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BIOTECH KITS
Molecular Biology- Genomics:
Molecular biologyis the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas ofbiologyandchemistry, particularlygeneticsandbiochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of acell, including the interactions between the different types ofDNA,RNAandprotein biosynthesisas well as learning how these interactions are regulated.
Chloroplast DNA Isolation Teaching Kit
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Approx. Rs 11,900 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| ICMR Approved | No |
| Brand | PROGENE |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 10 |
| Shelf Life | 12 Months |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3105-10xp |
| Country of Origin | Made in India |
In all land plants and algae, chloroplast DNA (cpDNA) has been found to exist as a single circular molecules ranging from 80 to 300 kb. The chloroplast genome is densely packed with genes. Chloroplast gene products identifies function either in photosynthesis or as components of the chloroplast protein synthesizing system.In this procedure, sucrose step gradient procedure is used to isolate chloroplast and cpDNA.
Cat. NO | Product | Experiments |
OP-3105-10xp | Pro TechEx - Chloroplast DNA Isolation Teaching Kit | 10 Exps |
Components:
STE Buffer (4°C)
BSA (4°C)
BME (4°C)
ST Buffer (4°C)
TE Saline Buffer (4°C)
Isopropanol (RT)
Tris saturated phenol (4°C)
Phenol/Chloroform (4°C)
Chloroform /Isoamyl alcohol (4°C)
Magnesium acetate (RT)
Ammonium acetate (RT)
Dnase I (-20°C)
Potassium acetate (RT)
SDS (RT)
EDTA (RT)
Ethanol I (RT)
Ethanol II (RT)
Protocol
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Genomic DNA Extraction From Whole Blood Teaching Kit
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Approx. Rs 3,800 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | Progene |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 10 |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| Packaging Size | 10 |
| I Deal In | New Only |
The Genomic DNA is Isolated from Whole Blood by using RBCL solution which lysis the RBC and WBC left intact. The white blood cells and their nuclear membrane are lysis by WBCL solution then the proteins and other cellular components are removed by saturation solution. Finally the high molecular weight Genomic DNA is precipitated by using absolute alcohol. The obtained Genomic DNA is resolved by using Agarose gel electrophoresis.
| Cat. NO | Product | Experiments |
| OP-3125-10xp | Pro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit | 10 Exps |
| OP-3125-20xp | Pro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit | 20 Exps |
Components:
RCLB (RT)
100% Ethanol (RT)
50X TAE (RT)
70% Ethanol (RT)
Agarose (RT)
Ethidium Bromide (RT)
WCLB (RT)
Gel Loading Dye (RT)
DNA Marker (-20°C)
Microfuge Tubes (RT)
Saturation solution (RT)
TE Buffer (RT)
Protocol
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Lambda Phage DNA Isolation Teaching Kit
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Approx. Rs 6,900 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | Progene |
| Usage/Application | Hospital |
| Form | Kit |
| Method | Lambda |
| I Deal In | New Only |
| Humidity Resistant | Yes |
Lambda Phage DNA Isolation Teaching Kit.
Viruses that infect procaryotes are known as bacteriophages,or phages, because when they were first discovered,they appeared to eat bacterial cells, generating a clearing, or plaque, on a lawn of susceptible bacteria.
In reality, the bacteria are killed by lysis as newly produced phages are released from the damaged cells.
| CAT.NO | PRODUCT | EXPS |
| OP-3132-10xp | Pro TechEx-Lambda Phage DNA Isolation Teaching Kit | 10 Exps |
Components
- 0.5MEDTA (RT)
- 10% SDS (RT)
- Ethyl alcohol (RT)
- Phenol (RT)
- TE Buffer (RT)
- 0.01M MgSO4 (RT)
- Chloroform (RT)
- DNase I (-20 DegreeC)
- Phenol: Chloroform (4 DegreeC)
- RNAse (-20 DegreeC)
- Maltose (20%) (4 DegreeC)
- SM buffer (4 DegreeC)
- NZY-amine medium (4 DegreeC)
- Protocol
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Transduction Teaching Kit
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Approx. Rs 4,350 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Brand | PROGENE |
| I Deal In | New Only |
| Type | Transduction Teaching Kit |
| Country of Origin | Made in India |
Transduction is the process by which DNA is transferred from one bacterium to another by a virus. It also refers to the process whereby foreign DNA is introduced into another cell via a viral vector. Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation), and it is DNase resistant (transformation is susceptible to DNase). Transduction is a common tool used by molecular biologists to stably introduce a foreign gene into a host cell's genome.
| CAT.NO | PRODUCT | EXPS |
| OP-3163-5xp | Pro TechEx-Transduction Teaching Kit | 5 Exps |
| OP-3163-20xp | Pro TechEx-Transduction Teaching Kit | 20 Exps |
- (4 DegreeC)
- Donar strain (-20 DegreeC)
- Host strain (-20 DegreeC)
- (4 DegreeC)
- Phage lysate (-20 DegreeC)
- Recipient strain (-20 DegreeC)
- 10% Dextrose (4 DegreeC)
- 1M Calcium chloride (RT)
- 1M Magnesium chloride (RT)
- Agar (RT)
- LB Broth (RT)
- Protocol
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Southern Blotting Teaching Kit
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Approx. Rs 7,500 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Research and institute |
| Model Number | OP-3158 |
| Brand | PROGENE |
| Type | Southern Blotting Teaching Kit |
| I Deal In | New Only |
| Country of Origin | Made in India |
Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1975. It allows investigators to determine the molecular weight of a restriction fragment, to measure relative amounts in different samples and to locate a particular sequence of DNA within a complex mixture. DNA (genomic or other source) is digested with a restriction enzyme and separated by gel electrophoresis and transferred from an agarose gel onto a membrane.
