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OPRL Biosciences Pvt.,Ltd
OPRL Biosciences Pvt.,Ltd


BIO TECH Teaching kit - Chromatography Techniques

Chromatography is a Technique which seperates bio molecules (In BioTechnology)

Immobilized Metal Ion Affinity Chromatography Teaching Kit

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Immobilized Metal Ion Affinity Chromatography Teaching Kit
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IMAC is based on the interaction between molecules in solution and metal ions immobilized on a solid support. The molecules are separated according to their affinity for the chelated metal ions, which depends on the coordination between the chelated metal ion and electron donorgroups from the ligand. The ligands are assumed to bind to the metal through ligand exchange. Two characteristics of the metal–ligand bond can be used for the successful separation of different ligands.

Cat.No Product Experiments 
OP-3505-5xp Pro TechEx-Immobilized Metal Ion Affinity Chromatography Teaching Kit 5 Exps

Components
Crude enzyme sample (-20°C)
DEAE cellulose (-20°C)
Elution buffer (4°C)
Equilibration buffer (4°C)
Substrate (-20°C)
Wash buffer (RT)
Column (RT)
CuSo4 solution (RT)
Microfuge tubes (RT)
NaOH solution (RT)
Reaction buffer (RT)
Protocol

Additional Information:

  • Item Code: OP-3505
  • Delivery Time: 5-7 Business days
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    Ion Exchange Chromatography (Lysozyme Purification) Teaching Kit

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    Ion Exchange Chromatography (Lysozyme Purification) Teaching Kit
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    Ion exchange is probably the most frequently used chromatographic technique for the separation and purification of proteins, polypeptides, nucleic acids, polynucleotides, and other charged biomolecules . The reasons for the success of ion exchange are its widespread applicability, its high resolving power, its high capacity, and the simplicity and controllability of the method.


    Cat. NOProductExperiments
    OP-3508-5xpPro TechEx-Ion Exchange Chromatography (Lysozyme Purification) Teaching Kit5 Exps

    Components
    CM-Cellulose (4°C)
    Column (RT)
    Solution I (4°C)
    Solution II (4°C)
    1M NaCl (RT)
    Micrococcus luteus (4°C)
    Standard lysozyme(4°C)
    Phosphate Buffer (RT)
    Protocol

    Additional Information:

  • Item Code: OP-3508-5xp
  • Delivery Time: 5-7 Business days
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    Affinity Chromatography Teaching Kit

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    Affinity Chromatography Teaching Kit
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    Usage/ApplicationLaboratory

    Description:
    Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand.

    CAT.NOPRODUCTEXPS
    OP-3501-5xpPro TechEx-Affinity Chromatography Teaching Kit5Exps

    Components
    • Anti Rabbit IgG (antibody) (-20 DegreeC)
    • Elution buffer (4 DegreeC)
    • Equilibration buffer (4 DegreeC)
    • Protein A sepharose (4 DegreeC)
    • Serum Sample (4 DegreeC)
    • Washing buffer (RT)
    • Agarose (RT)
    • Chromatography column (RT)
    • Microfuge tubes (RT)
    • Normal saline (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3501-5xp
  • Delivery Time: 5-7 Business days
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    IgG Purification Teaching Kit

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    IgG Purification  Teaching Kit
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    Affinity chromatography is a particularly powerful procedure, which can be used to purify Immunoglobulin, sub populations of IgG, or the antigen binding fraction of IgG present in serum/ascitic fluid/hybridoma culture supernatent. This technique requires the production of the solid matrix to which a ligand having either affinity for the revelent IgG or Vice Versa has been bound.
    CAT.NOPRODUCTEXPS
    OP-3504-5xpPro TechEx-IgG Purification Teaching Kit5Exps

    Components
    • Anti Rabbit IgG (antibody) (4 DegreeC)
    • Elution buffer (4 DegreeC)
    • Equilibration buffer (4 DegreeC)
    • Protein A sepharose (4 DegreeC)
    • Serum Sample (4 DegreeC)
    • Washing buffer (RT)
    • Agarose (RT)
    • Chromatography column (RT)
    • Normal saline (RT)
    • Microfuge tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3504-5xp
  • Delivery Time: 5-7 Business days
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    Column Chromatography Teaching Kit

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    Column Chromatography Teaching Kit
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    Usage/ApplicationLaboratory

    Column chromatography is one of the most useful methods for the separation and purification of both solids and liquids. This is a solid - liquid technique in which the stationary phase is a solid & mobile phase is a liquid. The principle of column chromatography is based on differential adsorption of substance by the adsorbent.

