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BIO TECH KIT
Transcriptomics is the branch of Molecular Biology which deals with study of RNA. These Transcriptomics Teaching Kits are mainly consumed in dept of Biotechnology, where Transcriptomics is consider as a part of major subject.Northern Blotting Teaching Kit
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Approx. Rs 10,000 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| I Deal In | New Only |
The term 'northern blot' actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot.
| Cat.No | Product | Experiments |
| OP-3303-5xp | Pro TechEx-Northern Blotting Teaching Kit | 5 Exps |
| Components |
| 10X Running buffer (RT) |
| Agarose (RT) |
| Formaldehyde (RT) |
| RNA Sample buffer (RT) |
| 20X SSC (RT) |
| Gel loading dye (RT) |
| RNA Sample (-20°C) |
| Protocol |
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Invitro Transcription Teaching Kit
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Approx. Rs 13,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Usage/Application | Research and institute |
| Number of Reactions(Preps)/Kit | 5xp |
| Product Type | KIT |
| Packaging Size | 5xp |
| Catalog Number | OP-3302-5xp |
| Brand | Progene |
| Country of Origin | Made in India |
In vitro transcription is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts are used in analytical techniques.
| CAT.NO | PRODUCT | EXPS |
| OP-3302-5xp | Pro TechEx-Invitro transcription Teahing Kit | 5 Exps |
Components
- DEPC treated water (-20 DegreeC)
- Murine RNase Inhibitor (-20 DegreeC)
- Ribonucleotide Solution Mix (-20 DegreeC)
- SP6 RNA Polymerase (-20 DegreeC)
- T7 RNA Polymerase Buffer (10X)(-20 DegreeC)
- Template DNA (-20 DegreeC)
- 1.5ml Microfuge Tubes (RT)
- 70% Ethanol (RT)
- Gel loading dye (RT)
- Isopropanol (RT)
- RNase H(-20 DegreeC)
- Sodium acetate (3M)(RT)
- Agarose (RT)
- Ethidium Bormide (RT)
- Phenol (RT)
- T7 RNA Polymerase (-20 DegreeC)
- TEB 10X (RT)
- Protocol
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Maxi Broad Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)
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Approx. Rs 11,540 / PieceGet Latest Price
Product Details:| Model | VBR0080 |
| Brand | Orange |
| I Deal In | New Only |
| Dimension | 55(L)x20(B)x24(H) |
| Type | Vertical Gel |
| Number of Samples | 80 |
Specification:
Make : ORANGE
Model : Regular- Maxi Broad Vertical
Cat.No : VBR0080
Dimension : 55(L)x20(B)x24(H)
Number of Samples : 80
Buffer Volume : 600 750ml
Gel Size : 41x20cm (1 Gel)
Glass Plate : 45x45cm Notched and Square Plates (2 Sets)
Number of Combs : 80-wells 1mm thickness Sharktooth comb (Well Width-3mm,Interspace-2.1mm) (1 No.)
80-wells 1.5mm thickness Sharktooth comb (Well Width-3mm,Interspace- 2.1mm) (1 No.)
40-wells 1mm thickness Sharktooth comb (Well Width-7mm,Interspace-3mm) (1 No.)
40-wells 1.5mm thickness Sharktooth comb (Well Width-7mm,Interspace-3mm) (1 No.)
Spacers : 1mm and 1.5mm Spacers (3 Nos. Each)
Gasket : Fixed
Comb Capacity -5mm Deep Well : 15ul,22ul,35ul,52ul
Clamp and Screws : 1 Set
Connecting Cord : Red and Black (Each One)
No. of Platinum electrodes : Positive and Negative (Each One)
Buffer Chamber with Safety Lid : 1 No.
Gel Casting System : 1 No.
