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Molecular Biology Teaching Kits

Molecular biology is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between the different types of DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated.

Bacterial Gene Expression Teaching Kit

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Bacterial Gene Expression Teaching Kit
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Bacterial Gene expression is the process by which information from a gene is used to the synthesis a functional gene product.

CAT.NOPRODUCTEXPS
OP-3104-5xpPro TechEx - Bacterial Gene Expression Teaching Kit5 Exps

Components
Recombinant bacterial culture (4 DegreeC)APS (RT)
Inducer(IPTG) (4 DegreeC)Sample Solubilizing buffer (4 DegreeC)
Ampicillin (4 DegreeC)Running gel buffer mix (RT)
LB broth powder (RT)Stainer (RT)
Separating gel mix (4 DegreeC)Destainer (RT)
Stacking gel mix (4 DegreeC)Protocol

Additional Information:

  • Item Code: OP-3104-5xp
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    Gel Extraction Teaching Kit

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    Gel Extraction Teaching Kit
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    MOQ20 exps

    In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps. This process, usually performed on plasmids.After DNA samples are run on an agarose gel, extraction involves four basic steps identifying the fragments of interest, isolating the corresponding bands, isolating the DNA from those bands, and removing the accompanying salts and stain.

    CAT.NOPRODUCTEXPS
    OP-3117-5xpPro TechEx-Gel Extraction Teaching Kit5 Exps
    OP-3117-20xpPro TechEx-Gel Extraction Teaching Kit20 Exps


    Components
    • 50X TAE (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • Silica powder (RT)
    • Solubilizing buffer (RT)
    • TE Buffer (RT)
    • Wash buffer (RT)
    • DNA sample (-20 Degree)
    • DNA marker (-20 Degree)
    • Agarose (RT)
    • Protocol


    Additional Information:

  • Item Code: OP-3117-20xp
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    DNA Extraction From Bacteria Teaching Kit

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    DNA Extraction From Bacteria Teaching Kit
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    Many different methods and technologies are available for the isolation of genomic DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

    CAT.NOPRODUCTEXPS
    OP-3121-10xpPro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit10 Exps
    OP-3121-20xpPro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit20 Exps
    Components

    • E.coli strain (4 DegreeC)
    • 50X TAE (RT)
    • LB media (RT)
    • Phenol/Chloroform (2524) (RT)
    • Proteinase K (4 DegreeC)
    • SDS (RT)
    • TE Buffer (RT)
    • 3M Sodium acetate pH 5.2 (RT)
    • Agarose (RT)
    • DNA Marker (-20 DegreeC)
    • Ethanol (70%) (RT)
    • Ethidium Bromide (RT)
    • Gel loading dye (RT)
    • Isopropanol (100%) (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3121-20xp
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    Competent Cell Preparation Teaching Kit

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    Competent Cell Preparation Teaching Kit
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    There are several ways to prepare competent cells for plasmid DNA transformation. This is the chemical method(CaCl2). Advantages are that it's simple to complete, requires no special equipment and gives good transformation efciencies.

    CAT.NOPRODUCTEXPS
    OP-3107-10xpPro TechEx -Competent Cell Preparation Teaching Kit10 Exps
    OP-3107-20xpPro TechEx -Competent Cell Preparation Teaching Kit20 Exps
    Components
    • E.coli Host (4 DegreeC)
    • Solution A (CaCl2) (4 DegreeC)
    • Agar (RT)
    • LB Broth (RT)
    • Glycerol (RT)
    • Protocol

    20 Exp

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    Denaturing Gel Electrophoresis

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    Denaturing Gel Electrophoresis
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    MOQ 10 Exps

    Denaturing Gel Electrophoresis for DNA Sequencing.

    Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single-stranded fragments of DNA or RNA that differ in length by as little as one nucleotide.
    Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all footprinting protocols. Thicker gels are often used to purify oligonucleotides.
    This appendix describes the pouring, running, and processing of a typical sequencing gel, which is 40 cm long with a uniform thickness of 0.4 mm,containing 7 M urea and 4% to 8% acrylamide.

