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Molecular Biology Teaching Kit

Offering you a complete choice of products which include pcr amplification teaching kit, pcr application teaching kit, plasmid purification by dna binding membrane teaching kit, southern blotting teaching kit, southern hybridization teaching kit and e.coli teaching kit.

PCR Amplification Teaching Kit

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PCR Amplification Teaching Kit
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The size of pUC19 will be increased to 5755bp with the addition of lacZ gene, which encodes ¿¿-galactosidase. The lacZ gene was amplified by PCR method. The amplified sequence was transformed to E.coli host .The production of ¿¿-galactosidase enzyme was analysed by SDS - PAGE method.
CAT.NOPRODUCTEXPS
OP-3142-5xpPro TechEx-PCR Amplification of ¿¿-Galactosidase Gene Teaching Kit5 Exps


Components

  • Agarose (RT)
  • 50X TAE (RT)
  • Ethidium Bromide (RT)
  • Gel Loading Dye (RT)
  • Template DNA (-20 DegreeC)
  • Primer (-20 DegreeC)
  • Taq Polymerase (-20 DegreeC)
  • 10 mM dNTP mix (-20 DegreeC)
  • 10X Taq polymerase Buffer (-20 DegreeC)
  • Distilled water (RT)
  • Marker DNA (-20 DegreeC)
  • PCR Tubes (RT)
  • Protocol

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20 Exp

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Additional Information:

  • Item Code: beta-Galactosidase
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    PCR Application Teaching Kit

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    PCR Application Teaching Kit
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    MOQ: 20 Exps

    The polymerase chain reaction (PCR) has found widespread application in many areas of genetic analysis. For most, if not all of these applications, alternative methods of DNA (or RNA) amplification, can be substituted.The first application of PCR was for genetic testing, where a sample of DNA is analyzed for the presence of genetic disease mutations. Prospective parents can be tested for being genetic carriers, or their children might be tested for actually being affected by a disease. DNA samples for testing can be obtained by amniocentesis, chorionic villus sampling, or even by the analysis of rare fetal cells circulating in the mother's bloodstream. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations.

    CAT.NOPRODUCTEXPS
    OP-3143-5xpPro TechEx-PCR Application Teaching Kit5 Exps
    OP-3143-20xpPro TechEx-PCR Application Teaching Kit20 Exps

    Components
    • Control DNA I (-20°C)
    • Control DNA II (-20°C)
    • Master mix vials (-20°C)
    • Random primer (-20°C)
    • Taq polymerase (-20°C)
    • Test DNA (-20°C)
    • 1Kb DNA ladder (-20°C)
    • 50X TAE (RT)
    • Agarose (RT)
    • Ethidium bromide (RT)
    • Gel loading Dye (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3143-20xp
  • Yes! I am interested

    Plasmid Purification By DNA Binding Membrane Teaching Kit

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    Plasmid Purification By DNA Binding Membrane Teaching Kit
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    MOQ 20 Exps

    After harvesting and resuspension, the bacterial cells are lysed in NaOH-SDS in the presence of RNase A. SDS solubilizes the phospholipid and protein components of the cell membrane, leading to lysis and release of the cell contents. NaOH denatures the chromosomal and plasmid DNAs, as well as proteins. The optimized lysis time allows maximum release of plasmid DNA from the cell without release of cell wall-bound chromosomal DNA, while minimizing the exposure of the plasmid to denaturing conditions. Long exposure to alkaline conditions may cause the plasmid to become irreversibly denatured. This denatured form of the plasmid runs faster on agarose gels and is resistant to restriction enzyme digestion. The lysate is neutralized by the addition of acidic potassium acetate. The high salt concentration causes KDS* to precipitate, and the denatured proteins, chromosomal DNA, and cellular debris become trapped in salt-detergent complexes.
    CAT.NOPRODUCTEXPS
    OP-3148-10xpPro TechEx- Plasmid Purification By DNA Binding Membrane Teaching Kit10 Exps
    Components
    • 2ml Collection Vial (RT)
    • Column (RT)
    • Elution Buffer (RT)
    • Gel loading dye (RT)
    • Solution I (RT)
    • Solution II (RT)
    • Solution III (RT)
    • Wash Solution (RT)
    • 50X TAE (RT)
    • Agarose (RT)
    • Ampicillin_ (4 DegreeC)
    • DNA Marker (-20 DegreeC)
    • E.coli Host (4 DegreeC)
    • Ethidium Bromide (RT)
    • RNase A (-20 DegreeC)
    • Protocol
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    Southern Blotting Teaching Kit

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    Southern Blotting Teaching Kit
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    Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1975. It allows investigators to determine the molecular weight of a restriction fragment, to measure relative amounts in different samples and to locate a particular sequence of DNA within a complex mixture. DNA (genomic or other source) is digested with a restriction enzyme and separated by gel electrophoresis and transferred from an agarose gel onto a membrane.