| CAT.NO | PRODUCT | EXPS |
| OP-3158-5xp | Pro TechEx-Southern Blotting Teaching Kit | 5 Exps |
| OP-3158-20xp | Pro TechEx-Southern Blotting Teaching Kit | 20 Exps |
Components
- 50X TAE (RT)
- Agarose (RT)
- DNA sample (-20 DegreeC)
- Ethidium Bromide (RT)
- Gel loading dye (RT)
- 20X SSC (RT)
- Nitrocellulose Membrane (RT)
- Protocol
MOQ-20 Exps
Additional Information:
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Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit
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Approx. Rs 3,300 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| I Deal In | New Only |
The kit contains a membrane embedded in a spin column for binding up to 1-5ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR and other molecular technique.
| Cat. NO | Product | Experiments |
| OP-3127-10xp | Por TechEx-Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit | 10 Exps |
| OP-3127-20xp | Por TechEx-Genomic DNA Purification By Using DNA Binding Membrane Teaching Kit | 20 Exps |
Components:
50X TAE (RT)
Agarose (RT)
column with 2ml collection
tube (RT)
Elution buffer (4°C)
Ethidium bromide (RT)
Gel loading dye (RT)
Lysis buffer (4°C)
TE Buffer (RT)
Wash buffer (RT)
Protocol
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Phage Titration Teaching Kit
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Approx. Rs 5,200 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Brand | PROGENE |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| Shelf Life | 12 Months |
| I Deal In | New Only |
| Humidity Resistant | Yes |
The number of phage particles contained in the original stock phage culture is determined by counting the number of plaques formed on the seeded agar plate and multiplying this by the dilution factor. For a valid phage count, the number of plaques per plate should not exceed 300 nor be less than 30. Plates showing greater than 300 PFUs are too numerous to count (TNTC); plates showing fewer than 30 PFUs are too few to count (TFTC).
| OP-3145-5xp | Pro TechEx-Phage Titration Teaching Kit | 5 Exps |
| OP-3145-20xp | Pro TechEx-Phage Titration Teaching Kit | 20 Exps |
Components:
10X SM Buffer (RT)
20% Maltose solution (RT)
Agar (1.5 g /100 ml) (RT)
Sodium Chloride (RT)
Tryptone (RT)
20% Magnesium Chloride (RT)
E.coli Host (4°C)
LB broth (RT)
Phage lysate (4°C)
Protocol
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Protoplast Fusion Teaching Kit
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Approx. Rs 2,650 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| Brand | PROGENE |
| I Deal In | New Only |
An improved procedure has been developed for the fusion of plant protoplasts based on the use of polyethylene glycol possessing a low carbonyl content. Different commercial preparations of PEG were analysed using a sensitive carbonyl assay procedure and only those with a very low carbonyl content (< 5 × 10−4M) were used. Sterilisation by membrane filtration was employed, since autoclaving markedly increased the carbonyl content with associated deleterious effects on fusion frequency and heterokaryon viability. Elution of PEG was carried out using a hypotonic mannitol solution at pH 5.8, without added Ca2+, immediately followed by 9 % w/v mannitol (pH 5.8), again without added Ca2+. This improved PEG fusion procedure has been found to be applicable to a range of protoplast combinations. Its use results in a high fusion frequency with good resultant heterokaryon viability and a low level of clumping.
| Cat. NO | Product | Experiments |
| OP-3149-5xp | Protech Ex - Protoplast fusion Teaching kit | 5 Exps |
Components:
70% ethanol (RT)
Holding solution (RT)
Enzyme digest solution (4°C)
Dilution solution I (RT)
Dilution solution II (RT)
Agglutination solution (RT)
Glass slide (RT)
Cover slips (RT)
1.5ml centrifuge tubes (RT)
Protocol
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Rapid Plasmid Screening Teaching Kit
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Approx. Rs 3,000 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Material | Plastic |
| Shelf Life | 12 Months |
| I Deal In | New Only |
| Humidity Resistant | Yes |
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis.
| Cat. NO | Product | Experiments |
| OP-3150-5xp | Pro TechEx-Rapid Plasmid Screening Teaching Kit | 5 Exps |
| OP-3150-20xp | Pro TechEx-Rapid Plasmid Screening Teaching Kit | 20 Exps |
Components:
E.coli host (4°C)
50X TAE (RT)
6X gel loading dye (RT)
Agar (RT)
Agarose (RT)
LB-Broth (RT)
Ampicillin (4°C)
Ethidium Bromide (RT)
Microfuge tube (RT)
Solution I (4°C)
Solution II (4°C)
Protocol
Additional Information:
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Genomic DNA Extraction From Bacteria Teaching Kit
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Approx. Rs 3,850 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Number of Reactions(Preps)/Kit | 10xp |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3121-10xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Many different methods and technologies are available for the isolation of genomic DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.
| CAT.NO | PRODUCT | EXPS |
| OP-3121-10xp | Pro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit | 10 Exps |
| OP-3121-20xp | Pro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit | 20 Exps |
- E.coli strain (4 DegreeC)
- 50X TAE (RT)
- LB media (RT)
- Phenol/Chloroform (2524) (RT)
- Proteinase K (4 DegreeC)
- SDS (RT)
- TE Buffer (RT)
- 3M Sodium acetate pH 5.2 (RT)
- Agarose (RT)
- DNA Marker (-20 DegreeC)
- Ethanol (70%) (RT)
- Ethidium Bromide (RT)
- Gel loading dye (RT)
- Isopropanol (100%) (RT)
- Protocol
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Mitochondrial DNA Isolation Teaching Kit
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Approx. Rs 11,900 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | PROGENE |
| Shelf Life | 12 Months |
| Number of Reactions(Preps)/Kit | 10 |
| Accuracy | 0.99 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3135-10xp |
| Country of Origin | Made in India |
Mitochondrial DNA Isolation Teaching Kit.
Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus, and in plants, the chloroplast as well.
In humans, mitochondrial DNA can be assessed as the smallest chromosome coding for 37 genes and containing approximately 16,600 base pairs.Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria; cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, triphosphate (ATP).
Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus, and in plants, the chloroplast as well.
| CAT.NO | PRODUCT | EXPS |
| OP-3135-10xp | Pro TechEx-Mitochondrial DNA Isolation Teaching Kit | 10 Exps |
Components
- BME(4 DegreeC)
- BSA(4 DegreeC)
- Isopropanol (RT)
- ST Buffer (4 DegreeC)
- STE Buffer (4 DegreeC)
- TE Saline Buffer (4 DegreeC)
- Ammonium acetate (RT)
- Chloroform /Isoamyl alcohol (4 DegreeC)
- DNase I (-20 DegreeC)
- Magnesium acetate (RT)
- Phenol/Chloroform (4 DegreeC)
- Tris saturated phenol (4 DegreeC)
- Ethanol II (RT)
- EDTA (RT)
- Ethanol I (RT)
- SDS (RT)
- Potassium acetate (RT)
- Protocol
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Restriction Digestion Teaching Kit
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Approx. Rs 4,050 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| I Deal In | New Only |
Restriction enzymes are Nucleases which can cleave the sugar-phosphate backbone of DNA, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They specifically cleave the nucleic acids at specific nucleotide sequence called Restriction sites to generate a set of smaller fragments.
| Cat. NO | Product | Experiments |
| OP-3153-5xp | Pro TechEx-Restriction Digestion Teaching Kit | 5 Exps |
| OP-3153-20xp | Pro TechEx-Restriction Digestion Teaching Kit | 20 Exps |
| Components |
| 50X TAE (RT) |
| Agarose (RT) |
| Ethidium bromide (RT) |
| Gel loading dye (RT) |
| Template DNA (-20°C) |
| 0.5ml tubes (RT) |
| Enzyme Buffer (-20°C) |
| Hind III (-20°C) |
| Sterile water (RT) |
| Protocol |
Additional Information:
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Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit
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Approx. Rs 6,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3137-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Nucleosome Mapping by Micrococcal Nulease (MNase).
The basic repeating unit of chromatin, the nucleosome, is known to play a critical role in regulating the process of gene transcription.
The positioning of nucleosomes on a promoter is a significant determinant in its responsiveness to gene-inducing signals. For example, positioning and subsequent mobilization of nucleosomes can regulate the access of various DNA factors to underlying DNA templates.
Several mechanisms have been proposed to direct the process of nucleosome displacement such as chemical histone modifications, ATP-dependent remodelling, and the incorporation of histone variants.
Thus, rather than being an inert molecular structure, chromatin is highly dynamic in response to the transcription process. In this section, we describe two methodologies that allow the determination of exact nucleosome positioning within specific gene regions.
| CAT.NO | PRODUCT | EXPS |
| OP-3139-5xp | Pro TechEx-Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit | 5 Exps |
| OP-3139-20xp | Pro TechEx-Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit | 20 Exps |
Components
- 2X TNSEK solution (RT)
- Nuclear Buffer I (-20 DegreeC)
- Nuclear Buffer II (-20 DegreeC)
- Nuclear Buffer III (-20 DegreeC)
- PBS (RT)
- 0.1M CaCl2 (4 DegreeC)
- MNase (-20 DegreeC)
- NaOH 1M (RT)
- Protocol
MOQ-20 Exps
Additional Information:
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Gel Extraction Teaching Kit
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Approx. Rs 4,400 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3117-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps. This process, usually performed on plasmids.After DNA samples are run on an agarose gel, extraction involves four basic steps identifying the fragments of interest, isolating the corresponding bands, isolating the DNA from those bands, and removing the accompanying salts and stain.
| CAT.NO | PRODUCT | EXPS |
| OP-3117-5xp | Pro TechEx-Gel Extraction Teaching Kit | 5 Exps |
| OP-3117-20xp | Pro TechEx-Gel Extraction Teaching Kit | 20 Exps |
Components
- 50X TAE (RT)
- Ethidium bromide (RT)
- Gel loading dye (RT)
- Silica powder (RT)
- Solubilizing buffer (RT)
- TE Buffer (RT)
- Wash buffer (RT)
- DNA sample (-20 Degree)
- DNA marker (-20 Degree)
- Agarose (RT)
- Protocol
Additional Information:
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DNA Estimation Teaching Kit
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Approx. Rs 5,300 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 10 |
| Packaging Size | 10 |
| Catalog Number | OP-3111-10xp |
| Brand | PROGENE |
Estimation of DNA is based on the Ethidium Bromide Fluorecent Staining of DNA. Ethidium Bromide is a Fluorescent dye, Which intercalates between the stacked bases. The uorescent yield of the dye - DNA complax is much graeter than the unbound dye. UV irradiation at 254nm is absorbed by the DNA and transmitted to the dye and the bounded dye itself absorbs radiation at 302nm and 366nm.
| CAT.NO | PRODUCT | EXPS |
| OP-3111-10xp | Pro TechEx -DNA Estimation Teaching Kit | 10 Exps |
| OP-3111-20xp | Pro TechEx -DNA Estimation Teaching Kit | 20 Exps |
Components
- DNA sample-I (-20 DegreeC)
- DNA sample-II (-20 DegreeC)
- DNA sample-III (-20 DegreeC)
- Standard DNA Solution (-20 DegreeC)
- Agarose (RT)
- Ethidium Bromide (RT)
- Gel loading dye (RT)
- 50X TAE buffer (RT)
- Blank solution (RT)
- Protocol
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Mutagenesis Teaching Kit
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Approx. Rs 5,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3137-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Mutation Detection and Analysis (Mutagenesis) Teaching kit.
