITC is a small organic molecule, and is typically conjugated to proteins via primary amines (i.e., lysines). Usually, between 3 and 6 FITC molecules are conjugated to each antibody; higher conjugations can result in solubility problems as well as internal quenching (and reduced brightness). Thus, an antibody will usually be conjugated in several parallel reactions to different amounts of FITC, and the resulting reagents will be compared for brightness (and background stickiness) to choose the optimal conjugation ratio. Fluorescein is typically excited by the 488 nm, and emission is collected at 530 nm.

    Cat.No Product Experiments 
    OP-3203-5xpPro TechEx-Antibody FITC Conjugation Teaching Kit5 Exps


    Components:

    Antibody (-20°C)Antigen Spotted Strip(4°C)FITC(-20°C)Mounting buffer (4°C)Glass Slides (RT)Substrate(4°C)10X PBS (RT)BCIP/NBT (4°C)Dialysis Bag (RT)Dialysis Clip (RT)Washing Buffer (RT)Protocol

    Additional Information:

  • Item Code: OP-3203-5xp
  • Delivery Time: 5-7 Business days
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    Antibody HRP Conjugation Teaching Kit

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    Antibody HRP Conjugate Kit contains a horseradish peroxidase-conjugated Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognizes histidine residues regardless of the surrounding amino acid content. The purified antibody conjugate produces a strong signal at a 1:1000-1:2000 dilution on Western blots and dot blots. Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, purification or sorting applications.

    Cat.No Product Experiments 
    OP-3204-5xpPro TechEx-Antibody HRP Conjugation Teaching Kit5 Exps


    Components:

    10X PBS (RT)Antibody(-20°C)Blocker(RT)Horse radishperoxidase(HRP)(-20°C)Hydrogen peroxide (H2O2)(RT)Pre-coated strips(4°C)Substrate(4°C)96-Well Plate(RT)Dialysis bag(RT)Dialysis clip(RT)Glutaraldehyde(RT)Stopping solution(RT)Washing buffer(RT)Protocol

    Additional Information:

  • Item Code: OP-3204
  • Delivery Time: 5-7 Business days
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    IgY Purification and Analysis by SDS -PAGE Teaching Kit

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    IgY Purification and Analysis by SDS -PAGE Teaching Kit
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    The method involves two important steps. The first one is the removal of lipids and the second is the precipitation of total IgY from the supernatant of step one. After dialysis against a buffer (normally PBS) the IgY-extract can be stored at -20°C for more than a year. The purity of the extract is around 80%, the total IgY per egg varies from 40-80 mg.

    Cat.No Product Experiments 
    OP-3211-5xpPro TechEx-IgY Purification and analysis by SDS -PAGE Teaching Kit5 Exps
    OP-3211-10xpPro TechEx-IgY Purification and analysis by SDS -PAGE Teaching Kit10 Exps


    Components
    Precipitation solution I (RT)APS(4°C)Destainer (RT)
    Precipitation solution II (RT)Running buffer (RT)Protein I(4°C)
    Phosphate buffer saline (RT)SDS 10% (RT)Protein II(4°C)
    Syringe (RT)Separating gel mix (4°C)Protein III(4°C)
    Sample solublising buffer(4°C)Stacking gel mix(4°C)Protein Marker(4°C)
    ProtocolTEMED(4°C)Stainer(RT)

    Additional Information:

  • Item Code: OP-3211
  • Delivery Time: 5-7 Business days
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    Immunohistochemistry Teaching Kit

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    Immunohistochemistry Teaching Kit
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    Immunohistochemistry is the localization of antigens or proteins in tissue sections by the use of labeled antibodies as specific reagentsthrough antigen-antibody interactions that are visualized by a marker such as enzyme.