Instruction Manual
Warranty Card
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Total RNA Extraction from Bacteria Teaching Kit
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Approx. Rs 5,000 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | RNA Extraction Kit |
| Type | Total RNA Extraction from Bacteria Teaching Kit |
| Brand | PROGENE |
| I Deal In | New Only |
| Country of Origin | Made in India |
Mid-exponential phase bacteria grown in liquid media (50-100µl pellet volume) were harvested by centrifugation at 13500 rpm for 20s in a Microfuge tube, washed in detergent solution. Repelleted and resuspended in RNase free distilled water. Re-extracted with water saturated phenol. Isolated RNA was visualized by Ethidium Bromide Stained Denaturing Formaldehyde gel electrophoresis.
| CAT.NO | PRODUCT | EXPS |
| OP-3308-5xp | Pro TechEx-Total RNA Extraction from Bacteria Teaching Kit | 5 Exps |
| OP-3308-20xp | Pro TechEx- Total RNA Extraction from Bacteria Teaching Kit | 20 Exps |
Components
- Microfuge tubes (RT)
- 10X MOPS (RT)
- 70% Ethanol (RT)
- Agarose (RT)
- E.coli Host (4 DegreeC)
- Ethidium bromide (RT)
- Gel loading dye (RT)
- Agarose (RT)
- DEPC water (RT)
- LB medium (RT)
- Solution I (4 DegreeC)
- Solution II (4 DegreeC)
- Solution III (4 DegreeC)
- Solution IV (4 DegreeC)
- Protocol
MOQ-20 Exps
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Total RNA Extraction and RT-PCR Teaching Kit
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Approx. Rs 13,500 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | RNA Extraction Kit |
| Brand | PROGENE |
| Type | PCR Application Teaching Kit |
| I Deal In | New Only |
| CAT-No | OP-3307-10xp |
| Country of Origin | Made in India |
Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. In RT-PCR, the RNA template is first converted into a complementary DNA (cDNA) using a reverse transcriptase. The cDNA is then used as a template for exponential amplification using PCR. RT-PCR is currently the most sensitive method of RNA detection available.RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.
| CAT.NO | PRODUCT | EXPS |
| OP-3307-10xp | Pro TechEx-Total RNA Extraction and RT-PCR Teaching Kit | 10 Exps |
Components
- 1.5ml Microfuge tubes (RT)
- 10mM dNTP (-20 DegreeC)
- 10X MOPS (RT)
- Agarose (RT)
- Ethidium Bromide (RT)
- Gel Loading dye (RT)
- Primer F (-20 DegreeC)
- Yeast Culture (4 DegreeC)
- 0.2ml PCR tubes (RT)
- Primer R (-20 DegreeC)
- RNase free Water (4 DegreeC)
- Solution I (4 DegreeC)
- Solution II (4 DegreeC)
- Solution III (4 DegreeC)
- Solution IV (4 DegreeC)
- Yeast broth (RT)
MOQ-10 Exp
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Total RNA Extraction From Yeast Teaching Kit
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Approx. Rs 7,000 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Packaging Size | N/A |
| Result Time | 1 day |
| Brand | PROGENE |
| I Deal In | New Only |
| Humidity Resistant | Yes |
RNA isolation is difficult procedure .Get rid of R Nases is an essential step with the help of DEPC water. The objective is to isolate a large quantity (perhaps 100 whole micrograms) of high molecular weight, undegraded total RNA. In order to further characterize the mating reaction , will be isolating total RNA from culture contains yeast cells in logarithmic growth phase grown in YPD.
