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BIO TECH Teaching kit - Chromatography Techniques
Chromatography is a Technique which seperates bio molecules (In BioTechnology)Affinity Chromatography Teaching Kit
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Approx. Rs 5,100 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Brand | PROGENE |
| Country of Origin | Made in India |
| I Deal In | New Only |
| Type | Affinity Chromatography Teaching Kit |
| Portable | Yes |
Description:
Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand.
| CAT.NO | PRODUCT | EXPS |
| OP-3501-5xp | Pro TechEx-Affinity Chromatography Teaching Kit | 5Exps |
Components
- Anti Rabbit IgG (antibody) (-20 DegreeC)
- Elution buffer (4 DegreeC)
- Equilibration buffer (4 DegreeC)
- Protein A sepharose (4 DegreeC)
- Serum Sample (4 DegreeC)
- Washing buffer (RT)
- Agarose (RT)
- Chromatography column (RT)
- Microfuge tubes (RT)
- Normal saline (RT)
- Protocol
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Immunoglobulin Purification Teaching Kit
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Approx. Rs 6,200 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | PROGENE |
| Usage/Application | Laboratory |
| Shelf Life | 1 MOnth |
| Form | Liquid |
| Method | Elisa |
| Result Time | 1 Day |
| Accuracy | 0.99 |
| Material | Plastic |
| I Deal In | New Only |
Description:
Affinity chromatography is a particularly powerful procedure, which can be used to purify Immunoglobulin, sub populations of Ig, or the antigen binding fraction of Ig present in serum/ascitic fluid/hybridoma culture supernatent. This technique requires the production of the solid matrix to which a ligand having either affinity for the revelent Immunoglobulin has been bound.
| CAT.NO | PRODUCT | EXPS |
| OP-3506-5xp | Pro TechEx-Immunoglobulin Purification Teaching Kit | 5Exps |
Components
- Anti Rabbit IgG (antibody) (4 DegreeC)
- Elution buffer (4 DegreeC)
- Equilibration buffer (4 DegreeC)
- Protein A sepharose (4 DegreeC)
- Serum Sample (4 DegreeC)
- Washing buffer (RT)
- Agarose (RT)
- Chromatography column (RT)
- Normal saline (RT)
- Microfuge tubes (RT)
- Protocol
Additional Information:
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Immobilized Metal Ion Affinity Chromatography Teaching Kit
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Approx. Rs 14,900 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| I Deal In | New Only |
IMAC is based on the interaction between molecules in solution and metal ions immobilized on a solid support. The molecules are separated according to their affinity for the chelated metal ions, which depends on the coordination between the chelated metal ion and electron donorgroups from the ligand. The ligands are assumed to bind to the metal through ligand exchange. Two characteristics of the metal–ligand bond can be used for the successful separation of different ligands.
| Cat.No | Product | Experiments |
| OP-3505-5xp | Pro TechEx-Immobilized Metal Ion Affinity Chromatography Teaching Kit | 5 Exps |
| Components |
| Crude enzyme sample (-20°C) |
| DEAE cellulose (-20°C) |
| Elution buffer (4°C) |
| Equilibration buffer (4°C) |
| Substrate (-20°C) |
| Wash buffer (RT) |
| Column (RT) |
| CuSo4 solution (RT) |
| Microfuge tubes (RT) |
| NaOH solution (RT) |
| Reaction buffer (RT) |
| Protocol |
Additional Information:
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Ion Exchange Chromatography (Lysozyme Purification) Teaching Kit
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Approx. Rs 5,100 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3508-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Ion exchange is probably the most frequently used chromatographic technique for the separation and purification of proteins, polypeptides, nucleic acids, polynucleotides, and other charged biomolecules . The reasons for the success of ion exchange are its widespread applicability, its high resolving power, its high capacity, and the simplicity and controllability of the method.
| Cat. NO | Product | Experiments |
| OP-3508-5xp | Pro TechEx-Ion Exchange Chromatography (Lysozyme Purification) Teaching Kit | 5 Exps |
| Components |
| CM-Cellulose (4°C) |
| Column (RT) |
| Solution I (4°C) |
| Solution II (4°C) |
| 1M NaCl (RT) |
| Micrococcus luteus (4°C) |
| Standard lysozyme(4°C) |
| Phosphate Buffer (RT) |
| Protocol |
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IgG Purification Teaching Kit
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Approx. Rs 5,450 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Number of Reactions(Preps)/Kit | 5xp |
| Product Type | KIT |
| Packaging Size | 5xp |
| Catalog Number | OP-3504-5xp |
| Brand | progene |
| Country of Origin | Made in India |
Affinity chromatography is a particularly powerful procedure, which can be used to purify Immunoglobulin, sub populations of IgG, or the antigen binding fraction of IgG present in serum/ascitic fluid/hybridoma culture supernatent. This technique requires the production of the solid matrix to which a ligand having either affinity for the revelent IgG or Vice Versa has been bound.
