Laboratory Chemicals

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RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based buffer used for lysing red blood cells in single cell suspension with little to no effect on the nucleated cells.

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Pro CheMol–Lowry’s Reagent (125 ml) is a ready-to-use solution used in biotechnology and biochemistry for the quantitative estimation of proteins based on the Lowry method. This classical colorimetric assay combines the Biuret reaction (copper ion complex formation with peptide bonds) and the Folin–Ciocalteu reagent reaction, which produces a measurable blue color in proportion to protein concentration. The intensity of this color, typically measured at 750 nm, is directly related to the amount of protein present in the sample. Pro CheMol–Lowry’s Reagent is ideal for use in protein purification, enzyme assays, and biochemical analyses, providing high sensitivity and reproducibility compared to simpler assays like the Biuret or Bradford methods. It is compatible with a wide range of soluble proteins and peptides, though care is needed to avoid interference from detergents or strong reducing agents. Convenient and reliable, the 125 ml formulation is suitable for routine laboratory use in protein quantification and characterization studies.

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STET buffer is a lysis buffer commonly used in molecular biology for breaking open bacterial cells, particularly E. coli, during nucleic acid isolation and protein purification.

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Phosphate-citrate buffer is a common buffer used along with soluble horseradish peroxidase substrates such as o phenylenediaminedihydrochloride (OPD) or tetramethylbenzidine (TMB). It gives a pH of 5.0 with an excellent buffering capacity.

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RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.

Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

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