Mutation occur due to deletion, insertion or duplication of bases. Mutation Can be detected by restiction enzyme mapping. Insertion, deletion or duplication of a base may create a new restiction enzyme site.
| CAT.NO | PRODUCT | EXPS |
| OP-3137-5xp | Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit | 5 Exps |
| OP-3137-10xp | Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit | 10 Exps |
- Agarose (RT)
- Control DNA (-20 DegreeC)
- DNA (Mutated) (-20 DegreeC)
- Ethidium Bromide (RT)
- Restriction Enzyme (-20 DegreeC)
- 50X TAE (RT)
- Gel loading Dye (RT)
- Restriction Buffer (-20 DegreeC)
- Protocol
Additional Information:
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Oligonucleotide Purification Teaching Kit
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Approx. Rs 8,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3140-5xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Oligonucleotide synthesis involves a large number of individual reactions, which leads inevitably to the accumulation of impurities such as truncated oligonucleotides and sequences containing chemically-modified bases. These impurities must be removed after synthesis
| CAT.NO | PRODUCT | EXPS |
| OP-3140-5xp | Pro TechEx-Oligonucleotide Purification Teaching Kit | 5 Exps |
| OP-3140-10xp | Pro TechEx-Oligonucleotide Purification Teaching Kit | 10 Exps |
Components
- 40% polyacrylamide (4 DegreeC)
- TEMED (4 DegreeC)
- APS (4 DegreeC)
- Oligonucleotide I& II (-20 DegreeC)
- Urea (RT)
- Butanol (RT)
- Phenol chloroform (4 DegreeC)
- Loading buffer (4 DegreeC)
- TE buffer (4 DegreeC)
- Ethanol I (RT)
- Ethanol II (RT)
- TLC plate (RT)
- 10X TEB (RT)
- UV wrap (RT)
- Protocol
MOQ 10 Exps
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Plasmid Purification By DNA Binding Membrane Teaching Kit
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Approx. Rs 3,700 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Usage/Application | Research and institute |
| Number of Reactions(Preps)/Kit | 10xp |
| Product Type | KIT |
| Packaging Size | 10xp |
| Catalog Number | OP-3148-10xp |
| Brand | progene |
| Country of Origin | Made in India |
After harvesting and resuspension, the bacterial cells are lysed in NaOH-SDS in the presence of RNase A. SDS solubilizes the phospholipid and protein components of the cell membrane, leading to lysis and release of the cell contents. NaOH denatures the chromosomal and plasmid DNAs, as well as proteins. The optimized lysis time allows maximum release of plasmid DNA from the cell without release of cell wall-bound chromosomal DNA, while minimizing the exposure of the plasmid to denaturing conditions. Long exposure to alkaline conditions may cause the plasmid to become irreversibly denatured. This denatured form of the plasmid runs faster on agarose gels and is resistant to restriction enzyme digestion. The lysate is neutralized by the addition of acidic potassium acetate. The high salt concentration causes KDS* to precipitate, and the denatured proteins, chromosomal DNA, and cellular debris become trapped in salt-detergent complexes.
| CAT.NO | PRODUCT | EXPS |
| OP-3148-10xp | Pro TechEx- Plasmid Purification By DNA Binding Membrane Teaching Kit | 10 Exps |
- 2ml Collection Vial (RT)
- Column (RT)
- Elution Buffer (RT)
- Gel loading dye (RT)
- Solution I (RT)
- Solution II (RT)
- Solution III (RT)
- Wash Solution (RT)
- 50X TAE (RT)
- Agarose (RT)
- Ampicillin_ (4 DegreeC)
- DNA Marker (-20 DegreeC)
- E.coli Host (4 DegreeC)
- Ethidium Bromide (RT)
- RNase A (-20 DegreeC)
- Protocol
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Ligation Teaching Kit
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Approx. Rs 3,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| Brand | PROGENE |
| I Deal In | New Only |
Joining linear DNA fragments together with covalent bonds is called ligation. More specifically, DNA ligation involves creating a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another.The enzyme used to ligate DNA fragments is T4 DNA ligase, which originates from the T4 bacteriophage. This enzyme will ligate DNA fragments having overhanging,cohesive ends that are annealed together.
| CAT.NO | PRODUCT | EXPS |
| OP-3133-5xp | Pro TechEx-Ligation Teaching Kit | 5 Exps |
| OP-3133-20xp | Pro TechEx-Ligation Teaching Kit | 20 Exps |
Components
- Agarose (RT)
- 50X TAE (RT)
- Ethidium bromide (RT)
- Gel loading dye (RT)
- Lambda EcoR I digest (-20 DegreeC)
- Ligase Buffer (-20 DegreeC)
- Ligase Enzyme (-20 DegreeC)
- Sterile water (RT)
- 0.5ml tubes (RT)
- Protocol
MOQ-20 Exps
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Genomic DNA Extraction From Yeast Teaching Kit
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Approx. Rs 4,400 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | PROGENE |
| Shelf Life | 12 Months |
| Number of Reactions(Preps)/Kit | 10 |
| Accuracy | 0.99 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3126-10 |
| Country of Origin | Made in India |
Genomic DNA Extraction From Yeast Teaching Kit.
The Yeast Genomic DNA is isolated by Chloroform method. The cell wall is lysed by using lysis solution together with glass beads.