| CAT.NO | PRODUCT | EXPS |
| OP-3310-5xp | Pro TechEx-Total RNA Extraction From Yeast Teaching Kit | 5 Exps |
| OP-3310-10xp | Pro TechEx-Total RNA Extraction From Yeast Teaching Kit | 10 Exps |
Components
- Ethanol I (RT)
- Ethanol II (RT)
- Isopropanol (RT)
- Phenol/chloroform (4 DegreeC)
- RNA buffer (RT)
- RNA buffer SDS (RT)
- Yeast culture (4 DegreeC)
- YPD broth (RT)
- 10X MOPS (RT)
- Agarose (RT)
- Ethidium bromide (RT)
- Formaldehyde (RT)
- Gel loading dye (RT)
- TE buffer (RT)
- Protocol
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Preparation of Poly(A) mRNA From Yeast Teaching Kit
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Approx. Rs 5,800 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Packaging Size | Box |
| Material | Plastic |
| I Deal In | New Only |
To isolate mRNA from total RNA sample, cellulose resin with a poly deoxythimidine oligomer attached (oligo-dT cellulose) is used to purify transcript with a poly-A tail. This purification should be done on the same day as the total RNA purification limit degradation. The total RNA and oligo-dT cellulose are incubated at 65°C and eluted by using elution buffer. Purified mRNA can be used for Reverse transcription process.
| Cat. NO | Product | Experiments |
| OP-3306-5xp | Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit | 5 Exps |
| OP-3306-10xp | Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit | 10 Exps |
Components:
· 10X MOPS (-20°C)
· Binding buffer (RT)
· Elution buffer (RT)
· Ethanol (RT)
· Oligo dT Cellulose (-20°C)
· Sodium acetate (RT)
· Total RNA (-20°C)
· 70% ethanol (RT)
· Agarose (RT)
· DEPC water (RT)
· Ethidium Bromide (RT)
· Formaldehyde (RT)
· Gel loading Dye (RT)
· Protocol
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Total Rna Isolation From Yeast By Trizol Method
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Approx. Rs 5,800 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Shelf Life | 12 Months |
| Form | Liquid |
| Accuracy | 0.99 |
| I Deal In | New Only |
| Humidity Resistant | Yes |
This protocol uses Trizol (also known as TRI REAGENT) for the isolation of total RNA. Trizol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, RNA and protein on homogenization or lysis of tissue sample. After adding chloroform and centrifuging, the mixture separates into 3 phases with the upper clear aqueous phase containing the RNA. The next steps in the extraction are washes and precipitation of the RNA.
| CAT.NO | PRODUCT | EXPS |
| OP-3309-5xp | Pro TechEx-Total RNA isolation from by Trizol method Teaching Kit | 5 Exps |
| OP-3309-10xp | Pro TechEx-Total RNA isolation from by Trizol method Teaching Kit | 10 Exps |
Components
- Chloroform (RT)
- TE buffer (RT)
- Trizol (4 DegreeC)
- Wash solution (RT)
- Yeast culture (4 DegreeC)
- 10X MOPS (RT)
- Agarose (RT)
- Ethidium bromide (RT)
- Formaldehyde (RT)
- Gel loading dye (RT)
- Protocol
Additional Information:
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Plant RNA Isolation Teaching Kit
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Approx. Rs 5,000 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | RNA Extraction Kit |
| Accuracy | 0.99 |
| Type | Plant RNA Isolation Teaching Kit |
| I Deal In | New Only |
| Country of Origin | Made in India |
Isolation of pure, under graded total RNA from plant tissue can be difficult because of high levels of ribonucleases, pectins and polyphenols that are present in plant material. Another major problem is the presence and removal of proteins. This problem is commonly solved by lysing the plant cells in the presence of phenol/chloroform.
| CAT.NO | PRODUCT | EXPS |
| OP-3305-5xp | Pro TechEx - Plant RNA Isolation Teaching Kit | 5 Exps |
| OP-3305-10xp | Pro TechEx- Plant RNA Isolation Teaching Kit | 10 Exps |
Components
- 10X MOPS buffer (RT)
- Agarose (RT)
- Chloroform Solution (4°C)
- Ethidium Bromide (RT)
- Extraction buffer (4°C)
- Gel loading Dye (RT)
- Phenol:Chloroform Solution (4°C)
- Sodium acetate pH 5.2 (RT)
- 70% ethanol (RT)
- Absolute ethanol (4°C)
- DEPC-treated water (RT)
- Formaldehyde (RT)
- Marker (RT)
- Protocol
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