    CAT.NOPRODUCTEXPS
    OP-3108-5xpPro TechEx - Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit5 Exps
    OP-3108-10xpPro TechEx - Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit10 Exps

    Components
    • 40 % Acrylamide (4 DegreeC)
    • APS (RT)
    • TEMED (4 DegreeC)
    • Stacking Buffer (4 DegreeC)
    • Separating Buffer (4 DegreeC)
    • Urea (RT)
    • Oligonucleotides (-20 DegreeC)
    • Sample Solubilizing buffer (RT)
    • Wash buffer (RT)
    • TE Buffer (RT)
    • Gel loading dye (RT)
    • Ethidium bromide (RT)
    • 50X TAE (RT)
    • TLC plate (with Fluorescence indicator) RT
    • Protocol

    Additional Information:

  • Item Code: OP-3108-10xp
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    DNA Estimation Teaching Kit

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    DNA Estimation Teaching Kit
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    MOQ 20 Exps

    Estimation of DNA is based on the Ethidium Bromide Fluorecent Staining of DNA. Ethidium Bromide is a Fluorescent dye, Which intercalates between the stacked bases. The uorescent yield of the dye - DNA complax is much graeter than the unbound dye. UV irradiation at 254nm is absorbed by the DNA and transmitted to the dye and the bounded dye itself absorbs radiation at 302nm and 366nm.

    CAT.NOPRODUCTEXPS
    OP-3111-5xpPro TechEx -DNA Estimation Teaching Kit5 Exps
    OP-3111-20xpPro TechEx -DNA Estimation Teaching Kit20 Exps

    Components
    • DNA sample-I (-20 DegreeC)
    • DNA sample-II (-20 DegreeC)
    • DNA sample-III (-20 DegreeC)
    • Standard DNA Solution (-20 DegreeC)
    • Agarose (RT)
    • Ethidium Bromide (RT)
    • Gel loading dye (RT)
    • 50X TAE buffer (RT)
    • Blank solution (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3111-20xp
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    Molecular Weight Determination Teaching Kit

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    Molecular Weight Determination Teaching Kit
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    DNA Molecular Weight Determination Teaching Kit.

    Agarose gel electrophoresis method is used to measure the molecular size of DNA and RNA molecules. For separation of very large DNA molecules.
    The migration rates of DNA molecule depend upon their respective molecular weight.
    This simple and fast method is frequently used to determine the size of a linear DNA fragment by comparing its mobility to a DNA fragment marker of known size.

    CAT.NOPRODUCTEXPS
    OP-3116-5xpPro TechEx-DNA Molecular Weight Determination Teaching Kit5 Exps
    OP-3116-10xpPro TechEx-DNA Molecular Weight Determination Teaching Kit10 Exps


    Components
    • 50X TAE (RT)
    • DNA sample I (4 DegreeC)
    • DNA sample II (4 DegreeC)
    • DNA marker (4 DegreeC)
    • Agarose (RT)
    • Gel loading Dye (RT)
    • Ethidium Bromide (RT)
    • Protocol

    MOQ-10 Exps

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    Additional Information:

  • Item Code: OP-3116-10xp
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    Animal Tissue Teaching Kit

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    Animal Tissue Teaching Kit
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    DNA Extraction From Animal Tissue Teaching kit.

    A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described.
    The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS.
    The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

    CAT.NOPRODUCTEXPS
    OP-3119-10xpPro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit10 Exps
    OP-3119-20xpPro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit20 Exps

    Components
    • 50X TAE (RT)
    • Agarose (RT)
    • Chloroform (RT)
    • Ethidium Bromide (RT)
    • Gel loading Dye (RT)
    • Guanidine thiocyanate (RT)
    • 70% Ethanol (RT)
    • Isopropanol (RT)
    • RNase A (-20 DegreeC)
    • TE buffer (RT)
    • DNA Marker (-20 DegreeC)
    • Protocol

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    Fungi Teaching Kit

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    Fungi Teaching Kit
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    DNA Extraction From Fungi Teaching Kit.

    We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol precipitation.
    The ethanol precipitate was then subjected to a second round of mild shearing, NaCl precipitation, chloroform extraction and ethanol precipitation.
    The procedure required approximately 1 h to perform. The method yielded 8-32 microg of high molecular weight DNA per 30 mg of freeze-dried mycelium when tested on six fungal species.