It is followed by treatment with Phenol-Chloroform. Finally the DNA is precipitated by using ethanol. The obtained genomic DNA is resolved by using horizontal agarose gel electrophoresis.
| CAT.NO | PRODUCT | EXPS |
| OP-3126-10xp | Pro TechEx-Genomic DNA Extraction From Yeast Teaching Kit | 10 Exps |
| OP-3126-20xp | Pro TechEx-Genomic DNA Extraction From Yeast Teaching Kit | 20 Exps |
Components
- Harju-Buffer(4 DegreeC)
- Chloroform(4 DegreeC)
- Precipitation solution(4 DegreeC)
- Wash solution (RT)
- RNase A (-20 DegreeC)
- Yeast culture (4 DegreeC)
- 50X TAE (RT)
- Agarose (RT)
- Ethidium bromide (RT)
- Gel loading dye (RT)
- TE buffer (RT)
- YPD (RT)
- Protocol
Additional Information:
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Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit
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Approx. Rs 6,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Number of Reactions(Preps)/Kit | 5xp |
| Product Type | KIT |
| Packaging Size | 5xp |
| Catalog Number | OP-3162-5xp |
| Brand | Progene |
Physical and Chemical Mutagens on E.coli teaching kit Pro Tech Ex-Study of Physical and Chemical Mutagens on The Su UV Light.
UV light which has a lower energy content than ionizing radiations, are capable of producing lethal effects in cells exposed to low penetrating wavelengths in range of 210 to 300nm.
Cellular components capable of absorbiong UV light are nucleic acids with the DNA as the primary site of damage. this results in the inabilityy to form viable cells after exposure to UV radiation.
Hydroxylamine is used to mutagenize DNA in Vitro, when used in vitro, Hydroxylamine reacts with cytosine, converting it to a modified base that pairs with adenine.
This has two consequences that is Hydroxylamine only produces GC to AT transitions, and mutations induced by hydroxylamine cannot be reverted with hydroxylamine.
| CAT.NO | PRODUCT | EXPS |
| OP-3162-5xp | Pro TechEx-Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit | 5 Exps |
Components
- Agar (RT)
- E.coli host (4 DegreeC)
- Hydroxylamine (RT)
- LB broth (RT)
- Saline (RT)
- sodium hydroxide (RT)
- Protocol
Additional Information:
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Genomic DNA Extraction From Animal Tissue Teaching Kit
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Approx. Rs 3,850 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 10 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3119-10xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
DNA Extraction From Animal Tissue Teaching kit.
A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described.
The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS.
The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.
| CAT.NO | PRODUCT | EXPS |
| OP-3119-10xp | Pro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit | 10 Exps |
| OP-3119-20xp | Pro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit | 20 Exps |
Components
- 50X TAE (RT)
- Agarose (RT)
- Chloroform (RT)
- Ethidium Bromide (RT)
- Gel loading Dye (RT)
- Guanidine thiocyanate (RT)
- 70% Ethanol (RT)
- Isopropanol (RT)
- RNase A (-20 DegreeC)
- TE buffer (RT)
- DNA Marker (-20 DegreeC)
- Protocol
Additional Information:
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Study of DNA Methylation Teaching Kit
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Approx. Rs 7,500 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| ICMR Approved | No |
| Number of Reactions(Preps)/Kit | 5xp |
| Packaging Size | 5xp |
| Catalog Number | OP-3160-5xp |
| Brand | Progene |
DNA methylation is a biochemical process where a methyl group is added to the cytosine or adenine DNA nucleotides.
| CAT.NO | PRODUCT | EXPS |
| OP-3160-5xp | Pro TechEx-Study of DNA Methylation Teaching Kit | 5 Exps |
Components
- EcoRI Methylase Enzyme (-20 DegreeC)
- 10X Restriction Buffer (-20 DegreeC)
- Lambda DNA (-20 DegreeC)
- Methylase Buffer (10X) (-20 DegreeC)
- Restriction enzyme (EcoRI) (-20 DegreeC)
- S-Adenosyl methionine (SAM) (-20 DegreeC)
- 50X TAE (RT)
- Agarose (RT)
- Distilled Water(-20 DegreeC)
- Ethidium Bromide (RT)
- Gel loading Dye (RT)
- Microfuge Tubes (RT)
- Protocol
Additional Information:
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PCR Amplification of Beta Galactosidase Gene Teaching Kit
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Approx. Rs 6,550 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3142-5xp |
| Model Number | OP-3142-5xp |
| Brand | Progene |
The size of pUC19 will be increased to 5755bp with the addition of lacZ gene, which encodes β-galactosidase. The lacZ gene was amplified by PCR method. The amplified sequence was transformed to E.coli host .The production of β-galactosidase enzyme was analysed by SDS - PAGE method.
| CAT.NO | PRODUCT | EXPS |
| OP-3142-5xp | Pro TechEx-PCR Amplification of β-Galactosidase Gene Teaching Kit | 5 Exps |
Components
- Agarose (RT)
- 50X TAE (RT)
- Ethidium Bromide (RT)
- Gel Loading Dye (RT)
- Template DNA (-20 DegreeC)
- Primer (-20 DegreeC)
- Taq Polymerase (-20 DegreeC)
- 10 mM dNTP mix (-20 DegreeC)
- 10X Taq polymerase Buffer (-20 DegreeC)
- Distilled water (RT)
- Marker DNA (-20 DegreeC)
- PCR Tubes (RT)
- Protocol
20 Exp
Additional Information:
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Competent Cell Preparation Teaching Kit
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Approx. Rs 7,000 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Brand | Progene |
| Number of Reactions(Preps)/Kit | 10 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3107-10xp |
| Country of Origin | Made in India |
There are several ways to prepare competent cells for plasmid DNA transformation. This is the chemical method(CaCl2). Advantages are that it's simple to complete, requires no special equipment and gives good transformation ef ciencies.