    CAT.NOPRODUCTEXPS
    OP-3502-10xpPro TechEx-Column Chromatography Teaching Kit10Exps

    Components
    • Alumina (RT)
    • Benzene (RT)
    • Column (RT)
    • Methanol (RT)
    • Sodium sulphate (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3502-10xp
  • Delivery Time: 5-7 Business days
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    Ion Exchange Chromatography (Antibody Purification)

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    Ion Exchange Chromatography (Antibody Purification)
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    Ion Exchange chromatography is a powerful procedure, which can be used to purify IgG(antibody), sub populations of IgG, or the antigen binding fraction of IgG present in serum/ascitic fluid/hybridoma culture supernatent. This technique requires the production of the solid matrix (DEAE Cellulose)to which a ligand having either affinity for the revelent IgG or Vice Versa has been bound.
    CAT.NOPRODUCTEXPS
    OP-3507-5xpPro TechEx-Ion Exchange Chromatography (Antibody Purification ) Teaching Kit5Exps
    Components
    • 10X Equlibration buffer (4 DegreeC)
    • 10X wash buffer (4 DegreeC)
    • 5X Elution buffer (4 DegreeC)
    • DEAE cellulose (4 DegreeC)
    • Anti bovine IgG (4 DegreeC)
    • Crude sample (4 DegreeC)
    • Agarose (RT)
    • Normal saline (RT)
    • Column (RT)
    • Protocol

    MOQ-5 Exp


    Additional Information:

  • Item Code: OP-3507-5xp
  • Delivery Time: 5-7 Business days
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    Gel Filtration Chromatography Teaching Kit

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    Gel Filtration Chromatography Teaching Kit
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    Usage/ApplicationLaboratory

    Descriptions:
    Gel filtration chromatography (sometimes referred to as molecular sieve chromatography) is a method that separates molecules according to their size and shape. The separation of the components in the sample mixture, with some exceptions, correlates with their molecular weights. In these cases, gel filtration can be used as an analytical method to determine the molecular weight of an uncharacterized molecule. Gel filtration is also an important preparative technique since it is often a chromatographic step in the purification of proteins, polysaccharides and nucleic acids.
    CAT.NOPRODUCTEXPS
    OP-3503-5xpPro TechEx-Gel Filtration Chromatography Teaching Kit5Exps

    Components

    • 10X Equilibration Buffer (4 DegreeC)
    • Column (4 DegreeC)
    • Protein Mixture (-20 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3503-5xp
  • Delivery Time: 5-7 Business days
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    Immunoglobulin Purification Teaching Kit

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    Immunoglobulin Purification Teaching Kit
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    Product Details:
    Shelf Life1 MOnth
    Result Time1 Day
    Accuracy0.99

    Description:

    Affinity chromatography is a particularly powerful procedure, which can be used to purify Immunoglobulin, sub populations of Ig, or the antigen binding fraction of Ig present in serum/ascitic fluid/hybridoma culture supernatent. This technique requires the production of the solid matrix to which a ligand having either affinity for the revelent Immunoglobulin has been bound.
    CAT.NOPRODUCTEXPS
    OP-3506-5xpPro TechEx-Immunoglobulin Purification Teaching Kit5Exps

    Components
    • Anti Rabbit IgG (antibody) (4 DegreeC)
    • Elution buffer (4 DegreeC)
    • Equilibration buffer (4 DegreeC)
    • Protein A sepharose (4 DegreeC)
    • Serum Sample (4 DegreeC)
    • Washing buffer (RT)
    • Agarose (RT)
    • Chromatography column (RT)
    • Normal saline (RT)
    • Microfuge tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3506
  • Delivery Time: 5-7 Business days
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    Paper Chromatography Teaching Kit

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    Paper Chromatography Teaching Kit
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    Product Details:
    BrandOrange
    Usage/ApplicationLaboratory

    Paper Chromatography is a separation technique that is used to separate and identify the components of a mixture . Paper chromatography is used to identify colouring agents.

    Cat.NoProductExperiments
    OP-3509-10xpPro TechEx-Paper Chromatography Teaching Kit10 Exps

    Components
    Chromatography Paper (RT)
    Proline (4°C)
    Histidine (4°C)
    Iso- Leucine (4°C)
    Solvents (BAW) (RT)
    Ninhydrin Reagent (RT)
    Rf value standard chart sheet (RT)
    Protocol

    Additional Information:

  • Item Code: OP-3509-10xp
  • Delivery Time: 5-7 Business days
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    Thin Layer Chromatography Teaching Kit

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    Thin Layer Chromatography Teaching Kit
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    Product Details:
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    Usage/ApplicationLaboratory

    Thin-layer chromatography or TLC, is a solid-liquid form of chromatography where the stationary phase is normally a polar absorbent and the mobile phase can be a single solvent or combination of solvents. TLC is a quick, inexpensive microscale technique that can be used to:• Determine the number of components in a mixture• Verify a substance’s identity

    Cat.NoProductExperiments
    OP-3510-10xpPro TechEx-Thin Layer Chromatography Teaching Kit10 Exps

    Components
    TLC Plate (RT)
    Proline (4°C)
    Histidine (4°C)
    Leucine (4°C)
    Solvents (BAW) (RT)
    Ninhydrin Reagent (RT)
    Rf value standard chart sheet (RT)
    Protocol

    Additional Information:

  • Item Code: OP-3510-10xp
  • Delivery Time: 5-7 Business days
  • Yes! I am interested

     
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