    Cat.No Product Experiments 
    OP-3212-5xpPro TechEx-Immunohistochemistry Teaching Kit5 Exps


    Components:

    Primary antibody (-20°C)Secondary antibody HRP conjugate (-20°C)Blocking Solution (-20°C)DAB Reagent (-20°C)Ethanol 100% (RT)Hematoxylin (RT)Solution I (RT)Solution II (RT)
    Hydrogen Peroxide (RT)Paraffin tissue slide (+ve) (RT)Paraffin tissue slide (–ve) (RT)Wash buffer (RT)DPX mountant (RT)10X PBS (RT)Coverslips(RT)Protocol

    Additional Information:

  • Item Code: OP-3212-5xp
  • Delivery Time: 5-7 Business days
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    Isoelectrofocusing and 2-D Gel Electrophoresis (Tube Gel ) Teaching Kit

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    Isoelectrofocusing and 2-D Gel Electrophoresis (Tube Gel ) Teaching Kit
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    Isoelectric focusing methods are widely applied for the separation of proteins, peptides and enzymes. In a pH gradient the sample components migrate towards the anode or the cathode to the pH values, where their net charges are zero is called isoelectric point (pI).

    Cat.No Product Experiments 
    OP-3213-10xpPro TechEx-Isoelectrofocusing and 2-D Gel Electrophoresis (Tube Gel ) Teaching Kit10 Exps


    Components
    4X Separating gel buffer (4°C)Ampholytes (4°C)Destainer (RT)
    4X Stacking gel buffer (4°C)APS (4°C)Glycine (RT)
    Acrylamide monomer (4°C)IEF Sample Buffer (4°C)Parafilm (RT)
    Equilibrium Buffer (4°C)NaOH (RT)SDS (RT)
    Gel Monomer Solution (4°C)Phosphoric acid (RT)Stainer (RT)
    Protein Sample (4°C)TEMED (4°C)Syringe (RT)
    Sample Overlay Solution(4°C)Urea (RT)Tris (RT)
    Protocol20% Nonidet P-40 (NP–40) (4°C)

    Additional Information:

  • Item Code: OP-3213-10xp
  • Delivery Time: 5-7 Business days
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    Preparation of Biotinylated Probes by Nick Translation Teaching Kit

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    Preparation of Biotinylated Probes by Nick Translation Teaching Kit
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    This method utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporate labeled nucleotides as the polymerase examines, or “proofreads” the nicked site. Large plasmids, cosmids, and PCR fragments are all regularly used with this method, and the DNA can be linearized or supercoiled. Individual templates produce consistent results in the standard 90-minutes reaction, and result in an average probe length of 200 bp up to 500 bp.

    Cat.No Product Experiments 
    OP-3215-5xpPro TechEx-Preparation of Biotinylated Probes by Nick Translation Teaching Kit5 Exps


    Components
    Biotin-16-dUTP (-20°C)2M Magnesium chloride(RT)1M Tris-HCl (RT)
    Bovine serum albumin (BSA) (-20°C)b-Mercaptoethanol, 99%(RT)Sterile Water (RT)
    Dig-11-dUTP (-20°C)Glycerol (RT)Protocol
    DNase I from bovine pancreas (-20°C)Lambda HindIII marker (-20°C)
    dNTP Mix (-20°C)Polymerase (-20°C)
    0.5 M EDTA (RT)1 M NaCl (RT)

    Additional Information:

  • Item Code: OP-3215-5xp
  • Delivery Time: 5-7 Business days
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    NATIVE - PAGE Teaching Kit

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    NATIVE - PAGE Teaching Kit
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    Native PAGE uses the same discontinuous chloride and glycine ion fronts as SDS-PAGE to form moving boundaries that stack and then separate polypeptides by charge to mass ratio. Proteins are prepared in a non-reducing non-denaturing sample buffer, which maintains the protein’s secondary structure and native charge density. Therefore you can easily see multiple bands from the camshot of your native PAGE gel if your target protein has polymerized forms in your sample. In native PAGE electrophoresis most proteins have an acidic or slightly basic pl(isoelectric point) (~3–8) and migrate towards the negative polar.