| CAT.NO | PRODUCT | EXPS |
| OP-3107-10xp | Pro TechEx -Competent Cell Preparation Teaching Kit | 10 Exps |
| OP-3107-20xp | Pro TechEx -Competent Cell Preparation Teaching Kit | 20 Exps |
- E.coli Host (4 DegreeC)
- Solution A (CaCl2) (4 DegreeC)
- Agar (RT)
- LB Broth (RT)
- Glycerol (RT)
- Protocol
20 Exp
Additional Information:
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Southern Hybridization Teaching Kit
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Approx. Rs 9,300 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 5 |
| Product Type | KIT |
| Packaging Size | 5 |
| Catalog Number | OP-3159 |
| Brand | PROGENE |
| Country of Origin | Made in India |
Hybridization involves several steps. First, the probe and sample DNA are allowed to hybridize under the appropriate conditions. The correct temperature, incubation times, and buffer conditions must be used. Next, the sample DNA is washed using conditions that will remove unhybridized probe but not the hybridized probe. Finally, the sample DNA is tested for the presence of the hybridized probe. The probe is labeled with a radioactive molecular tag (or some other tag) that allows it to be detected following hybridization.
| CAT.NO | PRODUCT | EXPS |
| OP-3159-5xp | Pro TechEx-Southern Hybridization Teaching Kit | 5 Exps |
Components
- 10X Safe Blue DNA Stain (-20 DegreeC)
- 50X TAE (RT)
- Agarose (RT)
- Control DNA (RT)
- DNA Sample I (RT)
- DNA Sample II (RT)
- EthidiumBromide(RT)
- 10X Substrate (RT)
- 5X Wash Buffer-A (RT)
- 5X Wash Buffer-B (RT)
- Biotinylated Probe (RT)
- HRP-Conjugate (RT)
- Hybridization Buffer (RT)
- Prehybridization Buffer (RT)
- 50X Electrotransfer Buffer (RT)
- 5X Blocking Buffer (RT)
- 5X Wash Buffer-C (RT)
- Blocking Powder (RT)
- Casein (RT)
- Control Nylon Membrane (RT)
- Nylon Membrane (RT)
- Protocol
Additional Information:
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Single Nucleotide Polymorphisms Teaching Kit
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Approx. Rs 5,200 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 5 Piece |
| Brand | progene |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 5xp |
| Product Type | KIT |
| Packaging Size | 5xp |
| Catalog Number | OP-3156-5xp |
| Country of Origin | Made in India |
Genetic disorders and other related diseases are often associated with Single Nucleotide Polymorphism (SNP) occurring at precise positions on genes/ genomes. Development of SNP-specific primers that amplify a specific fragment in PCR when a specific SNP is present helps in disease detection.SNP detection where two DNA templates, one wild-type and the other template with SNP are used for PCR amplification individually along with 2 pairs (4 numbers) of primers, one pair specific to wild-type and the other, specific to SNP-type primer.
| CAT.NO | PRODUCT | EXPS |
| OP-3156-5xp | Pro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit | 5 Exps |
| OP-3156-20xp | Pro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit | 20 Exps |
- Control DNA (-20 DegreeC)
- DNA Marker (-20 DegreeC)
- dNTP Mix (-20 DegreeC)
- Primer Mix* (-20 DegreeC)
- SNP DNA (-20 DegreeC)
- Taq DNA Polymerase(-20 DegreeC)
- Taq DNA Polymerase
- Buffer 10X (-20 DegreeC)
- 0.2ml PCR Tube (RT)
- 50X TAE (RT)
- Agarose (RT)
- Distilled water (RT)
- Ethidium Bromide (RT)
- Gel loading Dye (RT)
- Protocol
Additional Information:
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Isolation & Characterization of Petite Mutant Teaching Kit
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Approx. Rs 5,400 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Usage/Application | Laboratory |
| Number of Reactions(Preps)/Kit | 10 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3129-10xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
Petite Mutant teaching kit
In this method the colonies grown on glucose medium are overlay with 2, 3, 5 Triphenyl Tetrazolium chloride (TTC). After incubation the petite colonies remains white whereas the wild type colonies turned to red because of the reduction of tetrazolium chloride resulting formazan compound.
| CAT.NO | PRODUCT | EXPS |
| OP-3129-10xp | Pro TechEx-Isolation and characterization of petite mutant of Yeast Teaching Kit | 10 Exps |
Components
- YPD agar (RT)
- Saccharomyces cerevisiae (4 DegreeC)
- Tetrazoilum Chloride (4 DegreeC)
- Protocol
Additional Information:
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DNA Molecular Weight Determination Teaching Kit
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Approx. Rs 4,200 / PieceGet Latest Price
Product Details:| Minimum Order Quantity | 10 Piece |
| Number of Reactions(Preps)/Kit | 10 |
| Product Type | KIT |
| Packaging Size | 10 |
| Catalog Number | OP-3116-10xp |
| Brand | PROGENE |
| Country of Origin | Made in India |
DNA Molecular Weight Determination Teaching Kit.
Agarose gel electrophoresis method is used to measure the molecular size of DNA and RNA molecules. For separation of very large DNA molecules.
The migration rates of DNA molecule depend upon their respective molecular weight.