| CAT.NO | PRODUCT | EXPS |
| OP-3504-5xp | Pro TechEx-IgG Purification Teaching Kit | 5Exps |
Components
- Anti Rabbit IgG (antibody) (4 DegreeC)
- Elution buffer (4 DegreeC)
- Equilibration buffer (4 DegreeC)
- Protein A sepharose (4 DegreeC)
- Serum Sample (4 DegreeC)
- Washing buffer (RT)
- Agarose (RT)
- Chromatography column (RT)
- Normal saline (RT)
- Microfuge tubes (RT)
- Protocol
Additional Information:
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Column Chromatography Teaching Kit
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Approx. Rs 4,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 10 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3502-10xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Column chromatography is one of the most useful methods for the separation and purification of both solids and liquids. This is a solid - liquid technique in which the stationary phase is a solid & mobile phase is a liquid. The principle of column chromatography is based on differential adsorption of substance by the adsorbent.
| CAT.NO | PRODUCT | EXPS |
| OP-3502-10xp | Pro TechEx-Column Chromatography Teaching Kit | 10Exps |
Components
- Alumina (RT)
- Benzene (RT)
- Column (RT)
- Methanol (RT)
- Sodium sulphate (RT)
- Protocol
Additional Information:
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Gel Filtration Chromatography Teaching Kit
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Approx. Rs 5,100 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Usage/Application | Laboratory |
| Brand | Progene |
| Material | Plastic |
| Shape | Rectangular |
| Color | Red |
| I Deal In | New Only |
Descriptions:
Gel filtration chromatography (sometimes referred to as molecular sieve chromatography) is a method that separates molecules according to their size and shape. The separation of the components in the sample mixture, with some exceptions, correlates with their molecular weights. In these cases, gel filtration can be used as an analytical method to determine the molecular weight of an uncharacterized molecule. Gel filtration is also an important preparative technique since it is often a chromatographic step in the purification of proteins, polysaccharides and nucleic acids.
| CAT.NO | PRODUCT | EXPS |
| OP-3503-5xp | Pro TechEx-Gel Filtration Chromatography Teaching Kit | 5Exps |
Components
- 10X Equilibration Buffer (4 DegreeC)
- Column (4 DegreeC)
- Protein Mixture (-20 DegreeC)
- Protocol
Additional Information:
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Thin Layer Chromatography Teaching Kit
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Approx. Rs 5,100 / PieceGet Latest Price
Product BrochureProduct Details:| Material | Plastic |
| Shape | Rectangular |
| Usage/Application | Laboratory |
| Color | Red |
| Brand | PROGENE |
| I Deal In | New Only |
Thin-layer chromatography or TLC, is a solid-liquid form of chromatography where the stationary phase is normally a polar absorbent and the mobile phase can be a single solvent or combination of solvents. TLC is a quick, inexpensive microscale technique that can be used to:• Determine the number of components in a mixture• Verify a substance’s identity
| Cat.No | Product | Experiments |
| OP-3510-10xp | Pro TechEx-Thin Layer Chromatography Teaching Kit | 10 Exps |
| Components |
| TLC Plate (RT) |
| Proline (4°C) |
| Histidine (4°C) |
| Leucine (4°C) |
| Solvents (BAW) (RT) |
| Ninhydrin Reagent (RT) |
| Rf value standard chart sheet (RT) |
| Protocol |
Additional Information:
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Paper Chromatography Teaching Kit
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Approx. Rs 4,750 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Brand | Progene |
| Color | Red |
| Material | Plastic |
| Shape | Rectangle |
| I Deal In | New Only |
Paper Chromatography is a separation technique that is used to separate and identify the components of a mixture . Paper chromatography is used to identify colouring agents.
| Cat.No | Product | Experiments |
| OP-3509-10xp | Pro TechEx-Paper Chromatography Teaching Kit | 10 Exps |
| Components |
| Chromatography Paper (RT) |
| Proline (4°C) |
| Histidine (4°C) |
| Iso- Leucine (4°C) |
| Solvents (BAW) (RT) |
| Ninhydrin Reagent (RT) |
| Rf value standard chart sheet (RT) |
| Protocol |
Additional Information:
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Ion Exchange Chromatography (Antibody Purification)
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Approx. Rs 5,300 / KitGet Latest Price
Product BrochureProduct Details:| Tests Kit | Antibody Purification |
| Type | Ion Exchange Chromatography |
| Brand | Progene |
| Country of Origin | Made in India |
| I Deal In | New Only |
Ion Exchange chromatography is a powerful procedure, which can be used to purify IgG(antibody), sub populations of IgG, or the antigen binding fraction of IgG present in serum/ascitic fluid/hybridoma culture supernatent. This technique requires the production of the solid matrix (DEAE Cellulose)to which a ligand having either affinity for the revelent IgG or Vice Versa has been bound.
| CAT.NO | PRODUCT | EXPS |
| OP-3507-5xp | Pro TechEx-Ion Exchange Chromatography (Antibody Purification ) Teaching Kit | 5Exps |
- 10X Equlibration buffer (4 DegreeC)
- 10X wash buffer (4 DegreeC)
- 5X Elution buffer (4 DegreeC)
- DEAE cellulose (4 DegreeC)
- Anti bovine IgG (4 DegreeC)
- Crude sample (4 DegreeC)
- Agarose (RT)
- Normal saline (RT)
- Column (RT)
- Protocol
MOQ-5 Exp
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