    CAT.NOPRODUCTEXPS
    OP-3122-10xpPro TechEx- Genomic DNA Extraction From Fungi Teaching Kit10 Exps
    OP-3122-20xpPro TechEx- Genomic DNA Extraction From Fungi Teaching Kit20 Exps

    Components

    • Extraction buffer (4°C)
    • CTAB (RT)
    • 5M NaCl (RT)
    • Choloroform/ Isoamyl alcohol (RT)
    • 70% Ethanol (RT)
    • TE buffer (RT)
    • Rnase (-20°C)
    • Isopropanol (RT)
    • Protocol

    20 Exp


    Additional Information:

  • Item Code: OP-3122-20xp
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    Genomic DNA Extraction Teaching Kit

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    Genomic DNA Extraction Teaching Kit
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    Genomic DNA Extraction From Yeast Teaching Kit.

    The Yeast Genomic DNA is isolated by Chloroform method. The cell wall is lysed by using lysis solution together with glass beads.
    It is followed by treatment with Phenol-Chloroform. Finally the DNA is precipitated by using ethanol. The obtained genomic DNA is resolved by using horizontal agarose gel electrophoresis.

    CAT.NOPRODUCTEXPS
    OP-3126-10xpPro TechEx-Genomic DNA Extraction From Yeast Teaching Kit10 Exps
    OP-3126-20xpPro TechEx-Genomic DNA Extraction From Yeast Teaching Kit20 Exps

    Components
    • Harju-Buffer(4 DegreeC)
    • Chloroform(4 DegreeC)
    • Precipitation solution(4 DegreeC)
    • Wash solution (RT)
    • RNase A (-20 DegreeC)
    • Yeast culture (4 DegreeC)
    • 50X TAE (RT)
    • Agarose (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • TE buffer (RT)
    • YPD (RT)
    • Protocol

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    Isolation & Characterization of Petite Mutant Teaching Kit

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    Isolation & Characterization of Petite Mutant Teaching Kit
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    MOQ 10 Exps

    Petite Mutant teaching kit


    In this method the colonies grown on glucose medium are overlay with 2, 3, 5 Triphenyl Tetrazolium chloride (TTC). After incubation the petite colonies remains white whereas the wild type colonies turned to red because of the reduction of tetrazolium chloride resulting formazan compound.

    CAT.NOPRODUCTEXPS
    OP-3129-10xpPro TechEx-Isolation and characterization of petite mutant of Yeast Teaching Kit10 Exps

    Components
    • YPD agar (RT)
    • Saccharomyces cerevisiae (4 DegreeC)
    • Tetrazoilum Chloride (4 DegreeC)
    • Protocol
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    DNA Isolation Teaching Kit

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    DNA Isolation Teaching Kit
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    Lambda Phage DNA Isolation Teaching Kit.

    Viruses that infect procaryotes are known as bacteriophages,or phages, because when they were first discovered,they appeared to eat bacterial cells, generating a clearing, or plaque, on a lawn of susceptible bacteria.
    In reality, the bacteria are killed by lysis as newly produced phages are released from the damaged cells.

    CAT.NOPRODUCTEXPS
    OP-3132-10xpPro TechEx-Lambda Phage DNA Isolation Teaching Kit10 Exps

    Components
    • 0.5MEDTA (RT)
    • 10% SDS (RT)
    • Ethyl alcohol (RT)
    • Phenol (RT)
    • TE Buffer (RT)
    • 0.01M MgSO4 (RT)
    • Chloroform (RT)
    • DNase I (-20 DegreeC)
    • Phenol: Chloroform (4 DegreeC)
    • RNAse (-20 DegreeC)
    • Maltose (20%) (4 DegreeC)
    • SM buffer (4 DegreeC)
    • NZY-amine medium (4 DegreeC)
    • Protocol

    ¿¿


    Additional Information:

  • Item Code: OP-3132-10xp
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    Ligation Teaching Kit

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    Ligation Teaching Kit
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    Joining linear DNA fragments together with covalent bonds is called ligation. More specifically, DNA ligation involves creating a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another.The enzyme used to ligate DNA fragments is T4 DNA ligase, which originates from the T4 bacteriophage. This enzyme will ligate DNA fragments having overhanging,cohesive ends that are annealed together.