This simple and fast method is frequently used to determine the size of a linear DNA fragment by comparing its mobility to a DNA fragment marker of known size.
| CAT.NO | PRODUCT | EXPS |
| OP-3116-5xp | Pro TechEx-DNA Molecular Weight Determination Teaching Kit | 5 Exps |
| OP-3116-10xp | Pro TechEx-DNA Molecular Weight Determination Teaching Kit | 10 Exps |
Components
- 50X TAE (RT)
- DNA sample I (4 DegreeC)
- DNA sample II (4 DegreeC)
- DNA marker (4 DegreeC)
- Agarose (RT)
- Gel loading Dye (RT)
- Ethidium Bromide (RT)
- Protocol
MOQ-10 Exps
Additional Information:
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Bacterial Gene Expression Teaching Kit
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Approx. Rs 7,200 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Brand | PROGENE |
| I Deal In | New Only |
| Type | Bacterial Gene Expression Teaching Kit |
| Portable | Yes |
| Country of Origin | Made in India |
Bacterial Gene expression is the process by which information from a gene is used to the synthesis a functional gene product.
| CAT.NO | PRODUCT | EXPS |
| OP-3104-5xp | Pro TechEx - Bacterial Gene Expression Teaching Kit | 5 Exps |
Components
| Recombinant bacterial culture (4 DegreeC) | APS (RT) |
| Inducer(IPTG) (4 DegreeC) | Sample Solubilizing buffer (4 DegreeC) |
| Ampicillin (4 DegreeC) | Running gel buffer mix (RT) |
| LB broth powder (RT) | Stainer (RT) |
| Separating gel mix (4 DegreeC) | Destainer (RT) |
| Stacking gel mix (4 DegreeC) | Protocol |
Additional Information:
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Transformation (Blue - White Screening) Teaching Kit
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Approx. Rs 4,950 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Laboratory |
| Form | Liquid |
| Material | Plastic |
| Shelf Life | 12 Months |
| I Deal In | New Only |
| Humidity Resistant | Yes |
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings and taken up through the cell membrane. Transformation occurs naturally in some species of bacteria, but it can also be affected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occuras a time-limited response to environmental conditions such as starvation and cell density. "Transformation" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because "transformation" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material.
| Cat. NO | Product | Experiments |
| OP-3164-5xp | Pro TechEx-Transformation (Blue - White Screening) Teaching Kit | 5 Exps |
| Components |
| Control Transformed |
| Bacteria culture (-20°C) |
| Host Bacteria (-20°C) |
| LB Agar (RT) |
| LB broth (RT) |
| Plasmid DNA (-20°C) |
| Solution- A (4°C) |
| 1.5ml Microfuge tubes (RT) |
| Ampicillin (4°C) |
| Glycerol(Sterile) (4°C) |
| IPTG (-20°C) |
| X-gal (-20°C) |
| Protocol |
Additional Information:
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PCR Teaching Kit
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Approx. Rs 5,900 / PieceGet Latest Price
Product BrochureProduct Details:| Minimum Order Quantity | 10 Piece |
| Number of Reactions(Preps)/Kit | 10 |
| Brand | Progene |
| Form | Kit |
| Shelf Life | 12 months |
| I Deal In | New Only |
| Humidity Resistant | Yes |
| Country of Origin | Made in India |
PCR is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3''-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the specific sequence will be accumulated in billions of copies (amplicons).
| CAT.NO | PRODUCT | EXPS |
| OP-3144-10xp | Pro TechEx-PCR Teaching Kit | 10 Exps |
| OP-3144-20xp | Pro TechEx-PCR Teaching Kit | 20 Exps |
Components
- Agarose (RT)
- 50X TAE (RT)
- Ethidium Bromide (RT)
- Gel Loading Dye (RT)
- Template DNA (-20°C)
- Primer(F R) (-20°C)
- Taq Polymerase (-20°C)
- 10 mM dNTP mix (-20°C)
- 10X Taq polymerase Buffer (-20°C)
- Distilled water (RT)
- Marker DNA (-20°C)
- PCR Tubes (RT)
- Protocol
Additional Information:
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Long PCR Teaching Kit
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Approx. Rs 7,500 / PieceGet Latest Price
Product BrochureProduct Details:| Usage/Application | Hospital |
| Size | N/A |
| Form | Kit |
| Material | N/A |
| I Deal In | New Only |
| Humidity Resistant | Yes |
Long PCR Amplification of extremely long PCR products (up to 40 kb DNA)Long Range PCR refers to the amplification of DNA lengths that cannot typically be amplified using routine PCR methods or reagents. For simple DNA templates, polymerases optimized for Long Range PCR can amplify up to 30 kb and beyond.
| CAT.NO | PRODUCT | EXPS |
| OP-3134-5xp | Pro TechEx-Long PCR Teaching Kit | 5 Exps |
| OP-3134-20xp | Pro TechEx-Long PCR Teaching Kit | 20 Exps |
- 50X TAE (RT)
- Agarose (RT)
- Ethidium Bromide (RT)
- Gel Loading Dye (RT
- Primer(F R) (-20 DegreeC)
- Taq Polymerase (-20 DegreeC)
- Template DNA (-20 DegreeC)
- 10 mM dNTP mix (-20 DegreeC)
- 10X Taq polymerase Buffer (-20 DegreeC)
- Distilled water (RT)
- Marker DNA (-20 DegreeC)
- PCR Tubes (RT)
- Protocol
20 Exp
Additional Information:
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Nested PCR Teaching Kit
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Approx. Rs 7,300 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Form | Liquid |
| Method | Elisa |
| Material | Plastic |
| I Deal In | New Only |
Nested PCR used to increase the specificity of DNA amplification. Two sets of primers are used in two successive reactions. In the first PCR, one pair of primers is used to generate DNA products, which may contain products amplified from non-target areas. The products from the first PCR are then used as template in a second PCR, using one ('hemi-nesting') or two different primers whose binding sites are located (nested) within the first set, thus increasing specificity. Nested PCR is often more successful in specifically amplifying long DNA products than conventional PCR, but it requires more detailed knowledge of the Sequence of the target.