    Cat.No Product Experiments 
    OP-3214-10xpPro TechEx-NATIVE - PAGE Teaching Kit10 Exps
    OP-3214-20xpPro TechEx-NATIVE - PAGE Teaching Kit20 Exps


    Components:

    APS(4°C)Running buffer (RT)Sample solublising buffer(4°C)Separating gel mix (4°C)Stacking gel mix(4°C)TEMED(4°C)Destainer (RT)Protein I(4°C)Protein II(4°C)Protein III(4°C)Protein Marker(4°C)Stainer(RT)Protocol

    Additional Information:

  • Item Code: OP-3214
  • Delivery Time: 5-7 Business days
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    Western Blotting Teaching Kit

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    Western Blotting Teaching Kit
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    Western blotting is an analytical method wherein a protein sample is electrophoresed on an SDS-PAGE and electrotransferred onto nitrocellulose membrane. The transferred protein is detected using specific primary antibody and secondary enzyme labeled antibody and substrate. A protein sample is subjected to polyacrylamide gel electrophoresis. After this the gel is placed over a sheet of nitrocellulose and the protein in the gel is electrophoretically transferred to the nitrocellulose. The nitrocellulose is then incubated with the specific antibody for the protein of interest. The nitrocellulose is then incubated with a second antibody, which is specific for the first antibody.

    Cat.No Product Experiments 
    OP-3227-5xpPro TechEx-Western Blotting Teaching Kit5 Exps
    OP-3227-20xpPro TechEx-Western Blotting Teaching Kit20 Exps

    Components
    APS (4°C)10X PBST (RT)10% SDS (RT)
    Running buffer (RT)DAB (-20°C)Blocker (4°C)
    SDS Sample buffer (4°C)Destainer(RT)H2O2(4°C)
    Separating gel mix (4°C)Nitrocellulose membrane (RT)Methanol (RT)
    Stacking gel mix (4°C)Primary antibody (-20°C)Protein sample(-20°C)
    TEMED (4°C)Secondary antibody HRP-conjugate (-20°C)Substrate buffer (4°C)
    Transfer buffer (RT)Stainer (RT)Protocol

    Additional Information:

  • Item Code: Op-3227
  • Delivery Time: 5-7 Business days
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    Silver Staining Teaching Kit

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    Silver Staining Teaching Kit
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    Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) . The rationale of silver staining is quite simple. Proteins bind silver ions, which can be reduced under appropriate conditions to build up a visible image made of finely divided silver metal.

    Basic steps involved in silver stainingi) Fixation to get rid of interfering compounds.ii) Sensitization and rinses to increase the sensitivity and contrast of the staining.iii) Silver impregnation with either a silver nitrate solution or a silver-ammonia complex solution.iv) Rinses and development to build up the silver metal image v) stop and rinse to end development prior to excessivebackground formation and to remove excess silver ion and other chemicals prior to further processing.

    Cat.No Product Experiments 
    OP-3222-10xpPro TechEx-Silver Staining Teaching Kit10 Exps


    Components:

    Cross linking Solution 5X (RT)Developing Solution 5X (RT)Fixing Solution 5X (RT)
    Formaldehyde (RT)Protein Sample (4°C)Reducing Agent 5X (RT)
    Sample Solublizing Buffer (4°C)Silver Nitrate Solution 5X (RT)Protocol

    Additional Information:

  • Item Code: OP-3222-10xp
  • Delivery Time: 5-7 Business days
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    Serum Protein Electrophoresis Teaching Kit

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    Serum Protein Electrophoresis Teaching Kit
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    The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. Proteins are substances made up of smaller building blocks called amino acids. Proteins carry a positive or a negative electrical charge, and they move in fluid when placed in an electrical field. Serum protein electrophoresis uses an electrical field to separate the proteins in the blood serum into groups of similar size, shape, and charge.       Blood serum contains two major protein groups: albumin and globulin. Both albumin and globulin carry substances through the bloodstream. Using protein electrophoresis, these two groups can be separated into five smaller groups (fractions).