    CAT.NOPRODUCTEXPS
    OP-3133-5xpPro TechEx-Ligation Teaching Kit5 Exps
    OP-3133-20xpPro TechEx-Ligation Teaching Kit20 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • Lambda EcoR I digest (-20 DegreeC)
    • Ligase Buffer (-20 DegreeC)
    • Ligase Enzyme (-20 DegreeC)
    • Sterile water (RT)
    • 0.5ml tubes (RT)
    • Protocol

    MOQ-20 Exps

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    Multiplex PCR Teaching Kit

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    Multiplex PCR Teaching Kit
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    MOQ 25 Exps

    Multiplex PCR uses several pairs of primers annealing to different target sequences. This permits the simultaneous analysis of multiple targets in a single sample. For example, in testing for genetic mutations, six or more amplifications might be combined. In the standard protocol for DNA Fingerprinting, the targets assayed are often amplified in groups of 3 or 4. Multiplex Ligation-dependent Probe Amplification (or MLPA) permits multiple targets to be amplified using only a single pair of primers, avoiding the resolution limitations of multiplex PCR. Multiplex PCR has also been used for analysis of microsatellites and SNPs.
    CAT.NOPRODUCTEXPS
    OP-3136-5xpPro TechEx-Multiplex PCR Teaching Kit5 Exps
    OP-3136-25xpPro TechEx-Multiplex PCR Teaching Kit25 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT)
    • Template DNA (-20 DegreeC)
    • Multiplex Primer(F R) (-20 DegreeC)
    • Taq Polymerase (-20 DegreeC)
    • 10 mM dNTP mix (-20 DegreeC)
    • 10X Taq polymerase Buffer (-20 DegreeC)
    • Distilled water (RT)
    • Marker DNA (-20 DegreeC)
    • PCR Tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3136-25xp
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    Mutagenesis Teaching Kit

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    Mutagenesis Teaching Kit
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    MOQ 10 Exps

    Mutation Detection and Analysis (Mutagenesis) Teaching kit.


    Mutation occur due to deletion, insertion or duplication of bases. Mutation Can be detected by restiction enzyme mapping. Insertion, deletion or duplication of a base may create a new restiction enzyme site.

    CAT.NOPRODUCTEXPS
    OP-3137-5xpPro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit5 Exps
    OP-3137-10xpPro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit10 Exps
    Components
    • Agarose (RT)
    • Control DNA (-20 DegreeC)
    • DNA (Mutated) (-20 DegreeC)
    • Ethidium Bromide (RT)
    • Restriction Enzyme (-20 DegreeC)
    • 50X TAE (RT)
    • Gel loading Dye (RT)
    • Restriction Buffer (-20 DegreeC)
    • Protocol

    Additional Information:

  • Item Code: OP-3137-10xp
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    Oligonucleotide Purification Teaching Kit

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    Oligonucleotide Purification Teaching Kit
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    Oligonucleotide synthesis involves a large number of individual reactions, which leads inevitably to the accumulation of impurities such as truncated oligonucleotides and sequences containing chemically-modified bases. These impurities must be removed after synthesis
    CAT.NOPRODUCTEXPS
    OP-3140-5xpPro TechEx-Oligonucleotide Purification Teaching Kit5 Exps
    OP-3140-10xpPro TechEx-Oligonucleotide Purification Teaching Kit10 Exps

    Components
    • 40% polyacrylamide (4 DegreeC)
    • TEMED (4 DegreeC)
    • APS (4 DegreeC)
    • Oligonucleotide I& II (-20 DegreeC)
    • Urea (RT)
    • Butanol (RT)
    • Phenol chloroform (4 DegreeC)
    • Loading buffer (4 DegreeC)
    • TE buffer (4 DegreeC)
    • Ethanol I (RT)
    • Ethanol II (RT)
    • TLC plate (RT)
    • 10X TEB (RT)
    • UV wrap (RT)
    • Protocol

    MOQ 10 Exps

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