| CAT.NO | PRODUCT | EXPS |
| OP-3138-5xp | Pro TechEx-Nested PCR Teaching Kit | 5 Exps |
| OP-3138-25xp | Pro TechEx-Nested PCR Teaching Kit | 25 Exps |
Components
- Agarose (RT)
- 50X TAE (RT)
- Ethidium Bromide (RT)
- Gel Loading Dye (RT)
- Template DNA (-20 DegreeC)
- Primer(F R) (-20 DegreeC)
- Taq Polymerase (-20 DegreeC)
- 10mM dNTP mix (-20 DegreeC)
- 10X Taq polymerase Buffer (-20 DegreeC)
- Distilled water (RT)
- Marker DNA (-20 DegreeC)
- PCR Tubes (RT)
- Protocol
25 Exp
Additional Information:
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Progene RT-PCR Teaching Kit
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Approx. Rs 8,500 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Usage/Application | Laboratory |
| Shelf Life | 12 Months |
| Result Time | 1 Day |
| Accuracy | 0.99 |
| Type | RT-PCR Teaching Kit |
| Country of Origin | Made in India |
Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike. However, they are separate and distinct techniques. While RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA, qPCR is used to quantitatively measure the amplification of DNA .
| Cat. NO | Product | Experiments |
| OP-3155-5xp | Pro TechEx-RT-PCR Teaching Kit | 5 Exps |
| Components | ||
| RNA Template (-20°C) | DNA Marker (-20°C) | 5x Reverse Transcriptase Buffer (-20°C) |
| 0.2ml PCR tubes (RT) | Primer F (-20°C) | M-MuLV Reverse transcriptase (-20°C) |
| 10mM DTT (-20°C) | Primer R (-20°C) | Ribonuclease Inhibitor (-20°C) |
| 50X TAE (RT) | Random Primer (-20°C) | Taq Polymerase Buffer (-20°C) |
| Agarose (RT) | Rnase Free Water (-20°C) | Protocol |
| Ethidium Bromide (RT) | Taq Polymerase (-20°C) | |
| Gel Loading dye (RT) | 10mM dNTP (-20°C) |
Additional Information:
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Multiplex PCR Teaching Kits
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Approx. Rs 6,000 / PieceGet Latest Price
Product BrochureProduct Details:| Method | PCR |
| Usage/Application | Laboratory |
| Disease | DNA |
| Brand | PROGENE |
| I Deal In | New Only |
| Country of Origin | Made in India |
Multiplex PCR uses several pairs of primers annealing to different target sequences. This permits the simultaneous analysis of multiple targets in a single sample. For example, in testing for genetic mutations, six or more amplifications might be combined. In the standard protocol for DNA Fingerprinting, the targets assayed are often amplified in groups of 3 or 4. Multiplex Ligation-dependent Probe Amplification (or MLPA) permits multiple targets to be amplified using only a single pair of primers, avoiding the resolution limitations of multiplex PCR. Multiplex PCR has also been used for analysis of microsatellites and SNPs.
| CAT.NO | PRODUCT | EXPS |
| OP-3136-5xp | Pro TechEx-Multiplex PCR Teaching Kit | 5 Exps |
| OP-3136-25xp | Pro TechEx-Multiplex PCR Teaching Kit | 25 Exps |
Components
- Agarose (RT)
- 50X TAE (RT)
- Ethidium Bromide (RT)
- Gel Loading Dye (RT)
- Template DNA (-20 DegreeC)
- Multiplex Primer(F R) (-20 DegreeC)
- Taq Polymerase (-20 DegreeC)
- 10 mM dNTP mix (-20 DegreeC)
- 10X Taq polymerase Buffer (-20 DegreeC)
- Distilled water (RT)
- Marker DNA (-20 DegreeC)
- PCR Tubes (RT)
- Protocol
Additional Information:
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PCR Application Teaching Kit
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Approx. Rs 6,900 / PieceGet Latest Price
Product BrochureProduct Details:| Brand | Progene |
| Result Time (Rapid Kits) | 1 Day |
| Method | PCR |
| Shelf Life | 12 Months |
| Test Time | 1 Day |
| Type | PCR Application Teaching Kit |
| Country of Origin | Made in India |
The polymerase chain reaction (PCR) has found widespread application in many areas of genetic analysis. For most, if not all of these applications, alternative methods of DNA (or RNA) amplification, can be substituted.The first application of PCR was for genetic testing, where a sample of DNA is analyzed for the presence of genetic disease mutations. Prospective parents can be tested for being genetic carriers, or their children might be tested for actually being affected by a disease. DNA samples for testing can be obtained by amniocentesis, chorionic villus sampling, or even by the analysis of rare fetal cells circulating in the mother's bloodstream. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations.
| CAT.NO | PRODUCT | EXPS |
| OP-3143-5xp | Pro TechEx-PCR Application Teaching Kit | 5 Exps |
| OP-3143-20xp | Pro TechEx-PCR Application Teaching Kit | 20 Exps |
Components
- Control DNA I (-20°C)
- Control DNA II (-20°C)
- Master mix vials (-20°C)
- Random primer (-20°C)
- Taq polymerase (-20°C)
- Test DNA (-20°C)
- 1Kb DNA ladder (-20°C)
- 50X TAE (RT)
- Agarose (RT)
- Ethidium bromide (RT)
- Gel loading Dye (RT)
- Protocol
Additional Information:
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