    Cat.No Product Experiments 
    OP-3221-10xpPro TechEx- Serum Protein Electrophoresis Teaching Kit10 Exps


    Components:

    Agarose (RT)Electrophoresis buffer (RT)Sample buffer (4°C)Protein sample (4°C)Ponceau’s stain (RT)Protocol

    Additional Information:

  • Item Code: OP-3221-10xp
  • Delivery Time: 5-7 Business days
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    SDS- PAGE Teaching Kit

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    SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used in biochemistry, genetics and molecular biology to separate proteins according to their molecular weight. The electrophoretic mobility of proteins depends upon their size. As proteins are amphoteric compounds, their net charge can therefore be determined by the pH of the medium in which they are suspended. Therefore, at a given pH and under non-denaturing conditions, the electrophoretic separation of proteins is determined by both size and charge of molecules. As proteins are high molecular weight molecules, it needs porous gels to get separated. Polyacrylamide gels are those which provide a means of separating proteins by size as they are porous.

    Cat.No Product Experiments 
    OP-3220-10xpPro TechEx-SDS- PAGE Teaching Kit10 Exps
    OP-3220-20xpPro TechEx-SDS- PAGE Teaching Kit20 Exps


    Components:

    APS(4°C)Running buffer (RT)Sample solublising buffer(4°C)SDS 10% (RT)Separating gel mix (4°C)Stacking gel mix(4°C)TEMED(4°C)Destainer (RT)Protein I(4°C)Protein II(4°C)Protein III(4°C)Protein Marker(4°C)Stainer(RT)Protocol

    Additional Information:

  • Item Code: OP-3220-10xp
  • Delivery Time: 5-7 Business days
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    Protein Folding Teaching Kit

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    Protein folding is the process by which a protein structure assumes its functional shape or conformation. It is the physical process by which a polypeptide folds into its characteristic and functional three-dimensional structure from random coil.

    Cat.No Product Experiments 
    OP-3219-5xpPro TechEx-Protein Folding Teaching Kit5 Exps
    OP-3219-15xpPro TechEx-Protein Folding Teaching Kit15 Exps


    Components:

    Separating buffer (RT)Stacking buffer(RT)Running Gel Buffer (RT)SDS (RT)Acrylamide (4°C)Sample solubilizing buffer (RT)ProtocolAPS (RT)70% Isopropanol (RT)TEMED (4°C)Protein Lysate (4°C)Protein Marker (4°C)Microfuge tubes (RT)

    Additional Information:

  • Item Code: OP-3219
  • Delivery Time: 5-7 Business days
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    Immunoglobulin G Isolation from Serum

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    Cat.No Product Experiments 
    OP-3231-5XpPro Tech Ex-Immunoglobulin G isolation from serum5 Exps


    Components:

    Serum sample Control IgG(0.25mg/ml)Caprylic acid Ammonium sulphate Dialysis Membrane10X PBS

    Additional Information:

  • Item Code: OP-3231-5xp-S
  • Delivery Time: 5-7 Business days
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    Bacterial Alkaline Phosphatase Activity Teaching Kit

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    Bacterial Alkaline Phosphatase Activity Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. Kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP. The Kit can detect 10-250 µU ALP in samples. Bacterial Alkaline Phosphatase (BAP) is a phosphomonoesterase that hydrolyzes 3′ and 5′ phosphates from DNA and RNA. It is suitable for removing 5′ phosphates prior to end-labeling and for dephosphorylating vectors prior to insert ligation. BAP is active at 65 DegreeC for at least 1 h. It is sensitive to inhibition by micromolar amounts of inorganic phosphate. The enzyme is purified from E. coli antibody HRP conjugate .Horseradish Peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by dialysis.