    CAT.NOPRODUCTEXPS
    OP-3158-5xpPro TechEx-Southern Blotting Teaching Kit5 Exps
    OP-3158-20xpPro TechEx-Southern Blotting Teaching Kit20 Exps


    Components
    • 50X TAE (RT)
    • Agarose (RT)
    • DNA sample (-20 DegreeC)
    • Ethidium Bromide (RT)
    • Gel loading dye (RT)
    • 20X SSC (RT)
    • Nitrocellulose Membrane (RT)
    • Protocol

    MOQ-20 Exps


    Additional Information:

  • Item Code: OP-3158-20xp
  • Yes! I am interested

    Southern Hybridization Teaching Kit

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    Southern Hybridization Teaching Kit
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    MOQ 5 Exps

    Hybridization involves several steps. First, the probe and sample DNA are allowed to hybridize under the appropriate conditions. The correct temperature, incubation times, and buffer conditions must be used. Next, the sample DNA is washed using conditions that will remove unhybridized probe but not the hybridized probe. Finally, the sample DNA is tested for the presence of the hybridized probe. The probe is labeled with a radioactive molecular tag (or some other tag) that allows it to be detected following hybridization.
    CAT.NOPRODUCTEXPS
    OP-3159-5xpPro TechEx-Southern Hybridization Teaching Kit5 Exps

    Components
    • 10X Safe Blue DNA Stain (-20 DegreeC)
    • 50X TAE (RT)
    • Agarose (RT)
    • Control DNA (RT)
    • DNA Sample I (RT)
    • DNA Sample II (RT)
    • EthidiumBromide(RT)
    • 10X Substrate (RT)
    • 5X Wash Buffer-A (RT)
    • 5X Wash Buffer-B (RT)
    • Biotinylated Probe (RT)
    • HRP-Conjugate (RT)
    • Hybridization Buffer (RT)
    • Prehybridization Buffer (RT)
    • 50X Electrotransfer Buffer (RT)
    • 5X Blocking Buffer (RT)
    • 5X Wash Buffer-C (RT)
    • Blocking Powder (RT)
    • Casein (RT)
    • Control Nylon Membrane (RT)
    • Nylon Membrane (RT)
    • Protocol
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    E.Coli Teaching Kit

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    E.Coli Teaching Kit
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    Physical and Chemical Mutagens on E.coli teaching kit Pro Tech Ex-Study of Physical and Chemical Mutagens on The Su UV Light.

    UV light which has a lower energy content than ionizing radiations, are capable of producing lethal effects in cells exposed to low penetrating wavelengths in range of 210 to 300nm.
    Cellular components capable of absorbiong UV light are nucleic acids with the DNA as the primary site of damage. this results in the inabilityy to form viable cells after exposure to UV radiation.
    Hydroxylamine is used to mutagenize DNA in Vitro, when used in vitro, Hydroxylamine reacts with cytosine, converting it to a modified base that pairs with adenine.
    This has two consequences that is Hydroxylamine only produces GC to AT transitions, and mutations induced by hydroxylamine cannot be reverted with hydroxylamine.

    CAT.NOPRODUCTEXPS
    OP-3162-5xpPro TechEx-Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit5 Exps

    Components
    • Agar (RT)
    • E.coli host (4 DegreeC)
    • Hydroxylamine (RT)
    • LB broth (RT)
    • Saline (RT)
    • sodium hydroxide (RT)
    • Protocol
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    Restriction Digestion Teaching Kit

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    Restriction Digestion Teaching Kit
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    Restriction enzymes are Nucleases which can cleave the sugar-phosphate backbone of DNA, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They specifically cleave the nucleic acids at specific nucleotide sequence called Restriction sites to generate a set of smaller fragments.

    CAT.NOPRODUCTEXPS
    OP-3153-5xpPro TechEx-Restriction Digestion Teaching Kit5 Exps
    OP-3153-20xpPro TechEx-Restriction Digestion Teaching Kit20 Exps

    Components

    • 50X TAE (RT)
    • Agarose (RT)
    • Ethidium bromide (RT)
    • Gel loading dye (RT)
    • Template DNA (-20 DegreeC)
    • 0.5ml tubes (RT)
    • Enzyme Buffer (-20 DegreeC)
    • Hind III (-20 DegreeC)
    • Sterile water (RT)
    • Protocol

    20 Exp


    Additional Information:

  • Item Code: OP-3153-20xp
  • Yes! I am interested

    Single Nucleotide Polymorphisms Teaching Kit

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    Single Nucleotide Polymorphisms Teaching Kit
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    MOQ 20 Exps

    Genetic disorders and other related diseases are often associated with Single Nucleotide Polymorphism (SNP) occurring at precise positions on genes/ genomes. Development of SNP-specific primers that amplify a specific fragment in PCR when a specific SNP is present helps in disease detection.SNP detection where two DNA templates, one wild-type and the other template with SNP are used for PCR amplification individually along with 2 pairs (4 numbers) of primers, one pair specific to wild-type and the other, specific to SNP-type primer.
    CAT.NOPRODUCTEXPS
    OP-3156-5xpPro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit5 Exps
    OP-3156-20xpPro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit20 Exps
    Components