    CAT.NOPRODUCTEXPS
    OP-3205-5xpPro TechEx-Bacterial Alkaline Phosphatase Activity Teaching Kit5 Exps
    OP-3205-10xpPro TechEx-Bacterial Alkaline Phosphatase Activity Teaching Kit10 Exps

    Components
    • 1.5ml Microfuge Tubes (RT)
    • LB Broth (RT)
    • Lysis Buffer (RT)
    • Reaction Buffer (RT)
    • Stopping Solution (RT)
    • E.coli host (4 DegreeC)
    • Inducer (-20 DegreeC)
    • Substrate (-20 DegreeC)
    • Protocol

    MOQ-10 Exps


    Additional Information:

  • Item Code: OP-3205
  • Delivery Time: 5-7 Business days
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    Study of Calf Intestine Alkaline Phosphatase Activity Teaching Kit

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    BrandOrange
    Usage/ApplicationLaboratory


    Calf intestinal alkaline phosphatase (CIAP) is a phosphomonoesterase purified from calf intestinal mucosa that hydrolyzes 5'-phosphate groups from DNA, RNA, and nucleotides. CIAP is used to dephosphorylate linearized vector DNA prior to insert ligation and to remove 5'-phosphate groups prior to 5'-end labeling of nucleic acids with T4 polynucleotide kinase.

    CAT.NOPRODUCTEXPS
    OP-3223-5xpPro TechEx-Study of Calf Intestine Alkaline Phosphatase Activity
    ( Dephosporylation of DNA) Teaching Kit
    5 Exps

    Components
    • 3M Sodium Acetate (RT)
    • Calf Intestine Alkaline Phosphatase Solution(-20 DegreeC)
    • ChloroformIsoamyl Alcohol(241) (4 DegreeC)
    • DNA(-20 DegreeC)
    • Enzyme buffer (-20 DegreeC)
    • Restriction Enzyme (-20 DegreeC)
    • TE Buffer(RT)
    • Tris saturated phenol (4 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3223
  • Delivery Time: 5-7 Business days
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    Dialysis Kit

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    Dialysis Kit
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    Product Details:
    UsageHospital
    BrandOrange

    Cat. NOProductExperiments
    OP-3231-5 XpPro Tech Ex-Dialysis kit5 Exps

    Components
    Dialysis membrane
    10XPBS
    Dialysis clip
    Ammonium sulphate

    Additional Information:

  • Item Code: OP-3231-5Xp
  • Delivery Time: 5-7 Business days
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    Amino Acid Kit

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    Amino Acid Kit
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    Product Details:
    ModelOP-3230-1K
    BrandOrange

    Assay All 24 Amino acids in the kit are >99% in purityHomogeneity by TLC All 24 Amino acids in the kit are chrmatographically homogeneous by TLC.

    Cat. NOProductExperiments
    OP-3230-1KTech Ex-Amino acid kit1 Kit


    NOTES
    Amino acids contained are,
    DL-Alanine, DL-2-Aminobutyric acid,
    L-Arginine monohydrochloride,
    DL-Aspartic acid, L-Cysteine monohydrochloride,
    L-Cystine, DL-DOPA, L-Glutamic acid,
    Glycine, L-Histidine monohydrochloride,
    L-Hydroxyproline, DL-Isoleucine, DL-Norleucine,
    L-Leucine, L-Lysine monohydrochloride, DL-Methionine,
    L-Ornithine monohydrochloride, DL-Phenylalanine,
    L Proline, DL-Serine, DL-Threonine, L-Tryptophan,
    L-Tyrosine, DLValine

    Additional Information:

  • Item Code: OP-3230-1K
  • Delivery Time: 5-7 Business days
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    Protein Degradation Teaching Kit

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    This experiment is designed to teach the students how the protein is degraded by protease enzyme.This kit can be used to study,1. How Protein fragments are degraded,2. Study the action of protease in degradation of proteins into peptides.3. Protein electrophoresis is used to demonstrate the effect of protease on protein.

    CAT.NOPRODUCTEXPS
    OP-3217-5xpPro TechEx-Protein Degradation Teaching Kit5 Exps

    Components
    • Acrylamide (4 DegreeC)
    • APS (4 DegreeC)
    • Running Gel Buffer (RT)
    • SDS (RT)
    • Separating buffer (RT)
    • Stacking buffer(RT)
    • TEMED (4 DegreeC)
    • 70% Isopropanol(RT)
    • Microfuge tubes (RT)
    • Protein Sample(-20 DegreeC)
    • Proteinase K(-20 DegreeC)
    • Sterile water (RT)
    • Stop Solution (RT)
    • Protocol

    MOQ-5 Exp

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    Study of Physical Properties Of Protein Teaching Kit

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    Product Details:
    BrandOrange
    Usage/ApplicationResearch and Institute


    The experiment in this kit demonstrates the effect of an acid, salt, organic solubility of provided protein samples and how an anionic detergent has the effect of these parameters on protein precipitation.The results shall encourage students to seek answers as to how proteins in aqueous solution react with acid, alkali, salt, detergent and heat. How these factors affect protein structure in solubility.The results should help students to understand how altered physical properties can be exploited for protein Solubilization, purification and characterization studies.

    CAT.NOPRODUCTEXPS
    OP-3225-5xpPro TechEx-Study of Physical Properties of Protein Teaching Kit5 Exps
    OP-3225-15xpPro TechEx-Study of Physical Properties of Protein Teaching Kit15 Exps


    Components

    • Acid precipitant (RT)
    • Protein solution A (4 DegreeC)
    • Protein solution B (4 DegreeC)
    • Protein solution C (4 DegreeC)
    • Tissue extraction buffer (-20 DegreeC)
    • 1.5ml Microfuge tubes (RT)
    • Detergent solution (RT)
    • Organic Precipitant (RT)
    • Salt solution (RT)
    • Protocol

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  • Item Code: OP-3225-15xp
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    Karyotyping Teaching Kit

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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Plant Infection The most studied Agrobacterium disease is crown gall disease caused by A. tumefaciens. Gall formation of this disease is initiated at the flesh wound site of the host plant. From there the bacteria transfer its DNA into the host cell where it incorporates itself into the host genome.

    Cat. NoProductExperiments
    OP-3233-5XpKaryotyping Teaching Kit5 Exps
    OP-3233-10XpKaryotyping Teaching Kit10 Exps

    ComponentsSTORAGE
    RPMI4°C
    Colchicine Solution4°C
    Potassium chloride solutionRT
    Staining Solution4°C
    Tissue Culture FlaskRT
    Glass slides Fine polishedRT
    Centrifuge tubes, 15mlRT

    Additional Information:

  • Item Code: OP-3233
  • Delivery Time: 5-7 Business days
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    Enzyme Kinetics Teaching Kit

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    Usage/ApplicationLaboratory


    Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated.
    CAT.NOPRODUCTEXPS
    OP-3209-5xpPro TechEx-Enzyme Kinetics Teaching Kit5 Exps
    Components
    • Reaction buffer (RT)
    • Reaction buffer pH 6.0 (RT)
    • Reaction buffer pH 7.0 (RT)
    • Reaction buffer pH 8.0 (RT)
    • Reaction buffer pH 9.0 (RT)
    • Reaction buffer pH 10.0 (RT)
    • Substrate (4 DegreeC)
    • Substrate * (4 DegreeC)
    • Inhibitor(NaOH) (RT)
    • Enzyme (-20 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3209
  • Delivery Time: 5-7 Business days
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    Cellulose Acetate Electrophoresis Teaching Kit

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    Product Details:
    UsageLaboratory
    ModelOP-3206

    Cellulose acetate is an acetate salt of cellulose produced by treating cotton with acetic acid using sulphuric acid as a catalyst. Migration takes place on the buffer film on the surface of the cellulose acetate plate or membrane. Separation of the proteins is primarily by charge. Cellulose acetate electrophoresis may be used for qualitative identification of variants, but also with elution for quantitation of the haemoglobins, A2, A, S, D, Lepore, -chain variants, Hb H and Hb Bart's.