    • Control DNA (-20 DegreeC)
    • DNA Marker (-20 DegreeC)
    • dNTP Mix (-20 DegreeC)
    • Primer Mix* (-20 DegreeC)
    • SNP DNA (-20 DegreeC)
    • Taq DNA Polymerase(-20 DegreeC)
    • Taq DNA Polymerase
    • Buffer 10X (-20 DegreeC)
    • 0.2ml PCR Tube (RT)
    • 50X TAE (RT)
    • Agarose (RT)
    • Distilled water (RT)
    • Ethidium Bromide (RT)
    • Gel loading Dye (RT)
    • Protocol

    Additional Information:

  • Item Code: SNP
  • Yes! I am interested

    DNA Methylation Teaching Kit

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    DNA Methylation Teaching Kit
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    DNA methylation is a biochemical process where a methyl group is added to the cytosine or adenine DNA nucleotides.
    CAT.NOPRODUCTEXPS
    OP-3160-5xpPro TechEx-Study of DNA Methylation Teaching Kit5 Exps

    Components
    • EcoRI Methylase Enzyme (-20 DegreeC)
    • 10X Restriction Buffer (-20 DegreeC)
    • Lambda DNA (-20 DegreeC)
    • Methylase Buffer (10X) (-20 DegreeC)
    • Restriction enzyme (EcoRI) (-20 DegreeC)
    • S-Adenosyl methionine (SAM) (-20 DegreeC)
    • 50X TAE (RT)
    • Agarose (RT)
    • Distilled Water(-20 DegreeC)
    • Ethidium Bromide (RT)
    • Gel loading Dye (RT)
    • Microfuge Tubes (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3160-5xp
  • Yes! I am interested

    Transduction Teaching Kit

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    Transduction Teaching Kit
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    MOQ 20 Exps

    Transduction is the process by which DNA is transferred from one bacterium to another by a virus. It also refers to the process whereby foreign DNA is introduced into another cell via a viral vector. Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation), and it is DNase resistant (transformation is susceptible to DNase). Transduction is a common tool used by molecular biologists to stably introduce a foreign gene into a host cell's genome.

    CAT.NOPRODUCTEXPS
    OP-3163-5xpPro TechEx-Transduction Teaching Kit5 Exps
    OP-3163-20xpPro TechEx-Transduction Teaching Kit20 Exps
    Components
    • (4 DegreeC)
    • Donar strain (-20 DegreeC)
    • Host strain (-20 DegreeC)
    • (4 DegreeC)
    • Phage lysate (-20 DegreeC)
    • Recipient strain (-20 DegreeC)
    • 10% Dextrose (4 DegreeC)
    • 1M Calcium chloride (RT)
    • 1M Magnesium chloride (RT)
    • Agar (RT)
    • LB Broth (RT)
    • Protocol

    Additional Information:

  • Item Code: OP-3163-20xp
  • Yes! I am interested

    PCR Molecular Biology Teaching Kit

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    PCR Molecular Biology Teaching Kit
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    PCR is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the specific sequence will be accumulated in billions of copies (amplicons).

    CAT.NOPRODUCTEXPS
    OP-3144-10xpPro TechEx-PCR Teaching Kit10 Exps
    OP-3144-20xpPro TechEx-PCR Teaching Kit20 Exps

    Components

    • Agarose (RT)
    • 50X TAE (RT)
    • Ethidium Bromide (RT)
    • Gel Loading Dye (RT)
    • Template DNA (-20°C)
    • Primer(F R) (-20°C)
    • Taq Polymerase (-20°C)
    • 10 mM dNTP mix (-20°C)
    • 10X Taq polymerase Buffer (-20°C)
    • Distilled water (RT)
    • Marker DNA (-20°C)
    • PCR Tubes (RT)
    • Protocol
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    Restriction Analysis of Plasmid

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    Restriction Analysis of Plasmid
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    Restriction enzymes (Hind III) are Nucleases which can cleave the sugar-phosphate backbone of pBR 322 & pUC 18, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They specifically cleave the nucleic acids at specific nucleotide sequence called Restriction sites to generate a set of smaller fragments.
    CAT.NOPRODUCTEXPS
    OP-3152-5xpPro TechEx-Restriction Analysis of Plasmid (pBR322 & pUC 18) Teaching Kit5 Exps

    Components
    • Agarose (RT)
    • 50X TAE (RT)
    • Gel loading Dye (RT)
    • Ethidium Bromide (RT)
    • 0.5ml Microfuge tubes (RT)
    • pBR322 (-20 DegreeC)
    • pUC18 (-20 DegreeC)
    • HindIII (-20 DegreeC)
    • Restriction Buffer (-20 DegreeC)
    • Distilled Water (-20 DegreeC)
    • Protocol

    5 Exp


    Additional Information:

  • Item Code: pBR322-pUC18
  • Yes! I am interested

     
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