    Cat. NOProductExperiments
    OP-3206-10xpPro TechEx-Cellulose Acetate Electrophoresis Teaching Kit10 Exps

    Components
    Stainer(RT)
    Destainer(RT)
    Protein sample(RT)
    Running buffer(RT)
    Cellulose acetate strip(RT)
    Protocol

    Additional Information:

  • Item Code: OP-3206
  • Delivery Time: 5-7 Business days
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    Protein Estimation Teaching Kit

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    Product Details:
    Model NumberOP-3218
    Usage/ApplicationLaboratory

    Description:

    Protein Estimation Teaching Kit (Quantitative) enables the determination of the concentration of unknown proteins based on various quantitative tests. The Kit is designed for rapid and accurate determination of proteins by three most commonly used techniques, each having features that suit it to a particular use. It enables the determination of the concentration of proteins which is frequently required in biochemical work. Most protein assays take advantage of a reaction between a reagent dye and the protein of interest that will shift or increase the absorbance of a particular wavelength. The choice of the method depends on the nature of the protein, the nature of the other components in the protein sample, desired speed, accuracy and sensitivity of the assay.

    CAT.NOPRODUCTEXPS
    OP-3218-5xpPro TechEx-Protein Estimation Teaching Kit3 X 5 Exps

    Components

    • Protein sample (BSA) (4 DegreeC)
    • Unknown sample(4 DegreeC)
    • Reagent A (RT)
    • Reagent B (RT)
    • Reagent C (RT)
    • Bradford reagent (4 DegreeC)
    • Biuret reagent (RT)
    • Folin reagent (4 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3218
  • Delivery Time: 5-7 Business days
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    Antibody Biotin Conjugation Teaching Kit

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    Product Details:
    Usage/ApplicationLaboratory

    Description:

    Labeling antibodies by covalent coupling of a biotinyl group is simple and normally does not have any adverse effect on the antibody. Most biotinylations are performed using ester of biotin. The reagent reacts with primary amines of the lysine residues or the amino terminus on the antibody to form amide bonds. A water soluble analog of N-hydroxylsuccinimide biotin also used in these procedures.

    CAT.NOPRODUCTEXPS
    OP-3202-5xpPro TechEx-Antibody Biotin Conjugation Teaching Kit5 Exps

    Components
    • Antibody (-20 DegreeC)
    • Antigen Spotted Strip(4 DegreeC)
    • Biotin (-20 DegreeC)
    • Coupling buffer I (4 DegreeC)
    • Coupling buffer II (4 DegreeC)
    • Streptavidin ALP(-20 DegreeC)
    • 10X PBS (RT)
    • BCIP/NBT (4 DegreeC)
    • Dialysis Bag (RT)
    • Dialysis Clip (RT)
    • Washing Buffer (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3202
  • Delivery Time: 5-7 Business days
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    Enzyme Isolation from Bacteria Teaching Kit

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    Enzyme Isolation from Bacteria Teaching Kit
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    Product Details:
    Usage/ApplicationLaboratory

    Description:

    Lactate dehydrogenase (L-lactateNAD oxidoreductase (LDH) is a tetrameric protein catalyzing the reversible conversion of pyruvate to lactate. The enzyme uses NAD /NADH as cofactor and exists in six isoforms, five depending on the combination of the two subunits A (muscle) and B (heart), each with a molecular mass of 36 kDa and an additional homotetrameric LDH-C (testis)-isoform.

    CAT.NOPRODUCTEXPS
    OP-3208-5xpPro Tech Ex- Enzyme Isolation from Bacteria Teaching Kit5 Exps

    Components
    • 20XPBS (RT)
    • Ammonium Sulphate (RT)
    • APS (RT)
    • Extraction buffer (4 DegreeC)
    • SDS 10% (RT)
    • Separating gel mix (4 DegreeC)
    • Stacking gel mix (4 DegreeC)
    • TEMED (RT)
    • Running buffer (RT)
    • Control Protein (4 DegreeC)
    • Destainer (RT)
    • Dialysis membrane with Rubber (RT)
    • Sample solublising buffer (RT)
    • Stainer (RT)
    • Protocol

    5 Exp


    Additional Information:

  • Item Code: OP-3208
  • Delivery Time: 5-7 Business days
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